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Adipose Triglyceride Lipase (ATGL) ELISA

Alternative name:

  • Patatin-like phospholipase domain-containing protein 2
  • Calcium-independent phospholipase A2
  • Desnutrin
  • Pigment epithelium-derived factor
Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes
BACKGROUND: In adipose cells, adipose triglyceride lipase (ATGL) catalyzes the first step in adipocyte triacylglyceride hydrolysis, thereby regulating both basal and hormone-stimulated lipolysis. However, little is known about the molecular mechanism(s) underlying habitual exercise-induced adaptive modulation of ATGL in white adipocytes via alteration in transcription regulator and lipolytic cofactors.
METHODOLOGY/PRINCIPAL RESULTS: Male Wistar rats were randomly divided into 2 groups a sedentary control group (CG) and a habitual exercise group (EG). The EG was subjected to running on a treadmill set at 5 days per week for 9 weeks. The CG was not subjected to running on a treadmill. In the EG, levels of ATGL mRNA and protein were elevated with a significant increase in lipolysis compared with the CG, accompanied by a significant increase in associations of CGI-58 with ATGL protein. Under these conditions, an upregulation of peroxisome proliferation-activated receptorg-2 (PPARg-2) was observed. In the EG, the addition of rosiglitazone further significantly increased the levels of ATGL protein compared with the CG. However, attenuated levels of the ATGL protein in adipocytes were obtained by the addition of insulin, which is known to inhibit the expression of ATGL in both types of groups. Actually, levels of plasma insulin were significantly reduced in the EG compared with the CG.
CONCLUSIONS:These data suggest that elevated levels of ATGL are involved in the exercise-induced enhancement of lipolysis in primary adipocytes. The exact mechanism(s) underlying this phenomenon is associated, at least in part, with upregulated transcriptional activation of PPARg-2. In addition, exercise-induced lower circulation levels of insulin also correlate with habitual exercise-induced higher levels of ATGL in primary adipocytes.
Ogasawara J et al. PLoS One.2012;7(7)
 
 
 
human ATGL elisa kit for cell cultures samples assay from aviscera bioscience Human ATGL  ELISA Kit
Code No.: SK00576-01
Size: 96 T
Price: $420.00 USD
Standard Range: 0.78-100 ng/ml
Sensitivity: 200 pg/ml
Sample Type: tissue homogenates, cell cultures
Sample require: 100ul per well
Protein ID :Q96AD5
Gene ID : 57104
Intra-CV: 4-6%
Inter-CV: 8-12%
Data Sheet: PDF
Human ATGL  His Tag Rec
Code No.: 00576-01-100
Size: 96 T
Price: $360.00 USD
Protein ID :Q96AD5
Gene ID : 57104
Tag; His Tag on N-terminus
MW: 73 KD
Data Sheet: PDF
Anti Human ATGL Antibody
Code No.: A00576-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Antigen: human ATGL, Rec
Ab Type: Purified IgG
Purification: Protein A
Applications: E, IHC, WB
Working Dilution: IHC 2-4 ug/ml
Data Sheet: PDF
Anti Human ATGL Antibody
Code No.: A00576-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Antigen: human ATGL, Rec
Ab Type: Purified IgG
Purification: Protein A
Applications: E, IHC, WB
Working Dilution: IHC 2-4 ug/ml
Data Sheet: PDF
Anti Human ATGL Monoclonal Antibody
Code No.: A00576-06-100
Size: 100 ug
Price: $260.00 USD
Host: Mouse
Antigen: human ATGL rec
Ab Type: Purified IgG
Purification: Protein G
Applications: E
Working Dilution: 0.2 ug/ml)
Data Sheet: PDF
 

 

Name
Code No.
Size
Price ($)
ATGL (Human)ELISA Kit
96 T
420.00
ATGL (Human)Recombinant
10 ug
80.00
ATGL (Human)Recombinant
100 ug
360.00
ATGL (Human) Recombinant
100 ug
360.00
Anti Human ATGL IgG
100 ug
220.00
Anti ATGL (Human)  Monoclonal Antibody
A00576-06-100
100 ug
260.00