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Bone Morphogenetic Protein 4 (BMP4)ELISA Kit
 
 
BMP4 is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. These proteins are synthesized as preproBMPs, cleaved, and then processed into dimeric proteins. BMP4 induces bone and cartilage formation.
 

BMP4-mediated brown fat-like changes in white adipose tissue alter glucose and energy homeostasis

Expression of bone morphogenetic protein 4 (BMP4) in adipocytes of white adipose tissue (WAT) produces “white adipocytes” with characteristics of brown fat and leads to a reduction of adiposity and its metabolic complications. Although BMP4 is known to induce commitment of pluripotent stem cells to the adipocyte lineage by producing cells that possess the characteristics of preadipocytes, its effects on the mature white adipocyte phenotype and function were unknown. Forced expression of a BMP4 transgene in white adipocytes of mice gives rise to reduced WAT mass and white adipocyte size along with an increased number of a white adipocyte cell types with brown adipocyte characteristics comparable to those of beige or brite adipocytes. These changes correlate closely with increased energy expenditure, improved insulin sensitivity, and protection against diet-induced obesity and diabetes. Conversely, BMP4-deficient mice exhibit enlarged white adipocyte morphology and impaired insulin sensitivity. We identify peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC1α) as the target of BMP signaling required for these brown fat-like changes in WAT. This effect of BMP4 on WAT appears to extend to human adipose tissue, because the level of expression of BMP4 in WAT correlates inversely with body mass index. These findings provide a genetic and metabolic basis for BMP4’s role in altering insulin sensitivity by affecting WAT development
Shu-Wen Qian, et al. PNAS 2013 ; published ahead of print February 6, 2013, doi:10.1073/pnas.1215236110

Identification of BMP and Activin Membrane-Bound Inhibitor (BAMBI) as a Potent Negative Regulator of Adipogenesis and Modulator of Autocrine/Paracrine Adipogenic Factors

Adipose tissue dysfunction underpins the association of obesity with type 2 diabetes. Adipogenesis is required for the maintenance of adipose tissue function. It involves the commitment and subsequent differentiation of preadipocytes and is coordinated by autocrine, paracrine, and endocrine factors. We previously reported that fibroblast growth factor-1 (FGF-1) primes primary human preadipocytes and Simpson Golabi Behmel syndrome (SGBS) preadipocytes and increases adipogenesis through a cascade involving extracellular signal–related kinase 1/2 (ERK1/2). Here, we aimed to use the FGF-1 system to identify novel adipogenic regulators. Expression profiling revealed bone morphogenetic protein (BMP) and activin membrane-bound inhibitor (BAMBI) as a putative FGF-1 effector. BAMBI is a transmembrane protein and modulator of paracrine factors that regulate adipogenesis, including transforming growth factor (TGF) superfamily members (TGF-β and BMP) and Wnt. Functional investigations established BAMBI as a negative regulator of adipogenesis and modulator of the anti- and proadipogenic effects of Wnt3a, TGF-β1, and BMP-4. Further studies showed that BAMBI expression levels are decreased in a mouse model of diet-induced obesity. Collectively, these findings establish BAMBI as a novel, negative regulator of adipogenesis that can act as a nexus to integrate multiple paracrine signals to coordinate adipogenesis. Alterations in BAMBI may play a role in the (patho)physiology of obesity, and manipulation of BAMBI may present a novel therapeutic approach to improve adipose tissue function.
Xiao Luo, et al. Diabetes January 2012 61:124-136; doi:10.2337/db11-0998
 
 
Human BMP4 ELISA Kit from aviscerbioscience
Human BMP4 ELISA Kit
Code No.: SK00014-01
Size: 96 T
Price: $320.00 USD
Standard range: 7.8-500 pg/ml
Sensitivity: 3 pg/ml
Sample type: serum, plasma, cell cultures
Sample require: 100uL per well
Intra-CV: 4-6%
Inter-CV:8-10%
Protocol: PDF

Human BMP4 Recombinant
Code No.: 00014-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID:P12644
Gene ID: 652
MW: 14.9 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 90%
Data Sheet: PDF

 

Anti Human BMP4 IgG
Code No.: A00014-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Antigen: human BMP4, Rec.
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: E, IHC (human kidney tissue)
Working Dilution: 1000
Data Sheet: PDF

 

Name
Code No.
Size
Price ($)
BMP-4 (Human) ELISA Kit SK00014-01 96 T 320.00
BMP4 (Human), Rec.
00014-01-50
50 ug
180.00
BMP4 (Human), Rec.
100 ug
260.00
BMP4 (Human), Rec.
00014-01-1000
1 mg
2200.00
BMP4 (Human), Rec. Biotinylated
00014-01-50B
50 ug
395.00
BMP4 (Human), Rec. . FITC Conjugated
50 ug
395.00
BMP4 (Human), Rec. Cy3 Conjugated
00014-01-50C3
50 ug
495.00
Rabbit Anti BMP4 (H) IgG
100 ug
220.00