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Human Soluble CD14 ELISA KIt
Human Presepsin ELISA Kit

Role of presepsin for the evaluation of sepsis in the emergency department

Abstract Sepsis, severe sepsis and septic shock are among the most common conditions handled in the emergency department (ED). According to new Sepsis Guidelines, early diagnosis and treatment are the keys to improve survival. Plasma C-reactive protein (CRP) and procalcitonin (PCT) levels, when associated with documented or suspected infection, are now part of the definitions of sepsis. Blood culture is the gold standard method for detecting microorganisms but it requires too much time for results to be known. Sensitive biomarkers are required for early diagnosis and as indexes of prognosis sepsis. CRP is one of the acute phase proteins synthesized by the liver: it has a great sensitivity but a very poor specificity for bacterial infections. Moreover, the evolution of sepsis does not correlate with CRP plasma changes. In recent years PCT has been widely used for sepsis differential diagnosis, because of its close correlation with infections, but it still retains some limitations and false positivity (such as in multiple trauma and burns). Soluble CD14 subtype (sCD14-ST), also known as presepsin, is a novel and promising biomarker that has been shown to increase significantly in patients with sepsis, in comparison to the healthy population. Studies pointed out the capability of this biomarker for diagnosing sepsis, assessing the severity of the disease and providing a prognostic evaluation of patient outcome. In this mini review we mainly focused on presepsin: we evaluate its diagnostic and prognostic roles in patients presenting to the ED with systemic inflammatory response syndrome (SIRS), suspected sepsis or septic shock.
Pizzolato E et al. Clin Chem Lab Med. 2014 Jun 4. pii: /j/cclm.ahead-of-print/cclm-2014-0199/cclm-2014-0199.xml. doi: 10.1515/cclm-2014-0199. [Epub ahead of print]

Usefulness of presepsin (sCD14-ST) measurements as a marker for the diagnosis and severity of sepsis that satisfied diagnostic criteria of systemic inflammatory response syndrome

CD14 is present in macrophage, monocyte, and granulocyte cells and their cell membranes, and it is said to be responsible for intracellular transduction of endotoxin signals. Its soluble fraction is present in blood and is thought to be produced in association with infections. It is called the soluble CD14-subtype (sCD14-ST), and in the following text it is referred to by its generic name, presepsin. We have previously reported that presepsin is produced in association with infection and that it is specifically expressed in sepsis. In the present study we developed a new rapid diagnostic method by using a chemiluminescent enzyme immunoassay that allowed making automated measurements in a shorter time. The results of using this method to measure presepsin values in different pathological conditions were normal, 294.2 ± 121.4 pg/ml; local infection, 721.0 ± 611.3 pg/ml; systemic inflammatory response syndrome, 333.5 ± 130.6 pg/ml; sepsis, 817.9 ± 572.7 pg/ml; and severe sepsis, 1,992.9 ± 1509.2 pg/ml; the presepsin values were significantly higher in patients with local infection, sepsis, and severe sepsis than in patients who did not have infection as a complication. In a comparative study with other diagnostic markers of sepsis based on ROC curves, the area under the curve (AUC) of presepsin was 0.845, and greater than the AUC of procalcitonin (PCT, 0.652), C-reactive protein (CRP, 0.815), or interleukin 6 (IL-6, 0.672). In addition, a significant correlation was found between the APACHE II scores, an index of disease severity, and the presepsin values, suggesting that presepsin values can serve as a parameter that closely reflects the pathology.
Shozushima T et al. J Infect Chemother. 2011 Dec;17(6):764-9

Diagnostic value of soluble CD14 subtype (sCD14-ST) presepsin for the postmortem diagnosis of sepsis-related fatalities

he first aim of this study was to assess the diagnostic performance of presepsin (sCD14-ST) in postmortem serum from femoral blood compared to procalcitonin (PCT) to detect sepsis-related fatalities. The second aim was to compare sCD14-ST levels found in postmortem serum to the values in pericardial fluid to investigate the usefulness of the latter as an alternative biological fluid. Two study groups were formed, a sepsis-related fatalities group and a control group. Radiology (unenhanced CT scans and postmortem angiographies), autopsies, histology, neuropathology, and toxicology as well as other postmortem biochemistry investigations were performed in all cases. Microbiological investigations on right cardiac blood were carried out exclusively in septic cases. The results of this study indicated that postmortem serum PCT and sCD14-ST levels, individually considered, allowed septic cases to be identified. Even though increases in both PCT and sCD14-ST concentrations were observed in the control cases, coherent PCT and sCD14-ST results in cases with suspected sepsis allowed the diagnosis to be confirmed. Conversely, no relevant correlation was identified between postmortem serum and pericardial fluid sCD14-ST levels in either the septic or control groups.
Palmiere C et al. Int J Legal Med. 2013 Jul;127(4):799-808.
cd4 elisa kit assay
soluble CD14 ELISA Kit
soluble cd14 elisa kit from aviscera Human Soluble CD14 Ultrasensitive ELISA Kit
Code No.: SK00178-06
Size: 96 T
Price: $460.00 USD
Standard Range:19-1250 pg/ml
Sensitivity: 7 pg/ml
Sample Type: serum, plasma
Sample Require: 10 uL of serum or plasma
Dilution factor: 2000 ~ 4000
Intra CV: 4-6%
Inter CV: 8-10%
Protocol: PDF
CD14 ELISA from aviscera Human Soluble CD14 ELISA Kit (192T)
Code No.: SK00178-01B
Size: 192 T
Price: $720.00 USD
Standard Range:39-2500 pg/ml
Sensitivity: 10 pg/ml
Sample Type: serum, plasma
Sample Require: 10 uL of serum or plasma
Dilution factor: 2000 ~ 4000
Intra CV: 4-6%
Inter CV: 8-10%
Protocol: PDF
mouse sCD14 elisa kit SK00178-03 Soluble CD14 (Mouse) ELISA Kit
Code No.: SK00178-03
Size: 96 T
Price: $420.00 USD
Standard Range:78-5000 pg/ml
Sensitivity: 20 pg/ml
Sample Type: serum, plasma
Sample Volume: 100 uL
Intra CV: 4-6%
Inter CV: 8-10%
Protocol: PDF
Soluble CD14 (Human) Recombinant
Code No.: 00178-02-10
Size: 10 ug
Price: $190.00 USD
Protein ID:NP_000582.1
Gene ID: NM_000591.3.
Sequence: 20Thr-344Met
Tag: His Tag on its-C-terminus
Expression: HEK293 cells
MW: 45-50 KD
Purity: more than 95% in SDS-PAGE
Protocol: PDF
Soluble CD14 (Human) Biotinylated
Code No.: 00178-02-20B
Size: 20 ug
Price: $490.00 USD
Protein ID:NP_000582.1
Gene ID: NM_000591.3.
Sequence: 20Thr-344Met
Tag: His Tag on its C-terminus
Expression: HEK293 cells
MW: 45-50 KD
Purity: more than 95% in SDS-PAGE
Protocol: PDF
Soluble CD14 (Human) Fc fusion
Code No.: 00178-04-10
Size: 10 ug
Price: $190.00 USD
Protein ID:NP_000582.1
Gene ID: NM_000591.3.
Sequence: 20Thr-352Cys
Tag: human IgG1 Fc on its C-terminus
Expression: HEK293 cells
MW: 80-85 KD
Purity: more than 95% in SDS-PAGE
Protocol: PDF

 

Name
Code No.
Size
Price ($)
Soluble CD14  (Human) Ultrasensitive ELISA Kit
96 T
460.00
Soluble CD14  (Human) ELISA Kit (192T)
192 T
720.00
Soluble CD14  (Human) ELISA Kit (480T)
480 T
1750.00
Soluble CD14  (Human) ELISA Kit (960T)
960 T
3300.00
Soluble CD14  (Mouse) ELISA Kit
96 T
420.00
Soluble CD14  (Human) His Tag (HEK293)
00178-02-10
10 ug
190.00
Soluble CD14  (Human) His Tag (HEK293) Biotinylated
00178-02-20B
20 ug
490.00
Soluble CD14  (Human) Fc Fusion (HEK293)
00178-04-10
10 ug
190.00

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