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Soluble CD19ELISA
 

 

 

The CD19 signalling molecule is elevated in NOD mice and controls type 1 diabetes development

AIMS/HYPOTHESIS: Type 1 diabetes is characterised by early peri-islet insulitis and insulin autoantibodies, followed by invasive insulitis and beta cell destruction. The immunological events that precipitate invasive insulitis are not well understood. We tested the hypothesis that B cells in diabetes-prone NOD mice drive invasive insulitis through elevated expression of CD19 and consequent enhanced uptake and presentation of beta cell membrane-bound antigens to islet invasive T cells.
METHODS: CD19 expression and signalling pathways in B cells from NOD and control mice were compared. Expansion of CD8(+) T cells specific for insulin and islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP) were compared in CD19-deficient and wild-type NOD mice and this was correlated with insulitis severity. The therapeutic potential of anti-CD19 treatment during the period of T cell activation was assessed for its ability to block invasive insulitis.
RESULTS: CD19 expression and signalling in B cells was increased in NOD mice. CD19 deficiency significantly diminished the expansion of CD8(+) T cells with specificity for the membrane-bound beta cell antigen, IGRP. Conversely the reduction in CD8(+) T cells with specificity for the soluble beta cell antigen, insulin, was relatively small and not significant.
CONCLUSIONS/INTERPRETATION: Elevated CD19 on NOD B cells promotes presentation of the membrane-bound antigen, IGRP, mediating the expansion of autoreactive T cells specific for antigens integral to beta cells, which are critical for invasive insulitis and diabetes. Downregulating theCD19 signalling pathway in insulin autoantibody-positive individuals before the development of type 1 diabetes may prevent expansion of islet-invasive T cells and preserve beta cell mass.
Ziegler AI et al. Diabetologia. 2013 Dec;56(12):2659-68.
 
   
  Human Soluble CD19 ELISA Kit
Code No.: SK00431-01
Size: 96 T
Price: $420.00 USD
Standard range 456-15000 pg/ml
Sensitivity: 456 pg/ml
Sample Type: serum, plasma
sample require:100 uL per well
Intra-CV: 4-6%
Inter-CV: 8-10%
Data Sheet: PDF

 

Human Soluble CD19 Recombinant
Code No.: 00431-01-10
Size: 10 ug
Price: $220.00 USD
Protein ID:NP_001171569
Gene ID: NM_001178098
MW:47 KD
Tag: His Tag on C-Terminus
Expressed: 293 cells derived
Purity: 90%
Data Sheet: PDF
Anti Human Soluble CD69 Monoclonal Antibody
Code No.: A00431-06-50
Size: 50 ug
Price: $290.00 USD
Host: mouse
Antigen: human CD19 ECD
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: FCM
Working Dilution: 1ug/test
Data Sheet: PDF

 

 

Name
Code No.
Size
Price ($)
Soluble CD19 (Human) ELISA Kit SK00431-01 96 T 420.00
Soluble CD19(Human) Recombinant
00431-02-10
10 ug
220.00
Mouse Anti Soluble CD19 (Human) Monoclonal Antibody
A00431-06-50
50 ug
280.00