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Soluble CD36 ELISA Kits
A biomarker for obesity and cardiovasculardiseases
BACKGROUND:Cardiovascular disease (CVD) results from a combination of abnormalities in lipoprotein metabolism, oxidative stress, chronic inflammation, and susceptibility to thrombosis. Atherosclerosis is the major cause of CVD. CD36 has been shown to play a critical role in the development of atherosclerotic lesions by its capacity to bind and promote endocytosis of oxidized low-density lipoprotein (oxLDL) and is implicated in the formation of foam cells. The purpose of this research was to evaluate whether there is an association of sCD36 and oxLDL levels with cardiovascular risk factors in young subjects.
METHODS:A total of 188 subjects, 18 to 25 years old, 133 normal-weight and 55 obese subjects from the state of Guerrero, Mexico were recruited in the study. The lipid profile and glucose levels were measured by enzymatic colorimetric assays. Enzyme-linked immunosorbant assays (ELISA) for oxLDL and sCD36 were performed. Statistical analyses of data were performed with Wilcoxon- Mann Whitney and chi-square tests as well as with multinomial regression.
RESULTS:TC, LDL-C, TG, oxLDL and sCD36 levels were higher in obese subjects than in normal-weight controls, as well as, monocyte and platelet counts (P < 0.05). Obese subjects had 5.8 times higher risk of sCD36 in the third tertil (>97.8 ng/mL) than normal-weight controls (P = 0.014), and 7.4 times higher risk of oxLDL levels in third tertile (>48 U/L) than control group. The subjects with hypercholesterolemia, hypertriglyceridemia, fasting impaired LDL-C had a higher risk of oxLDL levels in the third tertile (>48 U/L) than the control group (P < 0.05).
CONCLUSIONS:Circulating CD36 and oxLDL levels are associated with cardiovascular risk factors in young subjects and may be potential early markers for cardiovascular disease (CVD).
Ramos-Arellano LE et al. BMC Cardiovasc Disord. 2014 Apr 28;14:54. doi: 10.1186/1471-2261-14-54.

Human Soluble CD36 ELISA Kit was used by Dr.  Parra-Rojas Iand Ramos-Arellano LE, Laboratorio de Investigación en Obesidad y Diabetes, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, México. iprojas@yahoo.com.

Soluble CD36 (sCD36) clusters with markers of insulin resistance, and high sCD36 is associated with increased type 2 diabetes risk
CONTEXT AND OBJECTIVE: Soluble CD36 (sCD36) may be an early marker of insulin resistance and atherosclerosis. The objective of this prospective study was to evaluate sCD36 as a predictor of type 2 diabetes and to study its relationship with components of the metabolic syndrome (MetSy). DESIGN, SETTING, PARTICIPANTS, AND OUTCOME MEASURES: We conducted a case-referent study nested within a population-based health survey. Baseline variables included sCD36, body mass index, blood pressure, blood lipids, adipokines, inflammatory markers, and beta-cell function. A total of 173 initially nondiabetic cohort members who developed type 2 diabetes during 10 yr of follow-up were matched (1:2) with referents. Exploratory factor analysis was applied to hypothesize affiliation of sCD36 to the MetSy components. RESULTS: Doubling of baseline sCD36 increases the odds ratio for diabetes development by 1.24 in the general study population and by 1.45 in the female population (P < 0.025). Comparing upper sCD36 quartiles with lower, odds ratio for diabetes was 4.6 in women (P = 0.001), 3.15 in men (P = 0.011), and 2.6 in obese individuals (P < 0.025). Multivariate analysis shows that sCD36 does not predict diabetes independent of fasting plasma glucose and insulin. Factor analysis of 15 variables generates a six-factor model explaining 66-69% of total variance, where sCD36, body mass index, insulin, proinsulin, and leptin were assigned to the obesity/insulin resistance cluster. CONCLUSIONS: Upper quartile sCD36 is associated with elevated diabetes risk independent of age, gender, and obesity. Baseline sCD36 does not, however, predict diabetes independent of fasting glucose and insulin. sCD36 clusters with important markers of insulin resistance and MetSy that are key predictors of type 2 diabetes.
Handberg A, et al. J Clin Endocrinol Metab. 2010 Apr;95(4):1939-46. Epub 2010 Feb 5.
human soluble CD36 recombinant
Human Soluble CD36 ELISA Kit SK00196-02 had been used in research paper by Dr.  Parra-Rojas I and Ramos-Arellano LE, Laboratorio de Investigación en Obesidad y Diabetes, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, México.
Human Soluble CD36 ELISA
Code No.: SK00196-02
Size: 96 T
Price: $460.00 USD
Standard range: 3.9-250 ng/ml
Sensitivity: 250 pg/ml
Sample Type: EDTA plasma, Serum
Intra-CV: 4-6%
Inter-CV: 8-12%
Protocol: PDF

** human CD36, ECD (E.Coli derived) indicated 20% crossreactivity with SK00196-02
mouse soluble cd36 elisa kit from aviscera bioscience
Mouse Soluble CD36 ELISA
Code No.: SK00196-03
Size: 96 T
Price: $460.00 USD
Standard range: 0.22 -14 ng/ml
Sensitivity: 110 pg/ml
Sample Type: EDTA plasma, Serum
Intra-CV: 4-6%
Inter-CV: 8-12%
Protocol: PDF
human soluble cd36 recombinant from aviscera bioscience
Human Soluble CD36 Rec
Code No.: 00196-01-100
Size: 96 T
Price: $360.00 USD
Protein ID :NP_001001547
Gene ID : 948
Tag; His Tag on N-terminus
Data Sheet: PDF
This protein indicated 20% of crossreactivity with SK00196-02
Mouse Soluble CD36 Rec
Code No.: 00196-04-100
Size: 96 T
Price: $360.00 USD
Protein ID :Q08857
Gene ID : 12491
Sequence: Extracellular Domain
Tag; His Tag on N-terminus
Data Sheet: PDF
This protein may not be detected by SK00196-03
Anti Human soluble CD36 IgG
Code No.: A00196-01-100
Size: 100 ug
Price: $260.00 USD
Host: rabbit
Antigen: human sCD36, rec
Ab Type: Purified IgG
Purification: Protein A
Applications: E, IHC
Working Dilution: 0.1ug/ml)
Data Sheet: PDF
 

 

 

Name
Code No.
Size
Price ($)
Soluble CD36 (Human) ELISA Kit (for plasma and serum samples)
96 T
460.00
Soluble CD36 (Mouse) ELISA Kit
96 T
460.00
Following Soluble CD36 Proteins may not be used as elisa standards for SK00196-02 and or SK00196-03      
CD36 (Human) , Soluble, His Tag. (E. Coli derived)
100 ug
360.00
CD36 (Human) , Soluble, His Tag. (E. Coli derived)
50 ug
250.00
CD36 (Human) , Soluble, His Tag. (E. Coli derived)
00196-01-1000
1 mg
inquire
CD36 (Mouse) , Soluble., His Tag (E. Coli derived)
50 ug
250.00
CD36 (Mouse) , Soluble., His Tag (E. Coli derived)
100 ug
360.00
CD36 (Mouse) , Soluble., His Tag (E. Coli derived)
1 mg
inquire
Soluble CD36 (Human) (HEK293 cells derived)
10 ug
120.00
Soluble CD36 (Human) (HEK293 cells derived)
00196-07-50
50 ug
inquire
Soluble CD36 (Human) (HEK293 cells derived)
00196-07-1000
1 mg
inquire
Soluble CD36 (Rat) (HEK293 cells derived)
10 ug
120.00
Soluble CD36 (Rat) (HEK293 cells derived)
00196-09-50
50 ug
inquire