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Human Cardiotrophin-like Cytokine Factor 1 (CLCF1/CLF1), Recombinant

Alternative name:

  • B-cell-stimulating factor 3 (BSF3)
  • Novel neurotrophin-1 (NNT-1)
  • Gene Name CLF
CLCF1 is a secreted cytokine with B-cell stimulating capability. CLCF1 belongs to IL-6 superfamily. CLCF1 binds to and activates the ILST/gp130 receptor. It predominantly expressed in lymph nodes, spleen, peripheral blood lymphocytes, bone marrow, and fetal liver. CLCF1 and CTNF are two natural ligands for CTNF Receptor. Muation of CLF had been identified on cold-induced sweating syndrome type 2 (CISS2) patients.
Ciliary neurotrophic factor (CNTF) receptor controls a pathway supporting the differentiation and survival of a wide range of neural cell types during development and in adulthood. Cardiotrophin-like cytokine (CLC)-cytokine-like factor 1 (CLF) composite cytokine is a second ligand for the CNTF alpha-component receptor (CNTFRalpha). This composite cytokine is built on the structural model of IL-12, with a complex formed by a four-helix bundle type I cytokine, CLC (also referred to as CLCF1), bound to a soluble receptor subunit, CLF (also known as CRLF1). We have reported mutations in the chaperone soluble receptor CLF, causing cold-induced sweating syndrome (CISS). In this study, we studied the CLC-mutated alleles in a patient suffering from a similar disease. This patient was compound heterozygous for two different CLC mutations. The first allele was inactivated by a stop codon at position 107 (Y107X). In the second allele, a R197L mutation in the CLC-predicted binding site to the CNTFRalpha was detected. Functional analysis of the mutated protein revealed an incapacity for R197L CLC to bind to CNTFRalpha and activate the subsequent signaling events. Structural and docking interaction studies showed that the R197L substitution destabilized the contact site between CLC and CNTFRalpha.
Rousseau F, , et al.Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):10068-73. Epub 2006 Jun 16.

Cross-species functional analysis of cancer-associated fibroblasts identifies a critical role for CLCF1 and IL6 in non-small cell lung cancer in vivo

Cancer-associated fibroblasts (CAFs) have been reported to support tumor progression by a variety of mechanisms. However, their role in the progression of non-small cell lung cancer (NSCLC) remains poorly defined. In addition, the extent to which specific proteins secreted by CAFs contribute directly to tumor growth is unclear. To study the role of CAFs in NSCLC, a cross-species functional characterization of mouse and human lung CAFs was performed. CAFs supported the growth of lung cancer cells in vivo by secretion of soluble factors that directly stimulate the growth of tumor cells. Gene expression analysis comparing normal mouse lung fibroblasts (NFs) and mouse lung CAFs identified multiple genes that correlate with the CAF phenotype. A gene signature of secreted genes upregulated in CAFs was an independent marker of poor survival in NSCLC patients. This secreted gene signature was upregulated in NFs after long-term exposure to tumor cells, demonstrating that NFs are "educated" by tumor cells to acquire a CAF-like phenotype. Functional studies identified important roles for CLCF1-CNTFR and IL6-IL6R signaling, in promoting growth of NSCLC cells. This study identifies novel soluble factors contributing to the CAF protumorigenic phenotype in NSCLC and suggests new avenues for the development of therapeutic strategies.
Vicent S et al. Cancer Res. 2012 Sep 7. [Epub ahead of print]
   
Human Cardiotrophin-like cytokine factor 1 ( CLCF1) ELISA KIt
Code No.: SK00158-01
Size: 96 T
Price: $460.00 USD 
Standard range: 1.56-100 ng/ml
Sensitivity: 50 pg/ml
Sample Type: serum, plasma
Sample require: 100 uL per well
Intra-CV:4-6%
Inter-CV: 8-10% 
Protocol: PDF

human clcf1 recombinant

Human Cardiotrophin-like cytokine factor 1 ( CLCF1) Recombinant
Code No.: 00158-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID:Q9UBD9
Gene ID: 23529
MW:22.2 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 90%
Data Sheet: PDF
Anti Human CLCF1 IgG
Code No.: A00158-01-100
Size: 100 ug
Price: $260.00 USD
Host:Rabbit
Antigen: human CLCF1
Specificity: Human
Antibody Type:Purified IgG
Purification: Protein A affinity
Application: E, IHC
Data Sheet: PDF
More IL-6 superfamily and Adiponectin related Proteins

 

 

elisa kIT
Name
Code No.
Size
Price ($)
Cardiotrophin-like cytokine factor 1 ( CLCF1) (Human) ELISA KIT
96 T
460.00
Cardiotrophin-like cytokine factor 1 ( CLCF1) (Human) , Rec.
00158-01-50
50 ug
220.00
Cardiotrophin-like cytokine factor 1 ( CLCF1) (Human) , Rec.
100 ug
360.00
Cardiotrophin-like cytokine factor 1 ( CLCF1) (Human) , Rec.
00158-01-1000
1000 ug
3200.00
Cardiotrophin-like cytokine factor 1 ( CLCF1) (Human) , Rec. Biotinylated
00158-01-50B
50 ug
460.00
Anti CLCF1 (Human) IgG A00158-01-100 100 ug 260.00
Anti CLCF1 (Human) Monoclonal Antibody A00158-02-100 100 ug 350.00