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CTRP12 ELISA Kits,Recombinant
  • C1q/TNF-related protein 12
  • C1q-like 3

Endopeptidase cleavage generates a functionally distinct isoform of CTRP12 with an altered oligomeric state and signaling specificity

Adipose tissue-derived adipokines are an important class of secreted metabolic regulators that mediate tissue crosstalk to control systemic energy balance. We recently described C1q/TNF-related protein-12 (CTRP12), a novel insulin-sensitizing adipokine that regulates glucose metabolism in liver and adipose tissue. However, the biochemical properties of CTRP12 and its naturally occurring cleaved isoform have not been characterized. Here, we show that CTRP12 is a secreted hormone subjected to multiple functionally relevant posttranslational modifications at highly conserved residues. For example, Asn-39 is glycosylated, while Cys-85 mediates the assembly of higher-order oligomeric structure. Endopeptidase cleavage at Lys-91 generates a cleaved globular gCTRP12 isoform, the expression of which is increased by insulin. PCSK3/furin was identified as the major proprotein convertase expressed by adipocytes that mediates the endogenous cleavage of CTRP12. Cleavage at Lys-91 is context dependent: mutation of the charged Arg-93 to Ala on the P2 position enhanced cleavage, and triple mutations (K90A, K91A, R93A) abolished cleavage. Importantly, the two isoforms of CTRP12 differ in oligomeric structures and are functionally distinct. The full-length protein forms trimers and larger complexes and the cleaved isoform consisted of predominantly dimers. While full-length fCTRP12 strongly activated Akt signaling in H4IIE hepatocytes and 3T3-L1 adipocytes, gCTRP12 preferentially activated MAP kinase (Erk1/2 and p38-MAPK) signaling. Further, only fCTRP12 improved insulin-stimulated glucose uptake in adipocytes. These results reveal a novel mechanism controlling signaling specificity and function of a hormone via cleavage-dependent alteration in oligomeric state.
Wei Z, Lei X, Seldin M, Wong GW. . J Biol Chem. 2012 Aug 31. [Epub ahead of print]
mouse rat ctrp12 elisa kit sk00392-08 enables to detect circulating samples
Mouse/Rat CTRP12 ELISA KIt
Code No.: SK00392-08
Size: 96 T
Price: $460.00 USD 
Standard range: 0.156-20 ng/ml
Sensitivity:50 pg/ml
Sample Type: serum, plasma
Sample require: 100 uL per well
Intra-CV:6-8%
Inter-CV: 8-12% 
Protocol: PDF
human ctrp12 elisa kit from aviscera bioscience
Human CTRP12 ELISA KIt
Code No.: SK00392-06
Size: 96 T
Price: $460.00 USD 
Standard range: 0.049-200 ng/ml
Sensitivity:10 pg/ml
Sample Type: serum, plasma
Sample require: 100 uL per well
Intra-CV:6-8%
Inter-CV: 8-12% 
Protocol: PDF
new human ctrp12 elisa kit sk00392-07 for serum and plasma samples
Human CTRP12 ELISA KIt
Code No.: SK00392-07
Size: 96 T
Price: $460.00 USD 
Standard range: 0.64-2000ng/ml
Linear rage:5 ~ 500 ng/mL
Sensitivity:300 pg/ml
Sample Type: serum, plasma
Sample require: 100 uL per well
Intra-CV:6-8%
Inter-CV: 8-12% 
Protocol: PDF
Human CTRP12/FAM132A Full Length Recombinant
Code No.: 00392-02-100
Size: 100 ug
Price: $390.00 USD
Protein ID:Q5T7M4
Gene ID:388581
MW:47.9 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF

Human CTRP12/FAM132AGlobular Form Recombinant
Code No.: 00392-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID:Q5T7M4
Gene ID:388581
MW:19.5 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
Mouse CTRP12/FAM132A Full Length Recombinant
Code No.: 00392-03-100
Size: 100 ug
Price: $390.00 USD
Protein ID:Q8R2Z0
Gene ID:67389
MW:48.9KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
Mouse CTRP12/FAM132A Globular Form Recombinant
Code No.: 00392-04-100
Size: 100 ug
Price: $360.00 USD
Protein ID:Q8R2Z0
Gene ID:67389
MW:24.8 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
Anti Human CTRP12 IgG
Code No.: A00392-01-100
Size: 100 ug
Price: $260.00 USD
Host:Rabbit
Antigen:Human CTRP12
Specificity:Human
Antibody Type:Purified IgG
Purification: Protein A affinity
Application: E, IHC
Data Sheet: PDF
Anti Mouse CTRP12 IgG
Code No.: A00392-03-100
Size: 100 ug
Price: $260.00 USD
Host:Rabbit
Antigen:Mouse CTRP12
Specificity: Mouse
Antibody Type:Purified IgG
Purification: Protein A affinity
Application: E, IHC
Data Sheet: PDF
Name
Code No.
Size
Price ($)
CTRP12 (Mouse/Rat) ELISA Kit
96 T
460.00
CTRP12 (Human) ELISA Kit
96 T
460.00
CTRP12 (Human) ELISA Kit
96 T
460.00
CTRP12 /FAM132A (Human) Rec.
100 ug
390.00
CTRP12/FAM132A Globular Form (Mouse) Rec.
100 ug
360.00
CTRP12 Full Length (Mouse) Rec.
100 ug
390.00
CTRP12 Globular Form (Mouse), Rec.
100 ug
360.00
Anti CTRP12 (Human) IgG
100 ug
260.00
Anti CTRP12 (Human) IgG biotinylated
50 ug
300.00
Anti CTRP12 (Mouse) IgG
100 ug
260.00
Anti CTRP12 (Mouse) IgG biotinylated
50 ug
300.00
Anti CTRP12 (Mouse) IgG
100 ug
260.00
Anti CTRP12 (Mouse) IgG biotinylated
50 ug
300.00