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Delta Like Protein1 (DLL1) ELISA Kit-Delta Like Protein 4 (DLL4) ELISA Kit

Alternative name:

  • DLL1
  • Delta 1
  • DLL4
  • Delta 4 

Notch ligand delta-like 4 blockade attenuates atherosclerosis and metabolic disorders

Atherosclerosis and insulin resistance are major components of the cardiometabolic syndrome, a global health threat associated with a systemic inflammatory state. Notch signaling regulates tissue development and participates in innate and adaptive immunity in adults. The role of Notch signaling in cardiometabolic inflammation, however, remains obscure. We noted that a high-fat, high-cholesterol diet increased expression of the Notch ligand Delta-like 4 (Dll4) in atheromata and fat tissue in LDL-receptor-deficient mice. Blockade of Dll4-Notch signaling using neutralizing anti-Dll4 antibody attenuated the development of atherosclerosis, diminished plaque calcification, improved insulin resistance, and decreased fat accumulation. These changes were accompanied by decreased macrophage accumulation, diminished expression of monocyte chemoattractant protein-1 (MCP-1), and lower levels of nuclear factor-κB (NF-κB) activation. In vitro cell culture experiments revealed that Dll4-mediated Notch signaling increases MCP-1 expression via NF-κB, providing a possible mechanism for in vivo effects. Furthermore, Dll4 skewed macrophages toward a proinflammatory phenotype ("M1"). These results suggest that Dll4-Notch signaling plays a central role in the shared mechanism for the pathogenesis of cardiometabolic disorders.
Fukuda D et al. Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):E1868-77

Expanding role of delta-like 4 mediated notch signaling in cardiovascular and metabolic diseases

Cardiometabolic disease, a global health threat, has been linked to chronic inflammation, in which activated macrophages play a key role. Macrophages are highly heterogeneous hematopoietic cells found in nearly every tissue in the body. Various stimuli recruit monocytes into the cardiovascular system and metabolic organs, where they differentiate to macrophages, and activate these pro-inflammatory phagocytes, leading to the initiation and development of inflammation in these organs. Key regulators of macrophage activation therefore may serve as therapeutic targets for cardiometabolic disease. The Notch signaling pathway, involving 5 ligands and 4 receptors, regulates the differentiation of various cell types during development, and also contributes to the disease processes in adults. We found that the Notch ligand delta-like 4 (Dll4) activates macrophages in vitro as determined by the induction of genes and pathways associated with cardiovascular and metabolic disorders. Our recent study demonstrated in vivo that blockade of Dll4 by a neutralizing antibody attenuates key features typical of cardiovascular and metabolic diseases, such as accumulation of activated macrophages in arteries and fat; chronic atherosclerosis; arterial and valvular calcification; insulin resistance; and fatty liver. These results suggest that Dll4-mediated Notch signaling participates in the shared disease mechanisms for cardiovascular and metabolic disorders. This review summarizes the role of macrophages and Dll4/Notch signaling in the development of inflammation in both the cardiovascular system and metabolic organs. 
Fukuda D, Aikawa M. Circ J. 2013;77(10):2462-8. Epub 2013 Sep 12.

Synaptojanin-2 Binding Protein Stabilizes the Notch Ligands DLL1 and DLL4 and Inhibits Sprouting Angiogenesis

RATIONALE: The formation of novel blood vessels is initiated by vascular endothelial growth factor. Subsequently, DLL4-Notch signaling controls the selection of tip cells, which guide new sprouts, and trailing stalk cells. Notch signaling in stalk cells is induced by DLL4 on the tip cells. Moreover,DLL4 and DLL1 are expressed in the stalk cell plexus to maintain Notch signaling. Notch loss-of-function causes formation of a hyperdense vascular network with disturbed blood flow.
OBJECTIVE: This study was aimed at identifying novel modifiers of Notch signaling that interact with the intracellular domains of DLL1 and DLL4.
METHODS AND RESULTS:Synaptojanin-2 binding protein (SYNJ2BP, also known as ARIP2) interacted with the PDZ binding motif of DLL1 andDLL4, but not with the Notch ligand Jagged-1. SYNJ2BP was preferentially expressed in stalk cells, enhanced DLL1 and DLL4 protein stability, and promoted Notch signaling in endothelial cells. SYNJ2BP induced expression of the Notch target genes HEY1, lunatic fringe (LFNG), and ephrin-B2, reduced phosphorylation of ERK1/2, and decreased expression of the angiogenic factor vascular endothelial growth factor (VEGF)-C. It inhibited the expression of genes enriched in tip cells, such as angiopoietin-2, ESM1, and Apelin, and impaired tip cell formation. SYNJ2BP inhibited endothelial cell migration, proliferation, and VEGF-induced angiogenesis. This could be rescued by blockade of Notch signaling or application of angiopoietin-2. SYNJ2BP-silenced human endothelial cells formed a functional vascular network in immunocompromised mice with significantly increased vascular density.
CONCLUSIONS:These data identify SYNJ2BP as a novel inhibitor of tip cell formation, executing its functions predominately by promoting Delta-Notch signaling.
Adam MG et al. Circ Res. 2013 Nov 8;113(11):1206-18. doi: 10.1161/CIRCRESAHA.113.301686. Epub 2013 Sep 11
human dll1 elisa kit from aviscera bioscience Human Delta Like Protein 1 (DLL1) ELISA
Code No.:SK00537-01
Size: 96 T
Price: $420.00 USD
Standard range: 46- 3000 pg/ml
Sample type: serum, plasma, urine
Sample require: 100 uL per well
Specificity: human DLL1
Intra-CV:4-6%
Inter-CV: 8-12%
Protocol: PDF
Notice: Research use only
human dll4 elisa kit from aviscera bioscience Human Delta Like Protein 4 (DLL4) ELISA
Code No.:SK00538-01
Size: 96 T
Price: $420.00 USD
Standard range: 31- 2000 pg/ml
Sample type: Cell Cultures
Sample require: 100 uL per well
Specificity: human DLL4
Intra-CV:4-6%
Inter-CV: 8-12%
Protocol: PDF
Notice: Research use only
  Mouse Delta Like Protein 4 (DLL4) ELISA
Code No.:SK00538-03
Size: 96 T
Price: $420.00 USD
Standard range: 9.375- 600 pg/ml
Sample type: serum, plasma, urine
Sample require: 100 uL per well
Specificity: mouse DLL4
Intra-CV:4-6%
Inter-CV: 8-12%
Protocol: PDF
Notice: Research use only

 

 

Name
Code No.
Size
Price ($)
Human Delta Like Protein 1 (DLL1) ELISA
96 T
420.00
Human Delta Like Protein 4 (DLL4) ELISA
96 T
420.00
Mouse DLL4 ELISA Kit
96 T
420.00
Human Delta Like Protein 4 (DLL4) (HEK293 cell)
00538-02-50
50 ug
400.00
Human Delta Like Protein 4 (DLL4) (HEK293 cell)
00538-02-1000
1 mg
4000
Human Delta Like Protein 4 (DLL4) Fc (HEK293 cell)
00538-05-50
50 ug
400.00
Human Delta Like Protein 4 (DLL4) Fc (HEK293 cell)
00538-05-1000
1 mg
4000.00
Mouse Delta Like Protein 4 (DLL4) (HEK293 cell)
00538-04-50
50 ug
400.00
       
       
       

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