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Decorin ELISA

Alternative name:

  • small leucine-rich protein 1B
  • dermatan sulphate proteoglycans II
  • Bone proteoglycan II
  • proteoglycan core protein
  • Gene name DCN/PGSII
The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of
various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome.

Decorin Is a Novel VEGFR-2-Binding Antagonist for the Human Extravillous Trophoblast

Extravillous trophoblasts (EVT) of the human placenta invade the uterine decidua and its arteries to ensure successful placentation. We previously identified two decidua-derived molecules, TGF-β and a TGF-β-binding proteoglycan decorin (DCN), as negative regulators of EVT proliferation, migration, and invasiveness and reported that DCN acts via multiple tyrosine kinase receptors [epidermal growth factor-receptor (EGF-R), IGF receptor-1 (IGFR1), and vascular endothelial growth factor 2 receptor (VEGFR-2)]. Because binding of DCN to VEGFR-2 has never been reported earlier, present study explored this binding, the approximate location of VEGFR-2-binding site in DCN, and its functional role in our human first trimester EVT cell line HTR-8/SVneo. Based on far-Western blotting and coimmunoprecipitation studies, we report that DCN binds both native (EVT expressed) and recombinant VEGFR-2 and that this binding is abrogated with a VEGFR-2 blocking antibody, indicating an overlap between the ligand-binding and the DCN-binding domains of VEGFR-2. We determined that 125I-labeled VEGF-E (a VEGFR-2 specific ligand) binds EVT with a dissociation constant (Kd) of 566 pM, and DCN displaced this binding with an inhibition constant (Ki) of 3.93–5.78 nM, indicating a 7- to 10-fold lower affinity of DCN for VEGFR-2. DCN peptide fragments derived from the leucine rich repeat 5 domain that blocked DCN-VEGFR-2 interactions or VEGF-E binding in EVT cells also blocked VEGF-A- and VEGF-E-induced EVT cell proliferation and migration, indicative of functional VEGFR-2-binding sites of DCN. Finally, DCN inhibited VEGF-E-induced EVT migration by interfering with ERK1/2 activation. Our findings reveal a novel role of DCN as an antagonistic ligand for VEGFR-2, having implications for pathophysiology of preeclampsia, a trophoblast hypoinvasive disorder in pregnancy, and explain its antiangiogenic function.

Gausal A. Khan, et al. June 9, 2011 Molecular Endocrinology June 9, 2011 me.2010-0426

Human Decorin ELISA
Code No.: SK00641-01
Size: 96 T
Price: $360.00 USD
Standard Range:15.6-1000 pg/ml
Sensitivity:7.8 pg/ml
Sample Type: EDTA plasma
Sample requres: 100uL per well
IntraCV: 6-8%
InterCV: 10-12%
Protocol: PDF
anti human decorin antibody
Anti Human Decorin IgG
Code No.: A00641-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Antigen: human Decorin Rec.
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: E, IHC, WB
Working Dilution: 2 ug/ml)
Data Sheet: PDF

 

Name
Code No.
Size
Price ($)
Decorin (Human) ELISA Kit
96 T
360.00
Decorin (Human) recombinant
100 ug
360.00
Anti Decorin (Human) IgG
100 ug
260.00
Anti Decorin (Human) IgG Biotinylated
50 ug
340.00
Anti Decorin (Human) IgG FITC conjugated
A00641-01-50F
50 ug
340.00
Anti Decorin (Human) IgG Cy3 conjugated
A00641-01-50C3
50 ug
420.00
Anti Decorin (Human) Monoclonal Antibody
100 ug
260.00
Anti Decorin (Human) Monoclonal Antibody Biotinylated
50 ug
350.00
Anti Decorin (Human) Monoclonal Antibody
100 ug
260.00
Anti Decorin (Human) Monoclonal Antibody Biotinylated
50 ug
350.00