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Epigen (EPGN) (Soluble) ELISA Kit

Alternative name:

  • Epithelial mitogen (EPG)
  • Mitogenic ligand of the EGFR
Epigen romotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.
The levels of expression of the four receptors and eleven ligands composing the epidermal growth factor family were measured using immunohistochemical staining in one hundred cases of breast cancer. All of the family were expressed to some degree in some cases; however, individual cases showed a very wide range of expression of the family from essentially none to all the factors at high levels. The highest aggregate level of expression of a receptor was HER2 followed by HER1, then HER3, then HER4. The ligands (including two splice variants of the NRG1 and NRG2 genes) broadly fell into three groups, those with the highest aggregate expression were Epigen, Epiregulin, Neuregulin 1alpha, Neuregulin 2alpha, Neuregulin 2beta, Neuregulin 4 and TGFalpha, moderate expression was seen with EGF, Neuregulin 1beta and Neuregulin 3, and relatively low levels of expression were seen of HB-EGF, Betacellulin and Amphiregulin. Statistical analysis using Spearman's Rank Correlation showed a positive correlation of expression between each of the factors. Analysing the data using the Cox Proportional Hazards model showed that, in this dataset, the most powerful predictors of relapse free interval and overall survival were the combined measurement of only Epigen and Neuregulin 4.
McIntyre E, et al. Breast Cancer Res Treat. 2010 Jul;122(1):105-10. Epub 2009 Sep 17.

Ectodomain shedding of the EGF-receptor ligand epigen is mediated by ADAM17

All ligands of the epidermal growth factor receptor (EGFR), which has important roles in development and disease, are made as transmembrane precursors. Proteolytic processing by ADAMs (a disintegrin and metalloprotease) regulates the bioavailability of several EGFR-ligands, yet little is known about the enzyme responsible for processing the recently identified EGFR ligand, epigen. Here we show that ectodomain shedding of epigenrequires ADAM17, which can be stimulated by phorbol esters, phosphatase inhibitors and calcium influx. These results suggest that ADAM17 might be a good target to block the release of bioactive epigen, a highly mitogenic ligand of the EGFR which has been implicated in wound healing and cancer.
FEBS Lett. 2007 Jan 9;581(1):41-4. Epub 2006 Dec 6.
 
 
human epigen elisa kit |aviscera bioscience
Human Epigen/Epithelial Mitogen (EPGN)ELISA
Code No.: SK00340-01
Size: 96 T
Price: $420.00 USD
Standard Range:31-2000 pg/ml
Sensitivity:10 pg/ml
Sample Type: cell culture, serum, EDTA plasma
Sample requres: 100uL per well
IntraCV: 6-8%
InterCV: 10-12%
Protocol: PDF

human epigen recombinant 00340-01-100

Human Epigen Recombinant
Code No.: 00340-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID:Q6UW88
Gene ID: 255324
MW:8.7 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
anti human epigen antibody from aviscera bioscience Anti Human Soluble Epigen IgG
Code No.: A00340-01-100
Size: 100 ug
Price: $260.00 USD
Host:Rabbit
Antigen: human Epigen
Antibody Type:Purified IgG
Purification: Protein A affinity
Application: E, IHC, WB
Data Sheet: PDF
Anti Human Soluble Epigen IgG
Code No.: A00340-01-100
Size: 100 ug
Price: $260.00 USD
Host:Rabbit
Antigen: human Epigen
Antibody Type:Purified IgG
Purification: Protein A affinity
Application: E, IHC, WB
Data Sheet: PDF

 

Name
Code No.
Size
Price ($)
Soluble Epigen (EPGN) (Human) ELISA Kit
96 T
420.00
Epigen Extracellular Domain (Human) Rec.
00340-01-50
50 ug
180.00
Epigen Extracellular Domain (Human) Rec.
100 ug
240.00
Epigen Extracellular Domain (Human) Rec.
00340-01-1000
1000 ug
1800.00
Epigen Extracellular Domain (Human) Rec. Biotinylated
00340-01-50B
50 ug
360.00
Anti Soluble Epigen (Human) IgG A00340-01-100 100 ug 260.00
Anti Soluble Epigen (Human) IgG Biotinylated A00340-01-50B 50 ug 290.00
Anti Soluble Epigen (Human) Monoclonal Antibody A00340-02-100 100 ug 260.00
Anti Soluble Epigen (Human) Monoclonal Antibody A00340-03-100 100 ug 260.00
Anti Soluble Epigen (Human) Monoclonal Antibody A00340-04-100 100 ug 260.00
1: Dahlhoff M, Müller AK, Wolf E, Werner S, Schneider MR. Epigen transgenic mice  develop enlarged sebaceous glands. J Invest Dermatol. 2010 Feb;130(2):623-6. Epub 2009 Aug 20.
2: Sahin U, Blobel CP. Ectodomain shedding of the EGF-receptor ligand epigen is mediated by ADAM17. FEBS Lett. 2007 Jan 9;581(1):41-4. Epub 2006 Dec 6.
3: Kochupurakkal BS, Harari D, Di-Segni A, Maik-Rachline G, Lyass L, Gur G, Kerber G, Citri A, Lavi S, Eilam R, Chalifa-Caspi V, Eshhar Z, Pikarsky E, Pinkas-Kramarski R, Bacus SS, Yarden Y. Epigen, the last ligand of ErbB
receptors, reveals intricate relationships between affinity and mitogenicity. J Biol Chem. 2005 Mar 4;280(9):8503-12. Epub 2004 Dec 17.
4: Strachan L, Murison JG, Prestidge RL, Sleeman MA, Watson JD, Kumble KD. Cloning and biological activity of epigen, a novel member of the epidermal growth factor superfamily. J Biol Chem. 2001 May 25;276(21):18265-71. Epub 2001 Jan 16.