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Endothelial cell-Specific Molecule 1(ESM1)/Endocan (Human) ELISA, Endocan (Mouse) ELISA

Alternative name:

  • endocan
  • endothelial-cell specific molecule-1
ESM1 is a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene. ESM1 also is secreted adipokine. Circulating ESM-1 levels increased significantly, albeit modestly. ESM-1 is actively produced by adipocytes. However, since ESM-1 adipocyte gene expression and circulating plasma levels are not correlated, other sources of ESM-1 may be more important. Circulating ESM-1 levels are reduced in the overweight and obese, consistent with the notion that ESM-1 may play some role in obesity-associated vascular disease.
Human ESM1/Endocan ELISA Kit SK00318-06 had been used by Dr. Jing YY on following research paper.
Serum endocan levels are correlated with the presence and severity of coronary artery disease in patients with hypertension.
BACKGROUND: Endothelial dysfunction is one of the most important early indicators of atherosclerosis in hypertension (HT) patients. Endocan has been reported to play a role in the pathophysiology of endothelial dysfunction. OBJECTIVE: We sought to assess whether serum endocan levels are correlated with the presence and severity of coronary artery disease (CAD) in patients with HT. METHODS:We measured endocan levels in 164 patients with HT and in 55 controls. The severity of CAD was assessed by the coronary atherosclerosis index scores. RESULTS: Serum endocan levels were independently correlated with the presence and severity of CAD in HT patients. CONCLUSION:Endocan might function as a useful biomarker for monitoring the development and progression of CAD in HT patients.
Wang XS, Yang W, Luo T, Wang JM, Jing YY. Genet Test Mol Biomarkers. 2015 Mar;19(3):124-7. doi: 10.1089/gtmb.2014.0274. Epub 2015 Jan 14.
Adipose tissue and circulating endothelial cell specific molecule-1 in human obesity
Adipocytes produce the endothelial-cell specific molecule-1 (ESM-1), which inhibits leukocyte adhesion and migration through the endothelium. This study investigates ESM-1 expression and regulation in human adipose tissue. Subcutaneous abdominal adipose tissue was obtained from seventy postmenopausal women. Fourteen women subsequently underwent non-pharmacological weight reduction. In vitro experiments were performed on adipocytes isolated from human mammary adipose tissue. We determined gene expression by TaqMan RT-PCR and measured ESM-1 levels in serum and cell culture medium by ELISA. Mature adipocytes produced ESM-1. ESM-1 gene expression was higher in adipocytes than in preadipocytes. Cortisol inhibited ESM-1 gene expression in preadipocytes. Insulin and cortisol inhibited adipocyte ESM-1 production in adipocytes. This inhibitory effect of insulin was attenuated by insulin resistance, as ESM-1 gene expression in subcutaneous adipose tissue was increased in obese, hyperinsulinemic women. In contrast, ESM-1 serum levels were reduced in obese women and inversely correlated to C-reactive protein levels. Five percent weight loss did not markedly change gene expression. Circulating ESM-1 levels increased significantly, albeit modestly. ESM-1 is actively produced by adipocytes. However, since ESM-1 adipocyte gene expression and circulating plasma levels are not correlated, other sources of ESM-1 may be more important. Circulating ESM-1 levels are reduced in the overweight and obese, consistent with the notion that ESM-1 may play some role in obesity-associated vascular disease.
Endothelial cell phenotypes are differentially regulated between different sites of the vascular tree. We tested the hypothesis that endocan, a novel soluble dermatan sulfate proteoglycan, is differentially expressed in the intact endothelium and that site-specific expression is mediated by signals in the local microenvironment. Using a combination of Northern blot analyses, Taqman RT-PCR, and in situ hybridizations, endocan was shown to be preferentially expressed in the endothelial lining of tumor xenografts, including human non-small cell lung cancer, rat glioma, and human renal cell carcinoma. In contrast, endocan mRNA was expressed at low levels in embryos between E4.5 and E18.5. Under in vitro conditions, endocan expression in human umbilical vein endothelial cells (HUVEC) was upregulated by tumor cell-conditioned medium, an effect that was inhibited by the addition of neutralizing antibody to vascular endothelial growth factor (VEGF). Moreover, treatment of HUVEC with VEGF resulted in a dose- and time-dependent increase in endocan mRNA. The results suggest that endocan is preferentially expressed in tumor endothelium in vivo and that its expression is regulated by tumor-derived factors.
Abid MR, et al. .Microvasc Res. 2006 Nov;72(3):136-45. Epub 2006 Sep 7.
Endothelial cell specific molecule-1--a newly identified protein in adipocytes
Expression of the endothelial cell-specific molecule (ESM)-1 was originally identified in lung and kidney endothelial cells, where its expression is regulated by cytokines. In vitro, ESM-1 interferes with the molecular mechanisms of immune cell migration by binding to adhesion molecules. In this study, we have explored the expression of ESM-1 in isolated human adipocytes and in rat adipose tissue depots. Human primary adipocytes were cultivated after collagenase digestion and used for in vitro incubation studies. Adipocytes were also isolated from different fat depots of Sprague-Dawley rats. Gene expression was quantified by TaqMan RT-PCR using specific human and rat ESM-1 primers. The cellular localisation of ESM-1 was determined by confocal microscopy using a specific antibody. ESM-1 expression in human adipocytes was stimulated by phorbol ester, an activator of protein kinase C, and by retinoic acid, an activator of nuclear receptors. The maximum increase in gene expression was 3.2-fold after 72 h treatment with phorbol ester and 4.6-fold after 72 h treatment with retinoic acid. The highest expression was found in subcutaneous rat adipose tissue - two-fold compared to epididymal and six-fold compared to intrascapular brown adipose tissue. As obesity is related to systemic inflammation (examplified by increased circulating levels of C-reactive protein and interleukin-6), the formation of ESM-1 in adipocytes and its activation by protein kinase C may play a role in the regulation of inflammatory processes.
Wellner M, et al. Horm Metab Res. 2003 Apr;35(4):217-21.

New human ESM1 /Endocan ELISA Kit for serum and plasma samples assay

human ESM1 ELISA Kit SK00318-06 enables to detect human  ESM1 on human circulating samples fom aviscera bioscience
   
human endocan /esm1 elisa kit enables to measure human serum and plasma samples sk00318-06 aviscera bioscience Human ESM-1 /Endocan ELISA
Code No.: SK00318-06
Size: 96 T
Price: $460.00 USD
Standard Range:3.125-200 ng/ml
Sensitivity:  500 pg/ml
Sample Type: serum, EDTA plasma
Sample requres: pretreatment of samples
IntraCV: 6-8%
InterCV: 10-12%
Protocol: PDF
Mouse ESM-1 /Endocan ELISA Kit
Code No.: SK00318-19
Size: 96 T
Price: $460.00 USD
Standard Range:78-2500 pg/ml
Sensitivity:  30 pg/ml
Specificity: Mouse ESM1/Endocan (HEK293 cells derived 50 KD). BALB/C mouse serum can not be detected .
Calibration: Mouse ESM1 derived from HEK293 (50KD)
Sample Type: Serum,Urine, Cell cultures.
Sample requres: 50 uL per well
IntraCV: 6-8%
InterCV: 10-12%
Protocol: PDF
  Mouse ESM-1 /Endocan ELISA Kit
Code No.: SK00318-04
Size: 96 T
Price: $460.00 USD
Standard Range:312 -10000 pg/ml
Sensitivity:  150 pg/ml
Specificity: Mouse ESM1/Endocan (HEK293 cells derived 50 KD). BALB/C mouse serum can not be detected .
Calibration: Mouse ESM1 derived from HEK293 (50KD)
Sample Type: Serum,Urine, Cell cultures.
Sample requres: 50 uL per well
IntraCV: 6-8%
InterCV: 10-12%
Protocol: PDF
Mouse ESM-1 ELISA
Code No.: SK00318-09
Size: 96 T
Price: $420.00 USD
Standard Range:3.12 - 50 ng/ml
Sensitivity:  1.8 ng/ml
Calibration: Mouse ESM1 derived from HEK293 cells (50 KD)
Specificity: Mouse ESM1 derived from HEK293 (50 KD)
BALB/C Mouse Serum can not be detected.
Sample Type: HEK293 cell cultures
Sample requres: 50 uL per well
IntraCV: 6-8%
InterCV: 10-12%
Protocol: PDF
   
anti human endocan monoclonal antibody validated
Anti Human Endocan/ESM1 Monoclonal Antibody
Code No.: A00318-09-100
Size: 100 ug
Price: $360.00 USD
Host: Mice
Antigen: human ESM1 Rec.
Ab Type: Monoclonal
Purification: Protein G
Clone: 2B1F3G10
Applications: E, WB
Working Dilution: WB: 2-4 ug/ml
Data Sheet:PDF
Anti Human ESM1 IgG
Code No.: A00318-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Antigen: human ESM1Rec.
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: E, IHC, WB
Working Dilution: 2 ug/ml)
Data Sheet: PDF
human ESM1 recombinant is available in adipobioscience
Human ESM1 Recombinant
Code No.: 00318-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID:Q9NQ30
Gene ID: 11082
MW:20 KD (monomer), 40 KD (dimer)
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 80%
Data Sheet: PDF
Anti Endocan/ESM1 (Mouse) IgG
Code No.: A00318-10-100
Size: 100 ug
Price: $360.00 USD
Target Protein ID:Q9NQ30
Host: rabbit
Antigen: recombinant mouse endocan
Purification: Protein A
Application: IHC, E, WB
WB:24 KD (cell cultures), 56 KD (serum samples)
Data Sheet: PDF

 

Name
Code No.
Size
Price ($)
Endothelial-cell Specific Molecule-1 (ESM-1) /Endocan (Human) ELISA Kit for serum or plasma SK00318-06 96 T 460.00
ESM-1/Endocan (Mouse) ELISA Kit SK00318-19 96 T 460.00
ESM-1/Endocan (Mouse) ELISA Kit SK00318-04 96 T 460.00
ESM-1/Endocan (Mouse) ELISA Kit SK00318-09 96 T 460.00
ESM-1/Endocan (Mouse) ELISA Kit SK00318-03 96 T inquire
Endocan / ESM1 (Human) (Human cells derived) recombinant
00318-11-05
5 ug
260.00
Endocan / ESM1 (Mouse) (Human cells derived) recombinant
00318-12-05
5 ug
260.00
ESM1 (Human) recombinant
00318-01-50
50 ug
250.00
ESM1 (Human) recombinant
100 ug
360.00
Endocan / ESM1 (Mouse) recombinant
00318-12-50
50 ug
250.00
Endocan / ESM1 (Mouse) recombinant
00318-12-100
100 ug
360.00
Rabbit Anti ESM1 (Human) IgG
100 ug
220.00
Anti ESM1 (Human) Monoclonal IgG
100 ug
260.00
Anti ESM1 (Human) Monoclonal IgG
100 ug
260.00
Anti ESM1 (Human) Monoclonal IgG
100 ug
260.00
Anti ESM1 (Human) Monoclonal IgG
100 ug
260.00
Anti ESM1 (Human) Monoclonal IgG
100 ug
260.00
Anti ESM1 (Mouse) IgG
100 ug
260.00
Anti ESM1 (Mouse) IgG
100 ug
320.00