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Elastin (Soluble) ELISA Kit
Soluble Elastin is a Biomarker for Stabilization of Atherosclerotic Plaque of Atherosclerosis

Soluble elastin decreases in the progress of atheroma formation in human aorta. Akima T et al. Circ J. 2009 Nov;73(11):2154-62

The anti-inflammatory effects of exercise training promote atherosclerotic plaque stabilization in apolipoprotein E knockout mice with diabetic atherosclerosis

Physical exercise is the cornerstone of cardiovascular disease treatment. The present study investigated whether exercise training affectsatherosclerotic plaque composition through the modification of inflammatory-related pathways in apolipoprotein E knockout (apoE(-/-)) mice with diabetic atherosclerosis. Forty-five male apoE(-/-) mice were randomized into three equivalent (n=15) groups: control (CO), sedentary (SED), and exercise (EX). Diabetes was induced by streptozotocin administration. High-fat diet was administered to all groups for 12 weeks. Afterwards, CO mice were euthanatized, while the sedentary and exercise groups continued high-fat diet for 6 additional weeks. Exercising mice followed an exercise program on motorized-treadmill (5 times/week, 60 min/session). Then, blood samples and atherosclerotic plaques in the aortic root were examined. A considerable (P<0.001) regression of the atherosclerotic lesions was observed in the exercise group (180.339 ± 75.613 x10(3)μm(2)) compared to the control (325.485 ± 72.302 x10(3)μm(2)) and sedentary (340.188 ± 159.108 x 10(3)μm(2)) groups. We found decreased macrophages, matrix metalloproteinase-2 (MMP-2), MMP-3, MMP-8 and interleukin-6 (IL-6) concentrations (P<0.05) in the atherosclerotic plaques of the exercise group. Compared to both control and sedentary groups, exercise training significantly increased collagen (P<0.05), elastin (P<0.001), and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) (P<0.001) content in the atherosclerotic plaques. Those effects paralleled with increased fibrous cap thickness and less internal elastic lamina ruptures after exercise training (P<0.05), while body-weight and lipid parameters did not significantly change. Plasma MMP-2 and MMP-3 concentrations in atherosclerotic tissues followed a similar trend. From our study we can conclude that exercise training reduces and stabilizes atherosclerotic lesions in apoE-/- mice with diabetic atherosclerosis. A favorable modification of the inflammatory regulators seems to explain those beneficial effects.
Kadoglou NP et al. Eur J Histochem. 2013 Mar 25;57(1)
human soluble elastin elisa kit sk00806-01 enables to test elastin on human serum samples. That is available in aviscera bioscince
 
human soluble elastin elisa kit sk00806-01 enables to test human soluble elastin on serum samples
Human Elastin (Soluble) ELISA Kit
Code No.: SK00806-01
Size: 96 T
Price: $460.00 USD
Standard Range:0.64-2000 ng/ml
Sample Type: serum
Sample require: 50 uL per well
Intra-CV: 4-6%
Inter-CV: 8-10%
Protocol: PDF

 

Name
Code No.
Size
Price ($)
Elastin (Soluble) (Human) ELISA Kit
96 T
460.00