AVISCERA BIOSCIENCE  
line decor
  HOME  ::  
line decor
   
 
Human FGF-19 ELISA KIT
FGF19 play key roles in enterohepatic signaling, regulation of liver bile acid biosynthesis, gallbladder motility and metabolic homeostasis.
Jones SA. Adv Exp Med Biol. 2012;728:171-82. doi: 10.1007/978-1-4614-0887-1_11.

Fibroblast growth factor-19 action in the brain reduces food intake and body weight and improves glucose tolerance in male rats

Fibroblast growth factor-19 (FGF19) and its rodent ortholog, FGF15, are hormones produced in the distal small intestine and secreted into the circulation after a meal. In addition to controlling the enterohepatic circulation of bile acids, FGF15/19 also regulates systemic lipid and glucose metabolism. In these experiments we investigated the hypothesis that, like other gut-derived postprandial hormones, FGF15/19 can act in the central nervous system to elicit its metabolic effects. We found that FGF-receptors 1 and 4 are present in rat hypothalamus, and that their expression was reduced by up to 60% in high-fat fed rats relative to lean controls. Consistent with a potential role for brain FGF15/19 signaling to regulate energy and glucose homeostasis, and with a previous report that intracerebroventricular (i.c.v.) administration of FGF19 increases energy expenditure, we report that acute i.c.v. FGF19 reduces 24-h food intake and body weight, and acutely improves glucose tolerance. Conversely, i.c.v. administration of an FGF-receptor inhibitor increases food intake and impairs glucose tolerance, suggesting a physiological role for brain FGF receptor signaling. Together, these findings identify the central nervous system as a potentially important target for the beneficial effects of FGF19 in the treatment of obesity and diabetes.
Ryan KK, et al. Endocrinology. 2013 Jan;154(1):9-15. doi: 10.1210/en.2012-1891. Epub 2012 Nov 26.

 

Fibroblast Growth Factor 19 and 7α-Hydroxy-4-Cholesten-3-one in the Diagnosis of Patients With Possible Bile Acid Diarrhea

OBJECTIVES: Increased colonic bile acids can cause chronic diarrhea. Bile acid diarrhea (BAD) is treatable by sequestrants, and may be secondary to ileal disease or primary BAD. It is underdiagnosed, partly because the selenium-75-homocholic acid taurine (SeHCAT) retention test is not available in many countries, and is underutilized in others. Serum 7α-hydroxy-4-cholesten-3-one (C4), a measure of bile acid synthesis, is available for diagnosis in specialist centers. Recently, deficiency of the ileal hormone fibroblast growth factor 19 (FGF19) has been shown in BAD. Our aim is to evaluate the diagnostic value of FGF19 in a large and prospective group of patients with chronic diarrhea, previously investigated with C4.
METHODS: Patients undergoing routine investigation provided fasting blood samples. C4 was determined by high-performance liquid chromatography, and used to stratify two groups: group 1 (n=119), consisting of patients with normal C4 (= 28?ng/ml), and group 2 (n=139), consisting of patients with high C4 (>28?ng/ml), including any of the possible causes of BAD. Serum FGF19 was measured in stored samples by enzyme-linked immunosorbent assay.
FGF19 and C4 were significantly inversely related (r(s)=-0.64, P<0.001). Patients with raised C4 had significantly lower median FGF19 values. Both of these were more marked when secondary to ileal disease, in particular ileal resection, than in primary BAD. The sensitivity and specificity of FGF19 at 145?pg/ml for detecting a C4 level >28?ng/ml were 58% and 79%, respectively. For C4 >60?ng/ml, these were 74% and 72%; on receiver-operating characteristic analysis, the area under the curve was 0.80 (95% confidence interval 0.74-0.87).
CONCLUSIONS: Serum FGF19 could be developed as a simple blood test to increase the diagnostic rates of BAD.
Pattni SS, et al. Clin Transl Gastroenterol. 2012 Jul 26;3:e18. doi: 10.1038/ctg.2012.10.
FGf19 ELISA Kit
 
  Human FGF19 Ultrsensitive ELISA Kit
Code No.: SK00148-08
Size: 96 T
Price: $490.00 USD
Standard Range:15.6-1000 pg/ml
Sample Type:serum, EDTA plasma
Sample Volume: 100 uL
reading Ex/Em=325/420nm:
Intra CV: 4-6%
Inter CV: 8-10%
Protocol: PDF
Human FGF19 ELISA
Code No.: SK00148-06
Size: 96 T
Price: $460.00 USD
Standard Range:62.5-4000 pg/ml
Sample Type:serum, EDTA plasma
Sample Volume: 100 uL
Dilution Factor:
Intra CV: 4-6%
Inter CV: 8-10%
Protocol: PDF

 

Human FGF19 ELISA
Code No.: SK00148-01
Size: 2 X 96 T
Price: $720.00 USD
Standard Range:39-2500 pg/ml
Sample Type:serum, EDTA plasma
Sample Volume: 100 uL
Dilution Factor:
Intra CV: 4-6%
Inter CV: 8-10%
Protocol: PDF
   

Human FGF19 Recombinant
Code No.:00148-01-100
Size: 100 ug
Price: $360.00 USD
Gene ID: NM_005117
Protein ID: NP_005108
Sequence: Mature form
MW: 22KD
Tag: His tag
Source: E. Coli expressed
Purity: 95% in SDS-PAGE Gel
Formulation: PBS lyophilized
Storage: -20 oC ~ -70oC
Reconstitution: 100 uL
Data Sheet: PDF

mouse FGF21 recombinant

Human FGF19-21 Recombinant
Code No.:00145-08-100
Size: 100 ug
Price: $580.00 USD
Sequence: Chimeric Protein (FGF19(25-177)/FGF21 (177-209)
MW: 20KD
Tag: His tag
Source: E. Coli expressed
Purity: 95% in SDS-PAGE Gel
Formulation: PBS lyophilized
Storage: -20 oC ~ -70oC
Data Sheet: PDF

Anti Human FGF19 IgG
Code No.:00148-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Immunohen: Human FGF19, Rec
Antibody Type:Polyclonal IgG
Purification:Protein A affinity
Carry: free
Formulation: PBS lyophilized
Storage: -20 oC ~ -70oC
Data Sheet: PDF
Anti Human FGF19 IgG
Code No.:00148-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Immunohen: Human FGF19, Rec
Antibody Type:Polyclonal IgG
Purification:Protein A affinity
Carry: free
Formulation: PBS lyophilized
Storage: -20 oC ~ -70oC
Data Sheet: PDF

 

 
 

 

Name
Code No.
Size
Price ($)
Human FGF19 Ultrasensitive ELISA Kit
SK00148-09
96 T
490.00
Human FGF19 ELISA Kit
96 T
460.00
Human FGF19 ELISA Kit
192 T
720.00
Human FGF19 Recombinant
100 ug
360.00
Human FGF19-21 Chimera
100 ug
580.00
Anti FGF-19 (Human) IgG
100 ug
220.00