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Glia Maturation Factor Beta (GMF-B)

Alternative name:

  • GMF-B
This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability
signals. Two transcript variants encoding different isoforms have been found for this gene.
Serous ovarian carcinoma (SOC) is the most common subtype of epithelial ovarian cancer which remains the leading cause of death from gynaecologic malignancy. Further knowledge of the proteins involved in serous ovarian cancer may lead to new treatment targets, new markers for early detection or prognosis prediction. In this study, we applied proteomic techniques to analyse the protein expression profiles of SOC and normal ovarian epithelium tissues. Totally 54 aberrantly expressed proteins were identified using 2-DE combined with MALDI-TOF/TOF. Six of these proteins were validated by western blot. Corresponding gene expression analysis of these proteins was also performed using real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). Additionally, we analysed glia maturation factor beta (GMFB) protein expression by immunohistochemistry in 246 patients with various degrees of ovarian epithelial lesions. GMFB expression in SOC was found to be significantly enhanced than that in normal epithelium, benign serous adenoma and borderline serous adenoma tissues, and was positively correlated with FIGO stage (P=0.012). High GMFB expression was associated with poor disease-free survival (P=0.010) and overall survival (P=0.003), while multivariate analysis revealed GMFB to be an independent prognostic factor for disease-free survival (P=0.026) and overall survival (P=0.006) in patients with SOC. We therefore propose that proteins identified here may be involved in the development or progression of SOC, and GMFB can be considered as a prognostic predictor for SOC patients.
Li YL, et al. Eur J Cancer. 2010 Jul;46(11):2104-18. Epub 2010 May 22.
The purpose of this study was to screen for genes involved in ovarian carcinogenesis in an attempt to develop an effective molecular-targeted therapy for ovarian cancer. We constructed retroviral expression libraries for the human ovarian cancer cell lines SHIN-3 and TYK-CPr, and performed a focus formation assay with 3T3 cells. As a result, proteasome subunit beta-type 2 (PSMB2), ubiquitin-specific protease 14 (USP14), and keratin 8 (KRT8) were identified from SHIN-3, and polymerase II RNA subunit (POLR2E), chaperonin containing T-complex polypeptide 1 subunit 4 (CCT4), glia maturation factor beta (GMFB), and neuroblastoma ras viral oncogene homolog (NRAS) from TYK-CPr. NRAS gene analysis revealed a CAA --> AAA substitution at codon 61, resulting in a Glu --> Lys change at position 61. When the mutant NRAS was introduced into fibroblasts for its expression, many transformed foci were generated, confirming the transforming ability of the mutant NRAS.
Wada T, et al. Int J Oncol. 2009 Nov;35(5):973-6.
Anti Human GMF-Beta IgG
Code No.: A00659-01-100
Size: 100 ug
Price: $360.00 USD
Host: Rabbit
Antigen: human GMF-Beta Rec.
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: E, IHC
Working Dilution: 2 ug/ml)
Data Sheet: PDF
human GMF-B recombinant
Human GMF-B Recombinant
Code No.: 00659-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID:P60983
Gene ID: 2764
MW:16.6 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 80%
Data Sheet: PDF

 

Name
Code No.
Size
Price ($)
GMF-B (Human) recombinant
00659-01-10
10 ug
80.00
GMF-B (Human) recombinant
00659-01-50
50 ug
250.00
GMF-B (Human) recombinant
100 ug
360.00
GMF-B (Human) recombinant
00659-01-1000
1000 ug
3200.00
Rabbit Anti GMF-B (Human) IgG
100 ug
360.00