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Soluble Her2/ErbB2 (human) ELISA Kit
A Biomarker for Breast Cancer
Soluble Her1/ErbB1 (human) ELISA Kit
Soluble Her1/ErbB1 (human) ELISA Kit
  • Tyrosine kinase-type cell surface receptor HER2 (Her2)
  • Metastatic lymph node gene 19 protein
  • Receptor tyrosine-protein kinase erbB-2
  • Proto-oncogene c-ErbB-2
  • CD_antigen=CD340
  • Breast Cancer Biomarker
 
 

Challenges in the clinical utility of the serum test for HER2 ECD

Approximately 15-30% of breast cancers over-express the HER2/neu receptor. Historically, over-expression of HER2/neu has been identified using IHC or FISH, both of which are invasive approaches requiring tissue samples. Recent evidence has shown that some tumors identified as "negative" using these methods can respond to HER2/neu targeted therapy. Shedding of the extracellular domain (ECD) of the receptor into the circulation has led to the development of a serum test of HER2 ECD as an additional approach to probe HER2/neu overexpression. The serum test will be able to monitor the dynamic changes of HER2 status over the course of disease progression. Some studies further suggest that the serum HER2 ECD level and its change may serve as a biomarker to reflect patients' response to therapy. Yet more than 10years after the first serum HER2 ECD test was approved by the FDA, serum HER2 testing has yet to be widely used in clinical practice. In this article we will review the progress of the serum HER2ECD test and discuss some obstacles impeding its incorporation into broad clinical practice. We will also discuss recent improvements in the sensitivity and specificity of the assay that offer some hope for the future of serum HER2 test.
Lam L et al. Biochim Biophys Acta. 2012 Aug;1826(1):199-208. doi: 10.1016/j.bbcan.2012.03.012. Epub 2012 Apr 3.

HER2 shedding and serum HER2 extracellular domain: biology and clinical utility in breast cancer

The transmembrane protein HER2 is over-expressed in approximately 15% of invasive breast cancers as a result of HER2 gene amplification. HER2proteolytic cleavage (HER2 shedding) generates soluble truncated HER2 molecules that include only the extracellular domain and the concentration of which can be measured in the serum fraction of blood. HER2 shedding also generates a constitutively active truncated intracellular receptor of 95kDa (p95(HER2)). Another soluble truncated HER2 protein (Herstatin), which can also be found in serum, is the product of an alternatively splicedHER2 transcript. Recent preclinical findings may provide crucial insights into the biological and clinical relevance of increased sHER2 concentrations for the outcome of HER2-positive breast cancer and sensitivity to trastuzumab and lapatinib treatment. We present here the most recent findings about the role and biology of sHER2 based on data obtained using a standardized test, which has been cleared by FDA in 2000, for measuring sHER2. This test includes quality control assessments and has been already widely used to evaluate the clinical utility of sHER2 as a biomarker in breast cancer. We will describe in detail data concerning the assessment of sHER2 as a surrogate maker to optimize the evaluation of the HER2status of a primary tumor and as a prognosis and predictive marker of response to therapies, both in early and metastatic breast cancer.
Tsé C et al. Cancer Treat Rev. 2012 Apr;38(2):133-42. doi: 10.1016/j.ctrv.2011.03.008. Epub 2011 May 5.
 
 
human soluble Her2 elisa kit sk00226-06 from aviscera bioscience
Soluble Her2 (Human)ELISA Kit
Code No.: SK00226-06
Size: 96 T
Price: $360.00 USD
Standard range: 46.875-3000 pg/ml
Sensitivity: 10 pg/ml
Sample type: serum, plasma, cell cultures
Intra-CV: 4-6%
Inter-CV:8-12%
Protocol: PDF
human soluble egfr elisa kit sk00469-01
Soluble EGFR/Her1 (Human) ELISA Kit
Code No.: SK00469-01
Size: 96 T
Price: $360.00 USD
Standard range: 15.6-1000 pg/ml
Sensitivity: 10 pg/ml
Sample type: serum, plasma, cell cultures
Dilution factor: 100 for serum or plasma
Sample require: 100uL of diluted samples per well
Intra-CV: 4-6%
Inter-CV:8-10%
Protocol: PDF
  Soluble ErbB3/Her3 (Human) ELISA Kit
Code No.: SK00469-07
Size: 96 T
Price: $390.00 USD
Standard range: 23.44-1500 pg/ml
Sensitivity: 10 pg/ml
Sample type: serum, plasma, cell cultures
Dilution factor:
Sample require: 100uL of diluted samples per well
Intra-CV: 4-6%
Inter-CV:8-10%
Protocol: PDF

 

Anti Human Her2 IgG
Code No.: A00226-01-50
Size: 50 ug
Price: $220.00 USD
Host: Rabbit
Antigen: human Her2, ECD, Rec.
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: WB, IHC

 

Name
Code No.
Size
Price ($)
Soluble Her2/ErbB2 (Human) ELISA kit SK00226-06 96 T 360.00
soluble Her2/ErbB2 (Human) ELISA Kit SK00226-01 96 T  360.00
soluble Her1/ErbB1 (Human) ELISA Kit SK00469-01 96 T  360.00
soluble Her3/ErbB3 (Human) ELISA Kit SK00469-07 96 T  390.00
Her2/ErbB2 (Human) standard (human cell derived)
00226-06-10
10 ug
120.00
Rabbit Anti Her2 (H) IgG for wb, IFC
A00226-01-50
50 ug
220.00
Anti Her2/ErbB2 (Human) monoclonal antibody
A00226-09-100
100 ug
220.00
Anti Her2/ErbB2 (Human) monoclonal antibody
A00226-10-100
100 ug
220.00
Anti Her2/ErbB2 (Human) monoclonal antibody
A00226-10-100
100 ug
220.00