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Interleukin-1 beta ELISA, Recombinant Protein, Antibody
  • IL-1beta
IL-1beta is a member of the interleukin 1 cytokine family. Il-1beta is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form (117-269) by caspase 1 (CASP1/ICE). IL-1beta is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.
Central obesity is frequently associated with adipose tissue inflammation and hepatic insulin resistance. To identify potential individual mediators in this process, we used in vitro systems and assessed if insulin resistance in liver cells could be induced by secreted products from adipocytes preexposed to an inflammatory stimulus. Conditioned medium from 3T3-L1 adipocytes pretreated without (CM) or with TNFalpha (CM-TNFalpha) was used to treat Fao hepatoma cells. ELISAs were used to assess the concentration of several inflammatory mediators in CM-TNFalpha. CM-TNFalpha-treated Fao cells exhibited about 45% diminution in insulin-stimulated phosphorylation of insulin receptor, insulin receptor substrate proteins, protein kinase B, and glycogen synthase kinase-3 as compared with CM-treated cells, without changes in the total abundance of these protein. Insulin increased glycogenesis by 2-fold in CM-treated Fao cells but not in cells exposed to CM-TNFalpha. Expression of IL-1beta mRNA was elevated 3-fold in TNFalpha-treated adipocytes, and CM-TNFalpha had 10-fold higher concentrations of IL-1beta but not TNFalpha or IL-1alpha. IL-1beta directly induced insulin resistance in Fao, HepG2, and in primary rat hepatocytes. Moreover, when TNFalpha-induced secretion/production of IL-1beta from adipocytes was inhibited by the IL-1 converting enzyme (ICE-1) inhibitor II (Ac-YVAD-CMK), insulin resistance was prevented. Furthermore, liver-derived cells treated with IL-1 receptor antagonist were protected against insulin resistance induced by CM-TNFalpha. Finally, IL-1beta secretion from human omental fat explants correlated with body mass index (R(2) = 0.639, P < 0.01), and the resulting CM induced insulin resistance in HepG2 cells, inhibitable by IL-1 receptor antagonist. Our results suggest that adipocyte-derived IL-1beta may constitute a mediator in the perturbed cross talk between adipocytes and liver cells in response to adipose tissue inflammation. Nov O, et al.  Endocrinology. 2010 Sep;151(9):4247-56. Epub 2010 Jul 21.
Human IL_1beta ELISA
Code No.: SK00746-01
Size: 96 T
Price: $360.00 USD
Standard Range:3.9-500 pg/ml
Dynamic Range: 3.9-500 pg/ml
Sensitivity:3.9 pg/ml
Sample Type: serum, EDTA plasma
Sample requres: 220 ul, 100 ul per well
IntraCV: 6-8%
InterCV: 12-14%
Protocol: PDF
Mouse IL_1beta ELISA
Code No.: SK00746-03
Size: 96 T
Price: $390.00 USD
Standard Range:7.8-500 pg/ml
Dynamic Range: 7.8-500 pg/ml
Sensitivity:3.9 pg/ml
Sample Type: serum, EDTA plasma
Sample requres: 220 ul, 100 ul per well
IntraCV: 6-8%
InterCV: 12-14%
Protocol: PDF
 
 
human IL-1beta recombinant Human IL-1Beta Recombinant
Code No.: 00746-01-100
Size: 100 ug
Price: $295.00 USD
Protein ID:P01584
Gene ID: 3553
MW: 16.8 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
   

 

 
Anti Human IL-1beta IgG
Code No.: A00746-01100
Size: 100 ug
Price: $260.00 USD
Host: Rabbit
Antigen:Human IL-1beta recombinant
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: E, IHC,
Working Dilution: IHC 1: 500 (2 ug/ml)
Data Sheet: PDF

 

Name
Code No.
Size
Price ($)
Human IL-1beta  ELISA KIt SK00746-01 96 T 360.00
Human IL-1beta  ELISA KIt SK00746-01B 480 T 1500.00
Mouse IL-1beta  ELISA KIt SK00746-03 96 T 390.00
IL-1Beta (Human), Rec.
00746-01-50
50 ug
180.00
IL-1beta (Human), Rec.
100 ug
295.00
IL-1beta (Human), Rec.
00746-01-1000
1 mg
inquire
IL_1beta (H), Rec. Biotinylated
00746-01-50B
50 ug
360.00
IL-1beta(H), Rec. FITC Conjugated
00746-01-50F
50 ug
360.00
IL-1beta (H) , Rec. Cy3 Conjugated
00746-01-50C3
50 ug
460.00
Rabbit Anti IL-1beta (H) IgG
100 ug
260.00
Rabbit Anti  IL-1beta (H), Biotinylated
A00746-01-50B
50 ug
390.00
Rabbit Anti IL-1beta (H), Cy3 Conjugated
A00746-01-50C3
50 ug
460.00
IL-1Beta (Human), Rec. (CHO derived)
00746-02-10
10 ug
190.00