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Soluble IL-1 Receptor 3 (sIL-1R AcP) ELISA Kit
A Novel Biomarker for Heart Failure

Alternative name:

  • Soluble IL-1 R3
  • Soluble IL-1R AcP
  • IL-1 receptor accessory protein
Interleukin (IL)-33 is an important member of the IL-1 family that has pleiotropic activities in innate and adaptive immune responses in host defense and disease. It signals through its ligand-binding primary receptor ST2 and IL-1 receptor accessory protein (IL-1RAcP), both of which are members of the IL-1 receptor family. To clarify the interaction of IL-33 with its receptors, we determined the crystal structure of IL-33 in complex with the ectodomain of ST2 at a resolution of 3.27 Å. Coupled with structure-based mutagenesis and binding assay, the structural results define the molecular mechanism by which ST2 specifically recognizes IL-33. Structural comparison with other ligand-receptor complexes in the IL-1 family indicates that surface-charge complementarity is critical in determining ligand-binding specificity of IL-1 primary receptors. Combined crystallography and small-angle X-ray-scattering studies reveal that ST2 possesses hinge flexibility between the D3 domain and D1D2 module, whereas IL-1RAcP exhibits a rigid conformation in the unbound state in solution. The molecular flexibility of ST2 provides structural insights into domain-level conformational change of IL-1 primary receptors upon ligand binding, and the rigidity of IL-1RAcP explains its inability to bind ligands directly. The solution architecture of IL-33-ST2-IL-1RAcP complex from small-angle X-ray-scattering analysis resembles IL-1β-IL-1RII-IL-1RAcP and IL-1β-IL-1RI-IL-1RAcP crystal structures. The collective results confer IL-33 structure-function relationships, supporting and extending a general model for ligand-receptor assembly and activation in the IL-1 family.
Liu X. et al., Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):14918-23
CONTEXT: Adipokines actuate chronic, low-grade inflammation through a complex network of immune markers, but the current understanding of these networks is incomplete. The soluble isoform of the IL-1 receptor accessory protein (sIL1RAP) occupies an important position in the inflammatory pathways involved in obesity. The pathogenetic and clinical influences of sIL1RAP are unknown.
OBJECTIVE:The objective of the study was to elucidate whether plasma levels of sIL1RAP are reduced in obesity, using affluent clinical, biochemical, and genetic data from two diverse cohorts.
DESIGN, SETTING, AND PARTICIPANTS:The study was conducted in two cohorts: the San Antonio Family Heart Study (n = 1397 individuals from 42 families) and South Asians living in Mauritius, n = 230).
MAIN OUTCOME MEASURES:Plasma sIL1RAP levels were measured using an ELISA. The genetic basis of sIL1RAP levels were investigated using both a large-scale gene expression profiling study and a genome-wide association study.
RESULTS:A significant decrease in plasma sIL1RAP levels were observed in obese subjects, even after adjustment for age and sex. The sIL1RAP levels demonstrated a strong inverse association with obesity measures in both populations. All associations were more significant in females. Plasma sIL1RAP levels were significantly heritable, correlated with IL1RAP transcript levels (NM_134470), showed evidence for shared genetic influences with obesity measures and were significantly associated with the rs2885373 single-nucleotide polymorphism (P = 6.7 × 10(-23)) within the IL1RAP gene.
CONCLUSIONS:Plasma sIL1RAP levels are reduced in obesity and can potentially act as biomarkers of obesity. Mechanistic studies are required to understand the exact contribution of sIL1RAP to the pathogenesis of obesity.

Bozaoglu K, et al. J Clin Endocrinol Metab. 2014 Sep;99(9):3435-43
human soluble IL-1R3 elisa kit from aviscera bioscience
Human Soluble IL-1R AcP/IL-1R3 ELISA Kit
Code No.: SK00118-20
Size: 96T
Price: $360.00 USD
Standard Range: 40 - 2560 pg/mL
Sensitivity:10 pg/mL
Sample Type: serum, EDTA Plasma
Sample volume: 100 uL
IntraCV: 4-6%
InterCV: 8-12%
Protocol: PDF
human soluble ST2 IL1RL1 elisa  kit from aviscera bioscience
Human Soluble ST2/IL-1R4ELISA Kit
Code No.: SK00120-06
Size: 96T
Price: $360.00 USD
Standard Range:31-800 pg/mL
Sensitivity:15 pg/mL
Sample Type: serum, EDTA Plasma
Sample volume: 100 uL
Dilution factor: 8-16 (Optimal dilutions should be determined by each laboratory for each application)
IntraCV: 4-6%
InterCV: 8-12%
Protocol: PDF
Mouse Soluble ST2/IL-1R4ELISA Kit
Code No.: SK00120-03
Size: 96T
Price: $360.00 USD
Standard Range: 0.312-20 ng/mL
Sensitivity:0.15 ng/mL
Sample Type: serum, EDTA Plasma
Sample volume: 100 uL
Dilution factor: 5
IntraCV: 4-6%
InterCV: 8-12%
Protocol: PDF
Soluble IL-1R2 Ultra-sensitive (Human) ELISA Kit
Code No.: SK00119-06
Size: 96T
Price: $360.00 USD
Standard Range:9.375-600 pg/mL
Sensitivity:5 pg/mL
Sample Type: serum, EDTA Plasma
Sample volume: 100 uL
Dilution factor: Optimal dilutions should be determined by each laboratory for each application
IntraCV: 4-6%
InterCV: 8-12%
Protocol: PDF
Human Soluble IL-1R2 ELISA Kit
Code No.: SK00119-02
Size: 96T
Price: $360.00 USD
Standard Range:31-4000 pg/mL
Sensitivity:15 pg/mL
Sample Type: serum, EDTA Plasma
Sample volume: 100 uL
Dilution factor: Optimal dilutions should be determined by each laboratory for each application
IntraCV: 4-6%
InterCV: 8-12%
Protocol: PDF
Human Soluble IL-1R1 ELISA Kit
Code No.: SK00118-06
Size: 96T
Price: $360.00 USD
Standard Range:62.5-4000 pg/mL
Sensitivity:30 pg/mL
Sample Type: serum, EDTA Plasma
Sample volume: 100 uL
Dilution factor: Optimal dilutions should be determined by each laboratory for each application
IntraCV: 4-6%
InterCV: 8-12%
Protocol: PDF
Rat Soluble IL-1R1 ELISA Kit
Code No.: SK00118-07
Size: 96T
Price: $360.00 USD
Standard Range:62.5-4000 pg/mL
Sensitivity:30 pg/mL
Sample Type: serum, EDTA Plasma
Sample volume: 100 uL
Dilution factor: Optimal dilutions should be determined by each laboratory for each application
IntraCV: 4-6%
InterCV: 8-12%
Protocol: PDF
 

 

Name
Code No.
Size
Price ($)
Soluble IL-1R AcP/IL-1R3 (Human) ELISA Kit
96 T
360.00