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Interleukin 4(IL-4) ELISA
 

IL-4/STAT6 immune axis regulates peripheral nutrient metabolism and insulin sensitivity

Immune cells take residence in metabolic tissues, providing a framework for direct regulation of nutrient metabolism. Despite conservation of this anatomic relationship through evolution, the signals and mechanisms by which the immune system regulates nutrient homeostasis and insulin action remain poorly understood. Here, we demonstrate that the IL-4/STAT6 immune axis, a key pathway in helminth immunity and allergies, controls peripheral nutrient metabolism and insulin sensitivity. Disruption of signal transducer and activator of transcription 6 (STAT6) decreases insulin action and enhances a peroxisome proliferator-activated receptor α (PPARα) driven program of oxidative metabolism. Conversely, activation of STAT6 by IL-4 improves insulin action by inhibiting the PPARα-regulated program of nutrient catabolism and attenuating adipose tissue inflammation. These findings have thus identified an unexpected molecular link between the immune system and macronutrient metabolism, suggesting perhaps the coevolution of these pathways occurred to ensure access to glucose during times of helminth infection.
This study examines the differences in circulating levels of cytokines among Japanese in Japan (JJ), Japanese Americans (JA), and whites and their associations with obesity and marine n-3 fatty acids (FA) in a cross-sectional population-based study of 297 men aged 40-49 (100 JJ, 99 whites, and 98 JA). Experimental studies show that cytokines are associated with obesity positively and marine n-3 FA inversely. Serum interleukin-1alpha (IL-1alpha), IL-1 receptor agonist (IL-1ra), IL-4, IL-8, IL-10, inducible protein-10 (IP-10), tumor necrosis factor-alpha (TNF-alpha), monocyte chemoattractant protein-1 (MCP-1), and marine n-3 FA were determined. Body mass index (BMI), waist circumference, and computed tomography-measured visceral and subcutaneous adipose tissues were determined. The JJ had significantly lower levels of IL-1alpha, IL-4, IL-8, MCP-1, and TNF-alpha than whites and JA. Whites and JA had similar levels of IL-1alpha, IL-4, and IL-8 whereas whites had significantly higher levels of MCP-1 and TNF-alpha than JA. The JJ were least obese (BMI (kg/m(2)), mean +/- standard deviation) 23.6 +/- 2.8, 27.9 +/- 4.6, and 27.9 +/- 4.5 for JJ, whites, and JA, respectively. The JJ had marine n-3 FA about 100% higher than whites and JA (serum marine n-3 FA (%), median (interquartile range) 8.79 (7.41, 11.16), 3.47 (2.63, 4.83), and 4.44 (3.33, 6.01) for JJ, whites, and JA, respectively). Generally cytokines had weak and nonsignificant associations with indices of obesity and nonsignificant associations with marine n-3 FA. BMI had significant inverse associations with IL-1alpha, IL-4, and IL-8 in JA (P < 0.05). Marine n-3 FA had marginally significant inverse associations with IL-8 in JJ (P = 0.055) and TNF-alpha in whites (P = 0.076). The JJ had lower levels of many cytokines than whites and JA. Generally cytokines had weak and nonsignificant associations with indices of obesity and marine n-3 FA. Further investigation is needed to determine why JJ had lower circulating levels of cytokines.
Sekikawa A, , et al. .J Interferon Cytokine Res. 2010 Jul;30(7):541-8.
  Human IL-4 ELISA
Code No.: SK00748-01
Size: 96 T
Price: $360.00 USD
Standard Range:3.12-200 pg/ml
Dynamic Range: 3.12-200 pg/ml
Sensitivity:  0.7 pg/ml
Sample Type: serum, EDTA plasma
Sample requres: 100 uL per well
IntraCV: 6-8%
InterCV: 10-12%
Protocol: PDF
anti human IL-4 antibody form IHC Anti Human IL-4 IgG
Code No.: A00748-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Antigen: human IL-4 Rec.
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: E, IHC
Working Dilution: 2 ug/ml)
Data Sheet: PDF
human IL-4 recombinant
Human IL-41 Recombinant/span>
Code No.: 00748-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID:P05112
Gene ID: 3565
MW:16.7 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 90%
Data Sheet: PDF

 

Name
Code No.
Size
Price ($)
IL-4 (Human) Ultrasensitive ELISA Kit SK00748-01 96 T 360.00
IL-4 (Human) recombinant
00748-01-50
50 ug
250.00
IL-4 (Human) recombinant
100 ug
360.00
IL-4 (Human) recombinant
00748-01-1000
1000 ug
3200.00
Anti IL-4 (Human) IgG
100 ug
220.00