AVISCERA BIOSCIENCE  
line decor
  HOME  ::  
line decor
   
 
o
Macrophage migration Inhibitory Factor (MIF)  (Human) ELISA Kit, Recombinant
 

A New Biomarker for Acute Myocardial Infarction (AMI)

Macrophage migration inhibitory factor for the early prediction of infarct size.

Chan W, et al.J Am Heart Assoc. 2013 Oct 4;2(5):e000226.

Macrophage migration inhibitory factor in myocardial ischaemia/reperfusion injury

Rassaf T et al. Cardiovasc Res. 2014 May 1;102(2):321-8.
human MIF elisa kit from aviscera bioscience Human Macrophage migration inhibitory Factor (MIF) ELISA
Code No.: SK00262-01
Size: 96 T
Price: $360.00 USD
Standard Range:31.25-2000 pg/ml
Sensitivity:15 pg/ml
Sample Type: serum, plasma
Dilution factor: 50-100
Sample requres: 100uL per well ,
IntraCV:4-6%
InterCV: 10-12%
Protocol: PDF
human MIF recombinant from aviscera bioscience Human MIF Rec
Code No.: 00262-04-100
Size: 96 T
Price: $360.00 USD
Protein ID :NP_002406
Gene ID : NM_002415
Tag; His Tag on N-terminus
MW:16.3 KD
Purity: >95% in SDS-PAGE gel
Data Sheet: PDF
Anti Human MIF IgG
Code No.: A00262-01-100
Size: 100 ug
Price: $269.00 USD
Antibody Type: Polyclonal
Host: Rabbit
Immunogen: Human MIF Rec
Target Protein ID :NP_002406
Target Gene ID : NM_002415
Data Sheet: PDF

 

Product
Code
Size
Price ($)
Macrophage Migration Inhibitory Factor (MIF) (Human) ELISA Kit
96 T
360.00
Macrophage Migration Inhibitory Factor (MIF) (Mouse) ELISA Kit
SK00262-03
96 T
390.00
Macrophage Migration Inhibitory Factor (MIF) (Human) Rec.
00262-04-10
10 ug
90.00
Macrophage Migration Inhibitory Factor (MIF) (Human) Rec.
00262-04-50
50 ug
250.00
Macrophage Migration Inhibitory Factor (MIF) (Human) Rec.
100 ug
360.00
Macrophage Migration Inhibitory Factor (MIF) (Human) Rec.
00262-04-1000
1000 ug
inquire
Macrophage Migration Inhibitory Factor (MIF) (Human) Rec. Biotinylated
00262-04-50B
50 ug
460.00
Macrophage Migration Inhibitory Factor (MIF) (Human) Rec. Cy3 conjugated
00262-04-50C3
50 ug
490.00
       
Macrophage Migration Inhibitory Factor (MIF) (Human) Rec.
00262-01-20
20 ug
290.00
Macrophage Migration Inhibitory Factor (MIF) (Mouse) Rec.
00262-01-20
20 ug
290.00
Anti Macrophage Migration Inhibitory Factor (MIF) (Human) IgG
100 ug
269.00
Anti Macrophage Migration Inhibitory Factor (MIF) (Human) IgG Biotinylated
50 ug
350.00
       
Human MIF recombinant protein was from aviscera bioscience
Referenes:
Macrophage migration inhibitory factor deficiency ameliorates high-fat diet induced insulin resistance in mice with reduced adipose inflammation and hepatic steatosis
Macrophage infiltration is a critical determinant of high-fat diet induced adipose tissue inflammation and insulin resistance. The precise mechanisms underpinning the initiation of macrophage recruitment and activation are unclear. Macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine, displays chemokine-like properties. Circulating MIF levels are elevated during obesity however its role in high-fat diet induced adipose inflammation and insulin resistance remains elusive. Wildtype and MIF-/- C57Bl\6J mice were fed chow or high-fat diet. Body weight and food intake was assessed. Glucose homeostasis was monitored by glucose and insulin tolerance tests. Adipose tissue macrophage recruitment and adipose tissue insulin sensitivity was evaluated. Cytokine secretion from stromal vascular fraction, adipose explants and bone marrow macrophages was measured. Inflammatory signature and insulin sensitivity of 3T3-L1-adipocytes co-cultured with wildtype and MIF-/- macrophage was quantified. Hepatic triacylglyceride levels were assessed. MIF-/- exhibited reduced weight gain. Age and weight-matched obese MIF-/- mice exhibited improved glucose homeostasis coincident with reduced adipose tissue M1 macrophage infiltration. Obese MIF-/- stromal vascular fraction secreted less TNFa and greater IL-10 compared to wildtype. Activation of JNK was impaired in obese MIF-/-adipose, concomitant with pAKT expression. 3T3-L1-adipocytes cultured with MIF-/- macrophages had reduced pro-inflammatory cytokine secretion and improved insulin sensitivity, effects which were also attained with MIF inhibitor ISO-1. MIF-/- liver exhibited reduced hepatic triacyglyceride accumulation, enhanced pAKT expression and reduced NF?B activation. MIF deficiency partially protects from high-fat diet induced insulin resistance by attenuating macrophage infiltration, ameliorating adipose inflammation, which improved adipocyte insulin resistance ex vivo. MIF represents a potential therapeutic target for treatment of high-fat diet induced insulin resistance.
PLoS One. 2014 Nov 20;9(11):e113369. doi: 10.1371/journal.pone.0113369. eCollection 2014.
Suppressed expression of macrophage migration inhibitory factor in the oviducts of lean and obese cows
Oviducts synthesise macrophage migration inhibitory factor (MIF) to promote sperm capacitation and embryogenesis. This study aimed to test a hypothesis that the oviducts of obese cows may express MIF at a lower level than those of normal and lean cows. Ampullar and isthmic oviduct sections were collected from lean (n=5; body condition score (BCS) on a 5-point scale, 2.5), normal (n=6; BCS, 3.0) and obese (n=5; BCS, 4.0) Japanese Black cows. MIF mRNA and protein were extracted from ampullae and isthmuses and their levels measured by real-time polymerase chain reaction or western blot. Immunohistochemistry was performed on frozen sections of ampullae and isthmuses by using antibodies to MIF. MIF mRNA and protein expression were lower in the obese and lean groups than in the normal group (P<0.05). Immunohistochemistry revealed that the primary site of MIF expression in the ampulla and isthmus is the tunica mucosa. In conclusion, obese cows have suppressed MIF expression in the ampullae and isthmuses of their oviducts, as hypothesised, but, unexpectedly, MIF expression was also lower in lean cows.
Reprod Fertil Dev. 2014 Nov 25. doi: 10.1071/RD14164.