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MIP-1 alpha ELISA

Alternative name:

  • CCL3
  • Small-inducible cytokine A3
  • Macrophage inflammatory protein 1-alpha

Asthma studies suggest that alteration in the inflammation pattern may be associated with the severity of asthma. The aim of this study was to compare in vitro the expression of chemokines, chemokine receptors and cytokine production from CD4+ T human lymphocytes of asthmatic, both obese and non-obese patients with different severity levels of asthma. Lymphocytes were labeled with monoclonal anti-human CXCR3/IP-10, MIP-1a/CCR5 antibodies and were analyzed by flow cytometry. Cell culture supernatants were used to measure production of interleukin IL-6 and resistin by ELISA. CXCR3/IP-10 expression increased in non-obese patients with mild persistent asthma (2.2%, p<0.05), moderate persistent asthma (3%, p<0.003) and severe persistent asthma (4%, p<0.004); this effect was stronger in obese patients with severe persistent asthma (35%, p<0.004). MIP-1 α / CCR5 increased in non-obese patients with intermittent asthma (0.65%, p<0.05) and severe asthma (1.4%, p<0.03); in obese patients, this expression was greater in intermittent asthma (8%, p<0.05) and severe persistent asthma (12%, p<0.04). Resistin production strongly increased in obese patients with intermittent (976 ng/ml) and severe persistent asthma (795 ng/ml). IL-6 increased in both lean and obese persons; however, the highest value was registered in the group of severe persistent obese asthmatics (992 pg/ml). Obesity per se increased the inflammatory profile of chemokines / cytokines secreted by cells of the blood, increasing the inflammatory status in asthmatic patients. Resistin showed characteristics of a pro-inflammatory cytokine mainly in severely obese asthmatics.

Rojas-Dotor S,, et al. Biol Res. 2013;46(1):13-20. doi: 10.4067/S0716-97602013000100002.

Human MIP-1alpha/CCL3 ELISA Kit
Code No.: SK00573-01
Size: 96T
Price: $360.00 USD
Standard Range:15.6-1000 pg/mL
Sensitivity:8 pg/mL
Sample Type: serum, EDTA plasma
Dilution factor: 2 for serum and plasma
Sample Volume: 100 uL
IntraCV: 4-6%
InterCV: 8-12%
Protocol: PDF

 

Name
Code No.
Size
Price ($)
Human MIP-1alpha/CCL3 ELISA kt
96 T
360.00