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Soluble Mammalian Sterile 20–like kinase-1 (MST1) (Human) ELISA Kit
A New Target for Diabetes Research and Biomarker for Cancer
Apoptotic cell death is a hallmark of the loss of insulin-producing beta cells in all forms of diabetes mellitus. Current treatments fail to halt the decline in functional beta cell mass, and strategies to prevent beta cell apoptosis and dysfunction are urgently needed. Here, we identified mammalian sterile 20–like kinase-1 (MST1) as a critical regulator of apoptotic beta cell death and function. Under diabetogenic conditions, MST1 was strongly activated in beta cells in human and mouse islets and specifically induced the mitochondrial-dependent pathway of apoptosis through upregulation of the BCL-2 homology-3 (BH3)-only protein BIM. MST1 directly phosphorylated the beta cell transcription factor PDX1 at T11, resulting in the latter's ubiquitination and degradation and thus in impaired insulin secretion. MST1 deficiency completely restored normoglycemia, beta cell function and survival in vitro and in vivo. We show MST1 as a proapoptotic kinase and key mediator of apoptotic signaling and beta cell dysfunction and suggest that it may serve as target for the development of new therapies for diabetes.
Amin Ardestani et al.Nature Medicine online. 2014, March 16
BACKGROUND: Skeletal muscle undergoes rapid atrophy upon denervation and the underlying mechanisms are complicated. FOXO3a has been implicated as a major mediator of muscle atrophy, but how its subcellular location and activity is controlled during the pathogenesis of muscle atrophy remains largely unknown. MST1 (Mammalian Sterile 20-like kinase 1) is identified as a central component of the Hippo signaling pathway. MST1 has been shown to mediate phosphorylation of FOXO3a at Ser207. Whether this MST1-FOXO signaling cascade exerts any functional consequence on cellular homeostasis remains to be investigated.
RESULT: We identified that MST1 kinase was expressed widely in skeletal muscles and was dramatically up-regulated in fast- but not slow-dominant skeletal muscles immediately following denervation. The results of our histological and biochemical studies demonstrated that deletion of MST1 significantly attenuated denervation-induced skeletal muscle wasting and decreased expression of Atrogin-1 and LC3 genes in fast-dominant skeletal muscles from three- to five-month-old adult mice. Further studies indicated that MST1, but not MST2, remarkably increased FOXO3a phosphorylation level at Ser207 and promoted its nuclear translocation in atrophic fast-dominant muscles.
CONCLUSIONS: We have established that MST1 kinase plays an important role in regulating denervation-induced skeletal muscle atrophy. During the early stage of muscle atrophy, the up-regulated MST1 kinase promoted progression of neurogenic atrophy in fast-dominant skeletal muscles through activation of FOXO3a transcription factors.
Wei B, et al. BMC Biol. 2013 Feb 1;11:12
RESULTS: Compared with the evaluation of common tumour features, the evaluation of individual tumour features gave a more comprehensive and detailed overview of the regulatory processes in endometrial tumours. Systemic analysis of the individual proteome profiles showed that endometrial tumours employed different proteins to regulate similar functions. Comparison of our data with publicly available data sets of molecular profiling of human endometrial tumours confirmed that individual tumour features are not simply irrelevant individual variations, but are indeed important in endometrial tumorigenesis. Validation through tissue microarray investigation of MST1 and PKN1 proteins confirmed the usefulness of this approach, and suggested that MST1 and PKN1 may be considered as predictive biomarkers of endometrial cancer.
Attarha S et al. Br J Cancer. 2013 Aug 6;109(3):704-13
MST1 (Human) ELISA Kit enables to detect human samples from aviscera bioscience Human Soluble MST1 ELISA Kit
Code No.: SK00501-01
Size: 96 T
Price: $460.00
Standard range:78-5000 pg/ml
Sensitivity: 39 pg/ml
Sample Type:serum, plasma
Sample require: 100 ul per well
Intra-CV:4-6%
Inter-CV: 8-12%
Data Sheet: PDF

 

Name
Code
Size
Price ($)

Soluble mammalian sterile 20–like kinase-1 (MST1) (Human) ELISA Kit

96 T

420.00

Soluble mammalian sterile 20–like kinase-1 (MST1) (Human) ELISA Kit

192 T

810.00

Soluble mammalian sterile 20–like kinase-1 (MST1) (Human) ELISA Kit

480 T

1995.00

Soluble mammalian sterile 20–like kinase-1 (MST1) (Human) ELISA Kit

960 T

3570.00

MST1 (Human) Standard.
00501-01-10
10 ug
190.00
Anti MST1 (Human) IgG
A00501-01-50
50 ug
360.00
Anti MST1 (Human) Monoclonal Antibody
A00501-02-50
50 ug
320.00