aviscera bioscience  
AVISCERA BIOSCIENCE  
line decor
  HOME  ::  
line decor
   
 

Neutrophil Gelactinase-Associated Lipocalin (NGAL)/Lipocalin-2 ELISA

A Potential Biomarker for Predicting Acute Kidney Injury, Nephritis , coronary heart disease & diabetes

Alternative name:

  • NGAL
  • lipocalin-2
  • LCN-2

Adipocytokines and insulin resistance: the possible role of lipocalin-2, retinol binding protein-4, and adiponectin

TLCN2 (or neutrophil gelatinase-associated lipocalin) is a recently identified adipokine that belongs to the superfamily of lipocalins (such as RBP4), which seems to affect glucose metabolism and insulin sensitivity . LCN2 protein has been implicated in diverse actions, such as apoptosis and innate immunity, and is expressed in several tissues, including neutrophils, liver, kidney, adipocytes, and macrophages . LCN2 is highly expressed in adipose tissue in vivo and in vitro, and its secretion is regulated by the activation of inflammation and infection . LCN2 mRNA is also elevated in the liver of ob/ob mice, and circulating LCN2 levels are increased in obese animals and human subjects with type 2 diabetes (5,8). In fact, expression of LCN2 is elevated by agents that promote insulin resistance and is reduced by thiazolidinediones . In one study, serum concentrations of LCN2 were positively associated with waist circumference, percent body fat, systolic blood pressure, fasting glucose and insulin concentrations, fasting triglycerides, and markers for chronic inflammation. All these results persisted significantly after adjustment for sex and age . In another study, serum LCN2 levels were significantly elevated in patients with coronary heart disease and were independently associated with atherosclerosis. 
Esteve E, Ricart W, Fernández-Real JM. Diabetes Care. 2009 Nov;32 Suppl 2:S362-7.

Lipocalin 2 is a selective modulator of peroxisome proliferator-activated receptor-γ activation and function in lipid homeostasis and energy expenditure

We have previously identified lipocalin 2 (Lcn2) as a cytokine playing a critical role in the regulation of body fat mass, lipid metabolism, and insulin resistance. Lcn2 deficiency reduces PPARγ gene expression in adipocytes. In this study, we investigated the role of Lcn2 in PPARγ activation and function via assessing the insulin sensitization and fatty acid (FA) homeostasis of PPARγ agonist in high-fat diet (HFD)-induced obesity in Lcn2−/− mice. We found that rosiglitazone (Rosi) significantly improved insulin sensitivity in Lcn2−/− mice as effectively as in wild-type (WT) mice; unfed-state levels of blood glucose, free FAs, and triglycerides (TGs) were significantly reduced after a 25-d treatment of Rosi in Lcn2−/− mice. However, Rosi action on fat deposition and FA homeostasis was altered; Rosi-induced body weight and subcutaneous fat gain and liver lipid accumulation were markedly lessened in Lcn2−/− mice. The results of in vivo metabolic labeling showed that Rosi markedly reduced de novo lipogenesis in adipose tissue of Lcn2−/− mice. In brown adipose tissue (BAT), the expression of the genes functioning in TG hydrolysis and mitochondrial oxidation was up-regulated more in Lcn2−/− than in WT mice. Most strikingly, Rosi stimulated significantly higher levels of uncoupling protein-1 expression in BAT, and completely rescued cold intolerance in Lcn2−/− mice. We demonstrate that Lcn2 is a critical selective modulator of PPARγ activation and function in lipid homeostasis and energy expenditure.
Daozhong Jin et al. February 2011,The FASEB Journal vol. 25 no. 2 754-764

Lipocalin-2 deficiency impairs thermogenesis and potentiates diet-induced insulin resistance in mice

  OBJECTIVE: Lipocalin (LCN) 2 belongs to the lipocalin subfamily of low-molecular mass-secreted proteins that bind small hydrophobic molecules. LCN2 has been recently characterized as an adipose-derived cytokine, and its expression is upregulated in adipose tissue in genetically obese rodents. The objective of this study was to investigate the role of LCN2 in diet-induced insulin resistance and metabolic homeostasis in vivo. RESEARCH DESIGN AND METHODS: Systemic insulin sensitivity, adaptive thermogenesis, and serum metabolic and lipid profile were assessed in LCN2-deficient mice fed a high-fat diet (HFD) or regular chow diet. RESULTS: The molecular disruption of LCN2 in mice resulted in significantly potentiated diet-induced obesity, dyslipidemia, fatty liver disease, and insulin resistance. LCN2(-/-) mice exhibit impaired adaptive thermogenesis and cold intolerance. Gene expression patterns in white and brown adipose tissue, liver, and muscle indicate that LCN2(-/-) mice have increased hepatic gluconeogenesis, decreased mitochondrial oxidative capacity, impaired lipid metabolism, and increased inflammatory state under the HFD condition. CONCLUSIONS: LCN2 has a novel role in adaptive thermoregulation and diet-induced insulin resistance.
Guo H et al. Diabetes. 2010 Jun;59(6):1376-85. Epub 2010 Mar 23.

 

human ngal elisa kit sk00233-01 from aviscera bioscience enables to measure urine samples

 
human ngal elisa kit from aviscera bioscience
Human Neutrophil Gelactinase Associated Lipocalin (NGAL) ELISA
Code No.: SK00233-01
Size: 96 T
Price: $360.00
Standard Range:19.5-1250 pg/ml
Sensitivity: 10 pg/ml
Sample Type: serum, plasma, urine
Sample Volume: 100 uL
Dilution Factor: 50-100
Intra CV: 4-6%
Inter CV: 8-10%
Protocol: PDF
rat mouse ngal elisa kit
Mouse/Rat Neutrophil Gelactinase Associated Lipocalin (NGAL) ELISA
Code No.: SK00233-06
Size: 96 T
Price: $360.00
Standard Range:1.6-1000 ng/ml
Sensitivity: 8 ng/ml
Sample Type: serum, plasma
Sample Volume: 50 uL
Dilution Factor: 4 for rat serum, 2 for mouse serum
Intra CV: 4-6%
Inter CV: 8-10%
Protocol: PDF
  Rat Neutrophil Gelactinase Associated Lipocalin (NGAL) ELISA
Code No.: SK00233-09
Size: 96 T
Price: $360.00
Standard Range:0.78-50 ng/ml
Sensitivity: 30 pg/ml
Sample Type: serum, plasma
Sample Volume: 100 uL
Dilution Factor:
Intra CV: 4-6%
Inter CV: 8-10%
Protocol: PDF
human lipocalin-1 elisa kit sk00487-01 from aviscera bioscience
Human Lipocalin-1ELISA
Code No.: SK00487-01
Size: 96 T
Price: $360.00
Standard Range:31-2000 pg/ml
Sensitivity: 10 pg/ml
Sample Type: serum, plasma
Sample Volume: 100 uL
Intra CV: 4-6%
Inter CV: 8-10%
Protocol: PDF
Anti Human NGAL   IgG
Code No.: A00233-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Antigen: human NGAL Rec.
Ab Type: Polyclonal
Purification: Protein A
Applications: E, IHC (1-2 ug/ml)
Data Sheet: PDF
  Anti Human NGAL   Monoclonal Antibody
Code No.: A00233-05-100
Size: 100 ug
Price: $260.00 USD
Host: Mouse
Antigen: human NGAL Rec.
Ab Type: Monoclonal
Purification: Protein G
Applications: ELISA
Data Sheet: PDF
  Anti Human NGAL   Monoclonal Antibody
Code No.: A00233-06-100
Size: 100 ug
Price: $260.00 USD
Host: Mouse
Antigen: human NGAL Rec.
Ab Type: Monoclonal
Purification: Protein G
Applications: ELISA
Data Sheet: PDF
  Anti Human NGAL   Monoclonal Antibody
Code No.: A00233-07-100
Size: 100 ug
Price: $260.00 USD
Host: Mouse
Antigen: human NGAL Rec.
Ab Type: Monoclonal
Purification: Protein G
Applications: ELISA
Data Sheet: PDF
  Anti Human NGAL   Monoclonal Antibody
Code No.: A00233-08-100
Size: 100 ug
Price: $260.00 USD
Host: Mouse
Antigen: human NGAL Rec.
Ab Type: Monoclonal
Purification: Protein G
Applications: ELISA
Data Sheet: PDF
   

Human NGAL Recombinant
Code No.: 00233-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID:P80188
Gene ID: 3934
MW: 19.6 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
Rat NGAL Recombinant
Code No.: 00233-02-100
Size: 100 ug
Price: $360.00 USD
Protein ID:P30152
Gene ID: 170496
MW: 22 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
anti rat ngal antibody Anti Rat NGAL   IgG
Code No.: A00233-02-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Antigen: Rat NGAL Rec.
Ab Type: Polyclonal
Purification: Protein A
Applications: E, IHC (1-2 ug/ml)
Data Sheet: PDF

 

Name Code Size Price ($)
Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) ELISA Kit SK00233-01 96 T 360.00
Rat/Mouse Neutrophil Gelactinase Associated Lipocalin (NGAL) ELISA Kit SK00233-06 96 T 360.00
Neutrophil Gelactinase Associated Lipocalin (NGAL) (Rat) ELISA Kit SK00233-09 96 T 360.00
Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) ELISA Set
SK00233-07
15 X 96T
649.00
Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human) ELISA Set
SK00233-17
5 x 96T
350.00
Lipocalin-1 (Human) ELISA Kit
96 T
360.00
Neutrophil Gelactinase Associated Lipocalin (NGAL)  (Human) Rec.
100 ug
360.00
Neutrophil Gelactinase Associated Lipocalin (NGAL)  (Human), Biotinylated
00233-01-50B
50 ug
490.00
Neutrophil Gelactinase Associated Lipocalin (NGAL)  (Rat) Rec 00233-02-100 100 ug 360.00
Anti Neutrophil Gelactinase Associated Lipocalin (NGAL)  (Human) IgG
100 ug
220.00
Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Human)IgG, Biotinylated
A00233-01-50B
50 ug
350.00
Anti Neutrophil Gelactinase Associated Lipocalin (NGAL)  (Human) Monoclonal Antibody A00233-05-100 100 ug 260.00
Anti Neutrophil Gelactinase Associated Lipocalin (NGAL)  (Human) Monoclonal Antibody A00233-06-100 100 ug 260.00
Anti Neutrophil Gelactinase Associated Lipocalin (NGAL)  (Human) Monoclonal Antibody A00233-07-100 100 ug 260.00
Anti Neutrophil Gelactinase Associated Lipocalin (NGAL)  (Human) Monoclonal Antibody A00233-08-100 100 ug 260.00
Anti Neutrophil Gelactinase Associated Lipocalin (NGAL)  (Rat) IgG
100 ug
220.00
Anti Neutrophil Gelactinase Associated Lipocalin (NGAL) (Rat)IgG, Biotinylated
A00233-02-50B
50 ug
350.00
Anti Neutrophil Gelactinase Associated Lipocalin (NGAL)  (Rat) Monoclonal Antibody A00233-09-100 100 ug 260.00
Anti Neutrophil Gelactinase Associated Lipocalin (NGAL)  (Rat) Monoclonal Antibody A00233-10-100 100 ug 260.00