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Stathmin/Metabolastin Recombinant, Antibody & ELISA Kits
 
BACKGROUND: Distal esophageal adenocarcinoma is a highly aggressive neoplasm. Despite advances in diagnosis and therapy, the prognosis is still poor. Stathmin (STMN-1) is a ubiquitously expressed microtubule destabilizing phosphoprotein. It promotes the disassembly of microtubules and prevents assembly. STMN-1 can cause uncontrolled cell proliferation when mutated and not functioning properly. Recently, found to be overexpressed in many types of human cancers. However, its clinical significance remains elusive in distal esophageal adenocarcinoma. Here, we reported for the first time that STMN-1 is highly overexpressed in adenocarcinomas of the distal esophagus and strongly associated with lymph node metastasis.
METHODS: STMN-1 expression in 63 cases of distal esophageal adenocarcinoma was analyzed by immunoblotting, while expression in esophageal adenocarcinoma cells was determined by immunocytochemistry, immunofluorescence, qRT-PCR and western blotting. Lentivirus-mediated RNAi was employed to knock-down STMN-1 expression in Human esophageal adenocarcinoma cells. The relationship between STMN-1 expression and lymph node metastasis in distal esophageal adenocarcinoma was determined by univariate and multivariate analyses.
RESULTS: STMN-1 was detected in 31 (49.21%) of the 63 cases. STMN-1 was highly overexpressed in specimens with lymph node metastasis pN (+), but its expression was almost undetected in pN (-) status. Multivarian regression analysis demonstrated that STMN-1 overexpression is an independent factor for lymph node metastasis in distal esophageal adenocarcinoma. STMN-1 shRNA effectively reduced STMN-1 expression in esophageal adenocarcinoma cells (P < 0.05), which significantly suppressed proliferation (P < 0.05), increased migration (P < 0.05) and invasion ability (P < 0.05) and G1 phase arrest (P < 0.05) which lead to induction of apoptosis in esophageal adenocarcinoma cells in vitro. To verify the in vitro data, we conducted in vivo tumor xenograft studies. Esophageal adenocarcinoma cells stably transfected with STMN-1 shRNA significantly reduced tumor xenografts volume in vivo.
CONCLUSIONS:STMN-1 overexpression is associated with lymph node metastasis and increase malignancy in distal esophageal adenocarcinoma. In vivo and in vitro laboratory findings, suggests that STMN-1 may be a suitable target for future therapeutic strategies in distal esophageal adenocarcinoma.

Human Stathmin/ Metablastin ELISA Kit
Code No.: SK00593-01
Size: 96 T
Price: $560.00 USD
Standard Range: 3.125-200 ng/ml
Sensitivity: 500 pg/ml
Specificity: Human
Calibration: Human Stathmin 1 Rec
Sample Type:serum, plasma
Intra-CV: 4-6%
Inter-CV: 8-14%
Protocol: PDF
ALWAYS REFER TO LOT SPECIFIC PROTCOL PROVIDED WITH EACH KIT FOR INSTRUCTIONS.

Human Stathmin/ Metablastin ELISA Kit
Code No.: SK00593-08
Size: 96 T
Price: $590.00 USD
Standard Range: 2.5-160 ng/ml
Sensitivity: 300 pg/ml
Specificity: Human
Calibration: Human Stathmin 1 Rec
Sample Type:serum, plasma
Intra-CV: 4-6%
Inter-CV: 8-14%
Protocol: PDF
ALWAYS REFER TO LOT SPECIFIC PROTCOL PROVIDED WITH EACH KIT FOR INSTRUCTIONS.

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Human Stathmin Recombinant
Code No.: 00593-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID:NP_981946
Gene ID: NM_203401
MW: 21 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF

 

Name
Code No.
Size
Price ($)
Stathmin/Metabolastin (Human) ELISA Kit
SK00593-08
96 T
590.00
Stathmin/Metabolastin (Human) ELISA Kit
96 T
560.00
Stathmin/Metabolastin (Human) ELISA Kit (480T)
480 T
2700.00
Stathmin/Metabolastin (Human) rec
50 ug
250.00
Stathmin/Metabolastin (Human) rec
100 ug
360.00
Stathmin/Metabolastin (Human) rec
00593-01-1000
1 mg
3300.00
Stathmin/Metabolastin (Human) rec Biotinylated
50 ug
360.00
Rabbit Anti Stathmin/Metabolastin (Human) Antibody
100 ug
260.00
Anti Stathmin/Metabolastin (Human) IgG Biotinylated
50 ug
360.00
Anti Stathmin/Metabolastin (Human) Monoclonal Antibody
A00593-04-100
100 ug
450.00
Anti Stathmin/Metabolastin (Human) Monoclonal Antibody Biotinylated
A00593-04-25B
25 ug
450.00
Anti Stathmin/Metabolastin (Human) Monoclonal Antibody
A00593-05-100
100 ug
450.00
Anti Stathmin/Metabolastin (Human) Monoclonal Antibody Biotinylated
A00593-05-25B
25 ug
450.00
Anti Stathmin/Metabolastin (Human) Monoclonal Antibody
A00593-06-100
100 ug
450.00
Anti Stathmin/Metabolastin (Human) Monoclonal Antibody Biotinylated
A00593-06-25B
25 ug
450.00