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Soluble Vascular Cell Adhesion Molecule-1 (sVCAM-1) /CD106 ELISA KIT

Higher Serum Levels of Soluble Intracellular Cell Adhesion Molecule-1 and Soluble Vascular CellAdhesion Molecule Predict Peripheral Artery Disease in Hemodialysis Patients

Background:  Serum levels of soluble intracellular cell adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion molecule-1 (sVCAM), and monocyte chemotactic protein 1 (MCP-1), are elevated in patients with peripheral artery disease (PAD). However, the levels of these cell adhesionmolecules in patients undergoing hemodialysis (HD) are unclear. Method:  A total of 112 HD patients were included and PAD was diagnosed using the ankle-brachial index and Doppler ultrasound. Serum levels of sICAM-1, sVCAM-1, and MCP-1 were assayed using ELISA. Results:  Out of 106 HD patients, 31 (27.7%) were diagnosed with PAD. After adjusting for risk factors, higher serum levels of sVCAM-1 and sICAM-1 were associated with PAD in HD patients, with an odds ratio of 5.3 (95% CI 3.3-65.5) and 2.7 (95% CI 1.2-21.8) respectively. Using sVCAM-1 and sICAM-1 for diagnosis of PAD in HD patients, sVCAM-1 had a sensitivity of 72.4% and specificity of 62.3% for sVCAM-1 and sICAM-1 had a sensitivity of 89.3% and a specificity of 40%. MCP-1 was not associated with PAD in HD patients. In addition, the fistula of HD patients with PAD had a lower A-V access flow. Conclusion:  sVCAM-1 and sICAM-1 was associated with higher risk of PAD in HD patients. Moreover, HD patients with PAD had a lower blood flow and lower A-V access flow. Our results showed that sVCAM-1 and sICAM-1 may be used as a screening markers for PAD in HD patients. 
Cheng CH, , et al. Nephrology (Carlton). 2012 Aug 21. doi: 10.1111/j.1440-1797.2012.01654.x. [Epub ahead of print]

Transient Variations in the Serum Concentrations of Cell Adhesion Molecules Following Retroperitoneal Laparoscopic and Open Radical Nephrectomy for Localized Renal-Cell Carcinoma

Abstract Purpose: To evaluate differences in the serum concentrations of cell adhesion molecules (CAMs) after retroperitoneal laparoscopic and conventional open radical nephrectomies for localized renal-cell carcinoma (RCC). Patients and Methods: A total of 62 patients with stage T(1)N(0)M(0) RCC were randomized to either a retroperitoneal laparoscopic radical nephrectomy group (n=31) or an open group (n=31). Serum levels of soluble cluster of differentiation 44 splice variant 6 (sCD44v6), soluble intercellular adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion molecule-1 (sVCAM-1), and soluble epithelial cadherin (sE-cadherin) were determined independently by enzyme linked immunosorbent assay (ELISA) preoperatively, and on postoperative days 1 and 5. In addition, follow-up results were compared. Results: On postoperative day 1, sCD44v6, sICAM-1, and sVCAM-1 levels increased significantly compared with preoperative levels in both groups (P<0.05). sE-cadherin levels decreased compared with preoperative levels in both groups without statistically significant differences (P>0.05). sCD44v6 levels in the retro-laparoscopy group were significantly higher than in the open group (P<0.05), while sICAM-1, sVCAM-1, and sE-cadherin levels showed no statistically significant differences between both groups (P>0.05). On postoperative day 5, all parameters in both groups were similar to preoperative values (P>0.05). Follow-up ranged from 7 to 18 months postoperatively in all 62 patients, with a 100% cancer-specific survival rate in each group. Conclusion: Although postoperatively higher serum concentrations of CAMs in both groups and significantly elevated sCD44v6 in the retro-laparoscopy group may be facilitated, the differences in CAMs between both groups are small and transient. Together with the similar follow-up results, this further supports previous studies that failed to show a difference in the oncologic outcomes between open and laparoscopic radical nephrectomy and provides a probable molecular mechanism.
Yang L,  et al.. J Endourol. 2012 Aug 20. [Epub ahead of print] .
vcam-1 elisa kit enables to detect circulating samples
   
Human Soluble VCAM-1/CD106 ELISA Kit
Code No.: SK00251-01
Size: 96 T
Price: $360.00 USD
Standard Range:15.6-1000 p g/mL
Sensitivity: 7.88 pg/ml
Sample Type: cell cultures, serum, plasma
Dilution Factor: 1000
Sample require: 10 uL
Intra-CV: 4-8%
Inter-CV: 6-10
Protocol: PDF
 
Human VCAM-1 Rec. (HEK293)
Code No.: 00251-02-10
Size: 10 ug
Price: $150.00 USD
Protein ID:NP_001069
Sequence:Phe25-Pro697
MW: 110-130 KD
Tag: His Tag on C-Terminus
Expressed: HEK293 cells
Purity: 95%
Data Sheet: PDF
Name
Code No.
Size
Price ($)
Soluble VCAM-1 (Human) ELISA Kit
96 T
360.00
Soluble VCAM-1 (Human) rec (HEK293)
00251-02-10
10 ug
150.00
Soluble VCAM-1 (Human) rec (HEK293)
00251-02-100
100 ug
460.00
Soluble VCAM-1 (Human) rec (HEK293)
00251-02-1000
1 mg
3600.00