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Visfatin ELISA Kit, Recombinant Protein, Antibody
PBEF1/NAMPT ELISA Kit, Recombinant, Antibody
A Biomarker for Diabetes, Cardiovascular Diseases

Alternative name:

  • PBEF1
  • NAMPT
Circulating Visfatin was measured by Dr. Pavlatou MG using Aviscera Bioscience's Human Visfatin ELISA Kit  on following paper.

Circulating cortisol-associated signature of glucocorticoid-related gene expression in subcutaneous fat of obese subjects

Objective: Serum cortisol concentrations fluctuate in a circadian fashion, and glucocorticoids exert strong effects on adipose tissue and induce obesity through the glucocorticoid receptor.
Design and Methods:To examine the impact of physiologic levels of circulating cortisol on subcutaneous adipose tissue, 25 overweight and obese subjects were employed, and their serum levels of morning (AM) and evening (PM) cortisol, AM/PM cortisol ratios, and 24-h urinary-free cortisol (UFC) were compared with their clinical parameters, serum cytokine levels, and mRNA expression of 93 receptor action-regulating and 93 glucocorticoid-responsive genes in abdominal subcutaneous fat.
Results and Conclusions:AM cortisol levels did not correlate with mRNA expression of the all genes examined, whereas PM cortisol levels, AM/PM cortisol ratios, and 24-h UFC were associated with distinct sets of these genes. Body mass index did not significantly correlate with the four cortisol parameters employed. These results suggest that physiologic levels of AM serum cortisol do not solely represent biological effects of circulating cortisol on the expression of glucocorticoid-related genes in subcutaneous adipose tissue, whereas PM levels, amplitude, and net amounts of the diurnally fluctuating serum cortisol have distinct effects. Through the genes identified in this study, glucocorticoids appear to influence intermediary metabolism, energy balance, inflammation, and local circadian rythmicity in subcutaneous fat. Our results may also explain in part the development of metabolic abnormality and obesity in subjects under stress or patients with melancholic/atypical depression who demonstrate elevated levels of PM serum cortisol.
Pavlatou MG et al. Obesity (Silver Spring). 2013 May;21(5):960-7. doi: 10.1002/oby.20073.
Human Visfatin Recombinant 00121-01-100 from Aviscera Bioscience (AVISCERA BIOSCIENCE ,INC FORMALLY KNOWN AS ADIPO BIOSCIENCE,INC) was used by Dr. Xiao J on following paper.

A novel adipocytokine visfatin protects against H2O2-induced myocardial apoptosis: A missing link between obesity and cardiovascular disease

Fat accumulation in obese individuals worsens the clinical outcomes of cardiovascular disease (CVD). Paradoxically, increased circulating adipocytokines secreted from visceral fat may confer cardioprotective effects. Visfatin, a novel adipocytokine, has anti-diabetic, anti-tumor, and pro-inflammatory properties. However, its effects on cardiomyocytes and the underlying mechanisms remain unknown. This article demonstrated that visfatin counteracted H2O2-induced apoptotic damage in H9c2 cardiomyocytes in a time-dependent manner. Qualitative immunofluorescence approaches demonstrated that visfatin pretreatment attenuated H2O2-induced DNA fragmentation (TdT-mediated dUTP-biotin nick end-labeling), phosphatidyl serine exposure (Annexin V/PI staining), and mitochondrial membrane potential (ΔΨm) depolarization (JC-1 staining). Biochemical studies on cardiomyoctes showed improved cell viability and reduced caspase-3 activation caused by visfatin pretreatment. Visfatin did not inhibit the death receptor-dependent apoptotic pathways, as characterized by its absence in both Fas and TNFR1 down-regulation. Instead, visfatin specifically suppressed the mitochondria-dependent apoptotic pathways, as characterized by changed levels of p53 and its downstream Bcl-2 family genes. Visfatin also up-regulated the protein levels of phosphorylated AMPK, and the anti-apoptotic action of visfatin was attenuated by the AMPK-specific inhibitor compound C. These results suggested that visfatin plays a critical role in cardioprotection by suppressing myocardial apoptosis via AMPK activation. These findings may be the missing link between obesity and CVD
Xiao J et al, J Cell Physiol. 2013 Mar;228(3):495-501. doi: 10.1002/jcp.24257.
 
Human Visfatin ELISA Kit
Code No.: SK00121-09
Size: 96 T
Price: $420.00 USD
Standard range: 1-64 ng/ml
Sensitivity: 100 pg/ml
Sample Type: Serum, EDTA plasma
Specificity: Human
Intra-CV: 4-8%
Inter-CV: 8-12%
Protocol: PDF
  Human Visfatin ELISA Kit
Code No.: SK00121-06
Size: 96 T
Price: $420.00 USD
Standard range: 0.25-32 ng/ml
Sensitivity: 50 pg/ml
Sample Type: Serum, EDTA plasma
Specificity: Human
Intra-CV: 4-8%
Inter-CV: 8-12%
Protocol: PDF
Human Visfatin Recombinant
Code No.: 00121-01-100
Size: 100 ug
Price: $300.00 USD
Protein ID:P43490
Gene ID: 10135
MW:59KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
Rat Visfatin Recombinant
Code No.: 00121-02-100
Size: 100 ug
Price: $300.00 USD
Protein ID:Q80Z29
Gene ID: 297508
MW:59KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF
Anti visfatin (Human) IgG
Code:A00121-01-100

Size: 100 ug
Ab Type: Polyclonal IgG
Purification: Protein A
Host: Rabbit
Antigen: visfatin (Human), rec
Positive tissues:  human adipose tissues and kidney tumor tissues
Working Conc.: 1-2 ug/ml for WB, 2-4 ug/ml for IHC
Blocking: 10% non-fat dried milk
More Info: PDF
Anti visfatin (Human) IgG
Code:A00121-01-100

Size: 100 ug
Ab Type: Polyclonal IgG
Purification: Protein A
Host: Rabbit
Antigen: visfatin (Human), rec
Positive tissues:  human adipose tissues and kidney tumor tissues
Working Conc.: 1-2 ug/ml for WB, 2-4 ug/ml for IHC
Blocking: 10% non-fat dried milk
More Info: PDF
Anti visfatin (Human) IgG
Code:A00121-01-100

Size: 100 ug
Ab Type: Polyclonal IgG
Purification: Protein A
Host: Rabbit
Antigen: visfatin (Human), rec
Positive tissues:  human adipose tissues and kidney tumor tissues
Working Conc.: 1-2 ug/ml for WB, 2-4 ug/ml for IHC
Blocking: 10% non-fat dried milk
More Info: PDF
anti rat visfatin IgG A00121-02-100 from aviscera bioscience Anti visfatin (Rat) IgG
Code:A00121-02-100

Size: 100 ug
Ab Type: Polyclonal IgG
Purification: Protein A
Host: Rabbit
Antigen: visfatin (Rat), rec
Positive tissues:  rat  or mouse adipose tissues
Working Conc.: 2-4 ug/ml for WB, 5 ug/ml for IHC
Blocking: 10% non-fat dried milk
More Info: PDF
   

 

 

Name
Code No.
Size
Price ($)
Human Visfatin ELISA Kit
96 T
420.00
Human Visfatin ELISA Kit
2 x 96 T
800.00
Human Visfatin ELISA Kit
5 x 96 T
1800.00
Human Visfatin ELISA Set (15 x 96 T)
SK00121-07-15
96 T
860.00
Human Visfatin Recombinant 00121-01-100 100 ug 300.00
Rat Visfatin Recombinant 00121-02-100 100 ug 300.00
Anti Human Visfatin IgG A00121-01-100 100 ug 220.00
Anti Rat Visfatin IgG A00121-02-100 100 ug 220.00