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Autotaxin/ENPP2 ELISA Kit
A biomarker for mabolic syndrome, diabetes and cancer Research

ENPP2 contributes to adipose tissue expansion in diet-induced obesity

Body weight is tightly regulated by food intake and energy dissipation, and obesity is related to decreased energy expenditure (EE). Herein, we show that nucleotide pyrophosphatase/phosphodieseterase 2 (ENPP2, autotaxin) is an adipose-derived, secreted enzyme that controls adipose expansion, brown adipose tissue (BAT) function, and EE. In mice, Enpp2 was highly expressed in visceral white adipose tissue and BAT, and is downregulated in hypertrophied adipocytes/adipose tissue. Enpp2+/- mice and adipocyte-specific Enpp2 knockout mice fed a high-fat diet showed smaller body weight gains and less insulin resistance than control mice fed the same diet. BAT was functionally more active, and EE was increased in Enpp2-deficient mice. In humans, ENPP2 expression in subcutaneous fat and ENPP2 levels in serum were reduced in obese subjects. Taken together, our results establish ENPP2 as an adipose-derived, secreted enzyme that regulates adipose obesity and systemic metabolism. They also suggest ENPP2 could be a useful therapeutic target for the treatment of metabolic disease.
Nishimura S et al. Diabetes. 2014 Jun 26. pii: DB_131694. [Epub ahead of print]
Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection.

1: Kadekar S, et al.Exocrine pancreatic carcinogenesis and autotaxin expression. PLoS One. 2012;7(8):e43209. doi: 10.1371/journal.pone.0043209
2: Brindley DN, et al. Role of the autotaxin-lysophosphatidate axis in cancer resistance to chemotherapy and radiotherapy. Biochim Biophys Acta. 2013 Jan;1831(1):74-85.
3: Abu El-Asrar AM, et al. Expression of lysophosphatidic acid, autotaxin and acylglycerol kinase as
biomarkers in diabetic retinopathy. Acta Diabetol. 2013 Jun;50(3):363-71.
4: Ikeda H, Yatomi Y. Autotaxin in liver fibrosis. Clin Chim Acta. 2012 Nov 20;413(23-24):1817-21.
5: Oikonomou N, et al. Pulmonary autotaxin expression contributes to the pathogenesis of pulmonary fibrosis. Am J Respir Cell Mol Biol.2012 Nov;47(5):566-74.
6: Nakamura K,et al. Serum autotaxin is not a useful biomarker for ovarian  cancer. Lipids. 2012 Sep;47(9):927-30.
7: Wood NJ. Liver: Potential of autotaxin as a diagnostic marker and therapeutic target for itch of cholestasis. Nat Rev Gastroenterol Hepatol. 2012 May 1;9(6):303.
8: Kremer AE, et al. Serum autotaxin is increased in pruritus of cholestasis, but not of other origin, and responds to therapeutic interventions. Hepatology. 2012 Oct;56(4):1391-400
9: Houben AJ, Moolenaar WH. Autotaxin and LPA receptor signaling in cancer.
Cancer Metastasis Rev. 2011 Dec;30(3-4):557-65.
10: Dohi T, et al. Increased circulating plasma lysophosphatidic acid in patients with acute coronary syndrome. Clin Chim Acta. 2012 Jan 18;413(1-2):207-12.
11: Moolenaar WH, Perrakis A. Insights into autotaxin: how to produce and present
a lipid mediator. Nat Rev Mol Cell Biol. 2011 Sep 14;12(10):674-9.
12: Masuda A, et al. Serum autotaxin measurements in pregnant women: application for the differentiation of normal pregnancy and pregnancy-induced hypertension. Clin Chim Acta. 2011 Oct 9;412(21-22):1944-50.

 
human ENPP2 samples assay by SK00526-06
human ENPP-2 ELISA Kit SK00526-06 Human Autotaxin/ENPP2 ELISA Kit
Code No.: SK00526-06
Size: 96 T
Price: $460.00
Standard range:0.5-32 ng/ml
Sensitivity:100 pg/ml
Sample Type:serum, plasma
Dilution factor: 40 for plasma and serum
Intra-CV: 4-6%
Inter-CV: 8-12%
Data Sheet: PDF
Mouse/Rat Autotaxin/ENPP2 ELISA Kit
Code No.: SK00526-01
Size: 96 T
Price: $460.00
Standard range:0.5-32 ng/ml
Sensitivity:100 pg/ml
Sample Type:serum
Sample require: 100 ul per well
Intra-CV: 4-6%
Inter-CV: 8-12%
Data Sheet: PDF

 

Name
Code
Size
Price ($)

Autotaxin/ENPP2 (Human) ELISA Kit

96 T

460.00

Autotaxin/ENPP2 (Mouse/Rat) ELISA Kit

96 T

460.00

Autotaxin/ENPP2 (Human) Rec (HEK293 cells derived)

00526-02-5

5 ug

250.00

Autotaxin/ENPP2 (Human) Rec (HEK293 cells derived)

10 ug

340.00

Autotaxin/ENPP2 (Mouse) Rec (HEK293 cells derived)

00526-04-5

5 ug

250.00

Autotaxin/ENPP2 (Mouse) Rec (HEK293 cells derived)

10 ug

340.00