h
aviscera bioscience  
AVISCERA BIOSCIENCE  
line decor
  HOME  ::  
line decor
   
 

Mesothelin & MPF ELISA, Antibody, Proteins

Because mesothelioma initially progresses on the surface of the pleura and peritoneum without forming masses, it has been difficult to diagnose at an early stage. It would be very useful to identify a tumor marker that could be used for screening to enable more diagnoses to be made at an early, treatable stage.
MATERIALS AND METHODS: We had previously identified N-ERC/mesothelin as a potential biomarker for mesothelioma. In the current work, we used a newly developed ELISA system to gain data on N-ERC/mesothelin levels in various clinical settings. A total of 102 healthy volunteers were recruited. In addition, 39 patients were diagnosed with mesothelioma, 53 patients were diagnosed with diseases that should be distinguished from mesothelioma, and 201 subjects were diagnosed with asbestos-related nonmalignant diseases (including simple exposure to asbestosis) who were treated at any of the cooperating hospitals were enrolled.
RESULTS: Serum N-ERC/mesothelin levels measured by a new ELISA system showed that the median values from patients with mesothelioma were extremely high compared with levels obtained from other patients. Analysis in terms of histologic type showed that serum levels of N-ERC/mesothelin were elevated in epithelioid type mesothelioma, especially. In four important models of clinical settings, the sensitivity and specificity of N-ERC/mesothelin were about 71% to 90% and 88% to 93%, respectively.
CONCLUSION: N-ERC/mesothelin is a very promising tumor marker for mesothelioma, especially epithelioid mesothelioma.
Higashiyama M, Ito T, Tanaka E, Shimada Y. Ann Surg Oncol. 2007 Dec;14(12):3419-27. Epub 2007 Sep 26.
BACKGROUND: Knowledge of the characteristics and effective use of tumour markers for mesothelioma is essential for early-stage diagnosis of mesothelioma. We examined whether renal dysfunction influences blood concentrations of promising new tumour markers, N-ERC/mesothelin (N-ERC) and osteopontin (OPN), to an important degree.
PATIENTS AND METHODS: Levels of serum N-ERC and plasma OPN in 32 patients with chronic renal dysfunction, 22 of whom were on hemodialysis (CKD group), and 102 healthy volunteers were measured.
RESULTS: Serum concentrations of N-ERC and plasma concentrations of OPN in the CKD group were significantly higher than those in volunteers, regardless of diabetes status and age. Blood concentrations of these markers increased as renal function decreased.
CONCLUSION: N-ERC and OPN concentrations are significantly influenced by renal function. Therefore, the extent of renal failure must be considered when inferring the existence of tumours and chemotherapeutic response from the values of these markers in routine practice.
Shiomi K,, et al. Clin Cancer Res. 2008 Mar 1;14(5):1431-7.
 
mesothelin
 
human MPF elisa kit from aviscera bioscience
 
 
   
   
Human Megakaryocyte-potentiating factor (MPF) High-Sensitivity ELISA Kit
Code No.: SK00722-08
Size: 96 T
Standard Range:125-4000 pg/ml
Sensitivity:20 pg/ml
Calibration: Glycosylated MPF (Human) (human cell derived)
Specificity: the Glycosylated human MPF/MSLN (37-286). Validated antibody by western blot.
Sample Type: serum, plasma
Sample requres: 100uL per well,
Specificity: human MPF
IntraCV: 4-6%
InterCV: 8-10%
Protocol: PDF
Human Megakaryocyte-potentiating factor (MPF) ELISA Kit
Code No.: SK00722-06
Size: 96 T
Standard Range:250-16000 pg/ml
Sensitivity:75 pg/ml
Calibration: Glycosylated MPF (Human) (human cell derived)
Specificity: the Glycosylated human MPF/MSLN (37-286). Validated antibody by western blot.
Sample Type: serum, plasma
Sample requres: 100uL per well,
Specificity: human MPF
IntraCV: 4-6%
InterCV: 8-10%
Protocol: PDF
Human Megakaryocyte-potentiating factor (MPF) ELISA Kit
Code No.: SK00722-07
Size: 96 T
Standard Range:0.5- 32 ng/ml
Sensitivity:100 pg/ml
Calibration: Glycosylated MPF (Human) (human cell derived)
Specificity: the Glycosylated human MPF/MSLN (37-286). Validated antibody by western blot.
Sample Type: serum, plasma
Sample requres: 100uL per well,
Specificity: human MPF
IntraCV: 4-6%
InterCV: 8-10%
Protocol: PDF
Anti Human MPF Monoclonal Antibody
Code No.:A00722-10-100
Size: 100 ug
Clone: 1A2
Antibody Type: Monoclonal
Purification: Protein G
Immunogen: Human MPF (HEK293 derived)
Application: WB, E
Data Sheet
Protocol: PDF
immunostaining of A00722-17-100 Anti MPF (Human) Monoclonal Antibody
Code No.:A00722-17-100
Size: 100 ug
Clone:
Host: Mouse
Immunogen: Human MPF Recombinant (HEK293)
Purification: Protein G Affinity
Target Protein ID:Q13421-1
Target Gene ID:10232
Application: ELISA (Capture (2 ~4 ug/mL)
IHC (4 ~ 8 ug/mL)
Carry: BSA free
Formulation:Dried
Storage: -20 ~ -70 C.
Protocol: PDF
immunostaining lung cancer tissues by anti MPF (Human) Monoclonal antibody A00722-16-100 Anti MPF (Human) Monoclonal Antibody
Code No.:A00722-16-100
Size: 100 ug
Clone:
Host: Mouse
Immunogen: Human MPF Recombinant (HEK293)
Purification: Protein G Affinity
Target Protein ID:Q13421-1
Target Gene ID:10232
Application: ELISA (Capture (4 ug/mL)/ Detection(1~2 ug/mL)
IHC (4 ~ 8 ug/mL) (Antigen retrieval require, the paraffin embedded human lung cancer tissues )
WB (2-4 ug/mL)
Carry: BSA free
Formulation:Dried
Storage: -20 ~ -70 C.
Protocol: PDF
  Human mesothelin Recombinant
Code No.:00722-01-100
Size: 100 ug
Protein ID:Q13421-1
Gene ID:10232
MW: 51.9 KDa
Tag: His tag om N-terminus
Expression:E. Coli -derived
Purity: 90%
Protocol: PDF
Human Mesothelin (MSLN) Recombinant
Code No.:00722-03-10
Size: 10 ug
Price: $120.00 USD
Protein ID:NP_005814
Gene ID:NM_005823
MW: 42 KDa
Tag: His tag om C-terminus
Expression:Human Cells HEK293 -derived
Purity: 95%
Protocol: PDF
Human Megakaryocyte-potentiating factor (MPF) Recombinant
Code No.:00722-02-100
Size: 100 ug
Protein ID:Q13421-1
Gene ID:10232
MW: 30.5 KDa
Tag: His tag om N-terminus
Expression:E. Coli -derived
Purity: 95%
Protocol: PDF
Human Megakaryocyte-potentiating factor (MPF) Recombinant
Code No.:00722-06-10
Size: 10 ug
Protein ID:NP_005814
Gene ID:NM_005823
MW: 33 KDa
Tag: His tag om C-terminus
Expression:Human Cells HEK293 -derived
Purity: 95%
Protocol: PDF
   
Anti Mesothelin (Human) IgG
Code No.:A00722-01-100
Size: 100 ug
Host: Rabbit
Immunogen: Mesothelin (Human)
Antibody Type: polyclonal
Purification: Protein A affinity
Formulation: Lyophilized PBS without carry protein
Application: IHC (2-4 ug/ml);
                      WB (2-4 ug/ml)
                      ELISA (1 ug/ml)
Protocol: PDF
anti mesothelin antibody Anti Mesothelin (Human) IgG
Code No.:A00722-01-100
Size: 100 ug
Host: Rabbit
Immunogen: Mesothelin (Human)
Antibody Type: polyclonal
Purification: Protein A affinity
Formulation: Lyophilized PBS without carry protein
Application: IHC (2-4 ug/ml);
                      WB (2-4 ug/ml)
                      ELISA (1 ug/ml)
Protocol: PDF

Order MPF & Mesothelin Products online and checkout

 

Name
Code No.
Size
Megakaryocyte-potentiating Factor (MPF) (Human) ELISA Kit
96 T
Megakaryocyte-potentiating Factor (MPF) (Human) ELISA Kit
96 T
Megakaryocyte-potentiating Factor (MPF) (Human) ELISA Kit
SK00722-07
96 T
Mesothelin (Human) ELISA Kit
96 T
Megakaryocyte-potentiating Factor (MPF) (Human) Rec. 00722-02-100 100 ug
Megakaryocyte-potentiating Factor (MPF) (Human) Rec., Biotin Conjugated 00722-02-50B 50 ug
Megakaryocyte-potentiating Factor (MPF) (Human) Rec. (Human HEK293 derived) 00722-06-10 10 ug
Megakaryocyte-potentiating Factor (MPF) (Human) Rec. (Human HEK293 derived) 00722-06-50 50 ug
Megakaryocyte-potentiating Factor (MPF) (Human) Rec. (Human HEK293 derived) 00722-06-50 1 mg
Human Mesothelin Recombinant 00722-01-50 50 ug
Human Mesothelin Recombinant 00722-01-100 100 ug
Anti Mesothelin (Human) IgG A00722-01-100 100 ug
Anti Mesothelin (Human) IgG Biotin Conjugated A00722-01-50B 50 ug
Anti Mesothelin (Human) IgG Cy3 Conjugated A00722-01-50C3 50 ug
Human Mesothelin rec (HEK293 derived) 00722-03-10 10 ug
Human Mesothelin rec (HEK293 derived) 00722-03-50 50 ug
Human Mesothelin rec (HEK293 derived) 00722-03-100 100 ug
Human Mesothelin rec (HEK293 derived) 00722-03-1000 10 x 100 ug