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Renin ELISA Kit & Angiotensinogen ELISA Kit
Angiotensin I Converting Enzyme (ACE) ELISA Kit
Three Biomarkers for Hypertension

Role of collecting duct renin in blood pressure regulation

Numerous studies indicate that renin is synthesized and secreted by the collecting duct. Collecting duct-derived renin may act directly upon intercalated and/or principal cells through direct interaction with prorenin receptors and/or through cleavage of proximal tubule-derived angiotensinogen to ultimately produce angiotensin II and activate AT1 receptors. Preliminary studies suggest that the net effect of CD renin would be to increase distal nephron salt reabsorption and increase blood pressure. Collecting duct renin production is markedly increased in diabetes and angiotensin-II induced hypertension, suggesting that this system may exert pathophysiological effects. In this brief review, we summarize the current literature on synthesis and regulation of CD renin and consider potential mechanisms by which it regulates blood pressure.
Ramkumar N, Kohan DE. Am J Physiol Regul Integr Comp Physiol. 2013 May 1. [Epub ahead of print]

The collecting duct is the major source of prorenin in diabetes

In addition to the juxtaglomerular apparatus, renin is also synthesized in renal tubular epithelium, including the collecting duct (CD). Angiotensin (Ang) II differentially regulates the synthesis of juxtaglomerular (inhibition) and CD (stimulation) renin. Because diabetes mellitus, a disease with high intrarenal renin-Ang system and Ang II activity, is characterized by high prorenin levels, we hypothesized that the CD is the major source of prorenin in diabetes. Renin granular content was visualized using in vivo multiphoton microscopy of the kidney in diabetic Munich-Wistar rats. Diabetes caused a 3.5-fold increase in CD renin, in contrast to less pronounced juxtaglomerular changes. Ang II type 1 receptor blockade with Olmesartan reduced CDrenin to control levels but significantly increased juxtaglomerular renin. Using a fluorogenic renin assay, the prorenin component of CD renin content was measured by assessing the difference in enzymatic activity of medullary homogenates before and after trypsin activation of prorenin. Trypsinization caused no change in control renin activity but a 5-fold increase in diabetes. Studies on a CD cell line (M1) showed a 22-fold increase inrenin activity after trypsinization and a further 35-fold increase with Ang II treatment. Therefore, prorenin significantly contributes to baseline CD renin. Diabetes, possibly via Ang II, greatly stimulates CD prorenin and causes hyperplasia of renin-producing connecting segments. These novel findings suggest that, in a rat model of diabetes, prorenin content and release from the CD may be more important than the juxtaglomerular apparatus in contrast to the existing paradigm.
Kang J.J., et al.Hypertension. 2008 Jun;51(6):1597-604

Plasma Renin and Cardiovascular Risk: What Is the Evidence for an Association?

Renin is a key component of the renin-angiotensin system (RAS), which plays an important role in the maintenance of blood pressure and electrolyte-volume homeostasis. RAS also plays a role in cardiovascular (CV) disease as a result of effects on inflammation and oxidative stress. There is growing evidence that plasma renin activity may be a marker of CV risk in hypertensive patients. This increase in CV risk likely reflects activation of the RAS as a whole. Patients undergoing treatment for hypertension experience a reactive increase in renin, especially if treated with diuretics, vasodilators or agents that block the RAS. There is not sufficient evidence, however, that this reactive increase in renin is intrinsically harmful for hypertensive patients in whom adequate levels of RAS blockade have been achieved. Indeed, in such patients, additional RAS blockade may not be beneficial and may even increase the risk of adverse events. Plasma renin may be an important prognostic indicator in untreated patients, and one that can be used to help in the choice of antihypertensive treatment. Currently, however, the link between plasma renin and CV risk in treated patients is inconsistent between different populations and in various clinical conditions, calling for further investigation.
Volpe M, Unger T. Cardiology. 2013 Apr 30;125(1):50-59. [Epub ahead of print]
  • renin elisa kit

human renin elisa kit research use from aviscera
Human Renin ELISA Kit
Code No.: SK00274-01
Size: 96 T
Price: $340.00
Standard range:125-8000 pg/ml
Sensitivity: 30 pg/ml
Specificity: Human Renin (Pro and Mature)(
Calibration: recombinant human Pro Renin (HEK293 cells derived)
Sample Type:serum, plasma
Sample require: 100 ul per well
Intra-CV: 4-6%
Inter-CV: 8-10%
Data Sheet: PDF
  Human Renin ELISA Kit (wide range)
Code No.: SK00274-06
Size: 96 T
Price: $460.00
Standard range:62.5-16000 pg/ml
Sensitivity: 30 pg/ml
Specificity: Human Renin (Pro and Mature)
Calibration: recombinant human Pro Renin (HEK293 cells derived)
Sample Type:serum, plasma, Urine
Sample require: 100 ul per well
Intra-CV: 4-6%
Inter-CV: 8-10%
Data Sheet: PDF
Human Angiotensinogen ELISA Kit
Code No.: SK00275-01
Size: 96 T
Price: $380.00
Standard range:156-10000 pg/ml
Sensitivity: 50 pg/ml
Sample Type:serum, plasma
Sample require: 100 ul per well
Intra-CV: 4-6%
Inter-CV: 8-10%
Data Sheet: PDF
Human Angiotensin I Converting Enzyme (ACE) ELISA Kit
Code No.: SK00290-01
Size: 96 T
Price: $380.00
Standard range:62.5-4000 pg/ml
Sensitivity: 10 pg/ml
Sample Type:serum, plasma
Sample require: 100 ul per well
Intra-CV: 4-6%
Inter-CV: 8-10%
Data Sheet: PDF

 

Name
Code No.
Size
Price ($)
Renin (Human) ELISA Kit
96 T
340.00
Renin (Human) ELISA Kit (wide range)
SK00274-06
96 T
460.00
Angiotensinogen (Human) ELISA Kit
96 T
380.00
Angiotensin I Converting Enzyme (ACE) (Human) ELISA Kit
96 T
340.00
Renin (Human) Rec (human cells derived) Biotinylated
00274-01-10B
10 ug
490.00
Renin (Human) Rec (human cells derived)
20 ug
320.00
Renin (Human) Rec (human cells derived)
50 ug
560.00
Renin (Human) Rec (human cells derived)
00274-02-1000
1 mg
inquire
Renin (Mouse) Rec (human cells derived)
00274-03-50
50 ug
660.00
Renin (Mouse) Rec (human cells derived)
00274-03-1000
1 mg
inquire
       
Angiotensinogen (Human) Rec.
00275-01-5
5 ug
150.00