adipobioscience  
AVISCERA BIOSCIENCE  
line decor
  HOME  ::  
line decor
   
 
Soluble Tumor Necrosis Factor Receptor 2 (sTNFR2)

Alternative name:

  • Tumor necrosis factor receptor superfamily member 1B
  • Tumor necrosis factor receptor type II
  • p80 TNF-alpha receptor
  • CD_antigen=CD120b

Behavior of Tumor Necrosis Factor-α and Tumor Necrosis Factor Receptor 1/Tumor Necrosis Factor Receptor 2System in Mononuclear Cells Recovered From Peritoneal Fluid of Women With Endometriosis at Different Stages

During endometriosis, a breakdown occurs in endometrial and peritoneal homeostasis caused by cytokine-induced cell proliferation and dysregulation of apoptosis. We studied tumor necrosis factor (TNF)-α, TNF receptor (TNFR) 1, and TNFR2 gene expression at both messenger RNA (mRNA) and protein levels in peritoneal fluid (PF) mononuclear cells (PFMCs), the percentages of these cells bearing the same markers, and soluble TNF-α (sTNF-α) values in PF of 80 women with endometriosis. We found that TNFR1 mRNA and protein levels, the percentages of TNFR1-bearing PFMCs, and sTNF-α values decreased from minimal to severe stages of the disease. Instead, TNF-α and TNFR2 mRNA and protein levels, the percentages of membrane TNF-α (mTNF-α)- and TNFR2-bearing PFMCs increased as the disease worsened. These data allow us to hypothesize that, in early stages, the high percentages of TNFR1-bearing PFMCs and the high levels of sTNF-α could address signal toward complex I pathway, favoring the inflammatory response. With the worsening of the disease, the low percentages of TNFR1-bearing PFMCs are probably due to decreased TNFR1 mRNA transcription and protein translation rate. In early stages (minimal and mild), the percentages of both TNFR2- and mTNF-α-bearing PFMCs are so low, due to decreased mRNA transcription and protein translation rate, that subsequent cellular events may depend minimally by this interaction. The high levels of sTNF-α may be rerouted to bind TNFR1. In contrast, in the moderate and severe stages, the high percentages of TNFR2-bearing PFMCs may be saturated by high percentages of mTNF-α-bearing PFMCs, triggering death process. So, in endometriosis, each component of the TNF-α/TNFRs system may trigger opposite cellular fate.
Salmeri FM et al. Reprod Sci. 2014 May 20. pii: 1933719114536472. [Epub ahead of print]

Circulating TNF Receptor 2 is Closely Associated with the Kidney Function in Non-Diabetic Japanese Subjects

Aim: Chronic kidney disease (CKD) is associated with cardiovascular events. Tumor necrosis factor (TNF) and/or its receptors have been postulated to be involved in renal pathophysiology. It is unclear whether an increased TNF system activity is present before the development of apparent CKD. Methods: Four hundred and twenty non-diabetic Japanese subjects with an estimated GFR (eGFR) greater than 60 ml/min/1.73 m2 were recruited for measurement of the HbA1c, insulin, TNF system activity (TNF-α, soluble TNF receptor 1 (sTNF-R1) and sTNF-R2) levels and various parameters, including the lipid, high-sensitivity C-reactive protein (hsCRP), high-molecular-weight (HMW) adiponectin and leptin levels. The subjects were stratified according to the eGFR: the G1 level (eGFR ≧90 ml/min/1.73 m2) and the G2 level (90 >eGFR ≧60 ml/min/1.73 m2). Results: Whereas no significant differences were observed in gender, body mass index (BMI), blood pressure, insulin, TNF-α, hsCRP, HMW adiponectin or leptin between the two groups, the values for age, HbA1c, triglycerides, sTNF-R1 and sTNF-R2 were significantly higher in the subjects with a G2 level of eGFR than in those with a G1 level. In contrast, the HDL cholesterol levels were significantly lower in the subjects with a G2 level than in those with a G1 level. Linear negative correlations were also observed between eGFR and age, BMI, HbA1c, triglycerides, sTNF-R1 and sTNFR2, respectively. A multiple logistic regression analysis revealed that only sTNF-R2 was associated with the presence of a G2 level of eGFR (Odds ratio 1.092, 95% CI 1.013-1.177, P=0.021). Conclusions: The circulating sTNF-R2 level is closely associated with the kidney function in non-diabetic Japanese subjects.
Kurashina T et al. J Atheroscler Thromb. 2014 Apr 8. [Epub ahead of print]

Human Soluble TNFR2 ELISA
Code No.: SK00221-02
Size: 96 T
Price: $360.00 USD
Standard Range:7.8-500 pg/ml
Dynamic Range: 7.8-500 pg/ml
Sensitivity:7.8g/ml
Sample Type: serum, EDTA plasma
Sample requres: 25 ul, 100 ul per well of 10 fold diluted samples
IntraCV: 6-8%
InterCV: 8-10%
Protocol: PDF
mouse soluble TNFR2 elisa kit from aviscera bioscience
Mouse Soluble TNFR2 ELISA
Code No.: SK00221-03
Size: 96 T
Price: $360.00 USD
Standard Range:9.4-600 pg/ml
Dynamic Range: 9.4-600 pg/ml
Sensitivity:4.9g/ml
Sample Type: serum, EDTA plasma
Sample requres: 25 ul, 100 ul per well of 10 fold diluted samples
IntraCV: 6-8%
InterCV: 8-10%
Protocol: PDF

 

Human Soluble TNFR1 ELISA
Code No.: SK00111-02
Size: 96 T
Price: $360.00 USD
Standard Range:12.5-800 pg/ml
Dynamic Range: 12.5-800 pg/ml
Sensitivity:12.5 g/ml
Sample Type: serum, EDTA plasma
Sample requres: 25 ul, 100 ul per well of 10 fold diluted samples
IntraCV: 6-8%
InterCV: 8-10%
Protocol: PDF

 

 

 

Name
Code No.
Size
Price ($)
Human Soluble TNF-R2ELISA Kit
96 T
360.00
Mouse Soluble TNF-R2 ELISA Kit
96 T
360.00