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Sortilin (Soluble) ELISA Kit
New Biomarker for Cardiovascular Diseases

Alternative name:

  • Glycoprotein 95
  • Neurotensin Receptor 3
  • SORT1
 
 
Genome-wide association studies (GWAS) have identified a genetic variant at a locus on chromosome 1p13 that is associated with reduced risk of myocardial infarction, reduced plasma levels of LDL cholesterol (LDL-C), and markedly increased expression of the gene sortilin-1 (SORT1) in liver.Sortilin is a lysosomal sorting protein that binds ligands both in the Golgi apparatus and at the plasma membrane and traffics them to the lysosome. We previously reported that increased hepatic sortilin expression in mice reduced plasma LDL-C levels. Here we show that increased hepatic sortilinnot only reduced hepatic apolipoprotein B (APOB) secretion, but also increased LDL catabolism, and that both effects were dependent on intact lysosomal targeting. Loss-of-function studies demonstrated that sortilin serves as a bona fide receptor for LDL in vivo in mice. Our data are consistent with a model in which increased hepatic sortilin binds intracellular APOB-containing particles in the Golgi apparatus as well as extracellular LDL at the plasma membrane and traffics them to the lysosome for degradation. We thus provide functional evidence that genetically increased hepaticsortilin expression both reduces hepatic APOB secretion and increases LDL catabolism, providing dual mechanisms for the very strong association between increased hepatic sortilin expression and reduced plasma LDL-C levels in humans.
Strong A et al. J Clin Invest. 2012 Aug 1;122(8):2807-16. doi: 10.1172/JCI63563. Epub 2012 Jul 2.
Recent GWAS have identified SNPs at a human chromosom1 locus associated with coronary artery disease risk and LDL cholesterol levels. The SNPs are also associated with altered expression of hepatic sortilin-1 (SORT1), which encodes a protein thought to be involved in apoB trafficking and degradation. Here, we investigated the regulation of Sort1 expression in mouse models of obesity. Sort1 expression was markedly repressed in both genetic (ob/ob) and high-fat diet models of obesity; restoration of hepatic sortilin-1 levels resulted in reduced triglyceride and apoB secretion. Mouse models of obesity also exhibit increased hepatic activity of mammalian target of rapamycin complex 1 (mTORC1) and ER stress, and we found that administration of the mTOR inhibitor rapamycin to ob/ob mice reduced ER stress and increased hepatic sortilin-1 levels. Conversely, genetically increased hepatic mTORC1 activity was associated with repressed Sort1 and increased apoB secretion. Treating WT mice with the ER stressor tunicamycin led to marked repression of hepatic sortilin-1 expression, while administration of the chemical chaperone PBA to ob/ob mice led to amelioration of ER stress, increased sortilin-1 expression, and reduced apoB and triglyceride secretion. Moreover, the ER stress target Atf3 acted at the SORT1 promoter region as a transcriptional repressor, whereas knockdown of Atf3 mRNA in ob/ob mice led to increased hepatic sortilin-1 levels and decreased apoB and triglyceride secretion. Thus, in mouse models of obesity, induction of mTORC1 and ER stress led to repression of hepatic Sort1 and increased VLDL secretion via Atf3. This pathway may contribute to dyslipidemia in metabolic disease.
Ai D, et al. J Clin Invest. 2012 May 1;122(5):1677-87. doi: 10.1172/JCI61248. Epub 2012 Apr 2.
human soluble sortilin elisa kit from www.aviscerabioscience.com
Human Sortilin ELISA
Code No.: SK00472-01
Size: 96 T
Price: $490.00 USD
Standard Range:1.56-200 ng/ml
Sensitivity:300 pg/ml
Sample Type: serum, plasma
Sample requres: 100uL per well
IntraCV:4-6%
InterCV: 8-12%
Protocol: PDF
  Mouse Sortilin ELISA
Code No.: SK00472-03
Size: 96 T
Price: $490.00 USD
Standard Range:1.56-200 ng/ml
Sensitivity:300 pg/ml
Sample Type: serum, plasma,
Sample requres: 100uL per well
IntraCV:4-6%
InterCV: 8-12%
Protocol: PDF
  Human Pro Neurotensin Fc (HEK293) Recombinant
Code No.: 00792-01-10
Size: 10 ug
Price: $260.00 USD
Protein ID:NP_006174.1
Gene ID: NM_006183.4
MW: 41 KD
Tag: Fc Tag on C-Terminus
Expressed: HEK293
Purity: 85-90%
Data Sheet: PDF

Ligands for Sortilin:

 

 

Name
Code No.
Size
Price ($)
Soluble Sortilin (Human) ELISA Kit
96 T
490.00
Soluble Sortilin (Mouse) ELISA Kit
96 T
490.00
Pro Neurotensin (Human) Fc (HEK293)
00792-01-10
10 ug
260.00
Pro Neurotensin (Human) Fc (HEK293)
00792-01-100
100 ug
inquire
Pro Neurotensin (Human) Fc (HEK293)
00792-01-1000
1 mg
inquire
Pro Neurotensin (Human) Fc (HEK293) Biotinylated
00792-01-20B
20 ug
420