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Human Endoplasmic Reticulum Resident Protein 18 (ERp18)/ Thioredoxin-like Protein 19 (TLP19)

Gene expression signature of non-involved lung tissue associated with survival in lung adenocarcinoma patients

Lung adenocarcinoma patients of similar clinical stage and undergoing the same treatments often have marked inter-individual variations in prognosis. These clinical discrepancies may be due to the genetic background modulating an individual's predisposition to fighting cancer. Herein, we hypothesized that the lung microenvironment, as reflected by its expression profile, may affect lung adenocarcinoma patients' survival. The transcriptome of non-involved lung tissue, excised from a discovery series of 204 lung adenocarcinoma patients, was evaluated using whole-genome expression microarrays (with probes corresponding to 28,688 well-annotated coding sequences). Genes associated with survival status at 60 months were identified by Cox regression analysis (adjusted for gender, age and clinical stage) and retested in a validation series of 78 additional cases. RNA-Seq analysis from non-involved lung tissue of 12 patients was performed to characterize the different isoforms of candidate genes. Ten genes for which the loge-transformed hazard ratios expressed the same direction of effect in the discovery (P<1.0 x 10-3) and validation series comprised the gene expression signature associated with survival: CNTNAP1, PKNOX1, FAM156A, FRMD8, GALNTL1, TXNDC12, SNTB1, PPP3R1, SNX10, and SERPINH1. RNA sequencing highlighted the complex expression pattern of these genes in non-involved lung tissue from different patients, and permitted the detection of a read-through gene fusion between PPP3R1 and the flanking gene (CNRIP1) as well as a novel isoform of CNTNAP1. Our findings support the hypothesis that individual genetic characteristics, evidenced by the expression pattern of non-involved tissue, influence the outcome of lung adenocarcinoma patients.
Galvan A, et al. Carcinogenesis. 2013 Aug 25. [Epub ahead of print]

Solution structure and dynamics of ERp18, a small endoplasmic reticulum resident oxidoreductase

Here we report the solution structure of oxidized ERp18 as determined using NMR spectroscopy. ERp18 is the smallest member of the protein disulfide isomerase (PDI) family of proteins to contain a Cys-Xxx-Xxx-Cys active site motif. It is an 18 kDa endoplasmic reticulum resident protein with unknown function although sequence similarity to individual domains of the thiol-disulfide oxidoreductase PDI suggests ERp18 may have a similar structure and function. Like the catalytic domains of PDI, ERp18 adopts a thioredoxin fold with a thioredoxin-like active site located at the N-terminus of a long kinked helix that spans the length of the protein. Comparison of backbone chemical shifts for oxidized and reduced ERp18 shows the majority of residues possess the same backbone conformation in both states, with differences limited to the active site and regions in close proximity. S(2) order parameters from NMR backbone dynamics were found to be 0.81 for oxidized and 0.91 for reduced ERp18, and these observations, in combination with amide hydrogen exchange rates, imply a more rigid and compact backbone for the reduced structure. These observations support a putative role for ERp18 within the cell as an oxidase, introducing disulfide bonds to substrate proteins, providing structural confirmation of ERp18's role as a thiol-disulfide oxidoreductase.
Rowe ML, et al. Biochemistry. 2009 Jun 2;48(21):4596-606. doi: 10.1021/bi9003342.
  Human ERp18/TLP19 Recombinant
Code No.: 00531-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID:NP_056997
Gene ID: NM_015913
MW: 19 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF


Price ($)
ERp18/TLP19 (Human) rec.
10 ug
ERp18/TLP19 (Human) rec.
50 ug
ERp18/TLP19 (Human) rec.
100 ug
ERp18/TLP19 (Human) rec.. Biotinylated
50 ug
ERp18/TLP19 (Human) rec.. FITC conjugated
50 ug
ERp18/TLP19 (Human) rec.. Cy3 conjugated
50 ug
Anti ERp18/TLP19 (Human) IgG
100 ug
Anti ERp18/TLP19 (Human) IgG Biotinylated
50 ug