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Angiopoietin 1 (ANG-1/ANGPT1)
  • Tie2-ligand
  • ANGPT1
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel
maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic
inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with
consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation,
thus serving as a permissive angiogenic signal

Tissue factor and PAR1 promote microbiota-induced intestinal vascular remodelling

The gut microbiota is a complex ecosystem that has coevolved with host physiology. Colonization of germ-free (GF) mice with a microbiota promotes increased vessel density in the small intestine, , but little is known about the mechanisms involved. Tissue factor (TF) is the membrane receptor that initiates the extrinsic coagulation pathway, and it promotes developmental and tumour angiogenesis. Here we show that the gut microbiota promotes TF glycosylation associated with localization of TF on the cell surface, the activation of coagulation proteases, and phosphorylation of the TF cytoplasmic domain in the small intestine. Anti-TF treatment of colonized GF mice decreased microbiota-induced vascular remodelling and expression of the proangiogenic factor angiopoietin-1 (Ang-1) in the small intestine. Mice with a genetic deletion of the TF cytoplasmic domain or with hypomorphic TF alleles had a decreased intestinal vessel density. Coagulation proteases downstream of TF activate protease-activated receptor (PAR) signalling implicated in angiogenesis. Vessel density and phosphorylation of the cytoplasmic domain of TF were decreased in small intestine from PAR1-deficient but not PAR2-deficient mice, and inhibition of thrombin showed that thrombin–PAR1 signalling was upstream of TF phosphorylation. Thus, the microbiota-induced extravascular TF–PAR1 signalling loop is a novel pathway that may be modulated to influence vascular remodelling in the small intestine.

Reinhardt C., et al. Nature, 483, 627-631, 29 March 2012, doi: 10.1038/nature10893


Human Angiopoietin 1 (ANGPT1) ELISA
Code No.: SK00631-01
Size: 96 T
Price: $360.00 USD
Standard Range:156-10000 pg/ml
Sensitivity:50 pg/ml
Sample Type: cell culture, serum, EDTA plasma
Sample requres: 100uL per well
IntraCV: 4-6%
InterCV: 8-10%
Protocol: PDF

Code No.
Price ($)
Angiopoietin 1 (ANGPT1)  (Human) ELISA
96 T
Angiopoietin 2 (ANGPT1)  (Human) ELISA
96 T
Angiopoietin 3 (ANGPT1)  (Human) ELISA
96 T
Angiopoietin 4 (ANGPT1)  (Human) ELISA
96 T