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Angiotensinogen (AGT)/Serpin A8 ELISA Kit
A biomarker for hypertension

Alternative name:

  • AGT
  • Serpin A8

A polymorphism in AGT and AGTR1 gene is associated with lead-related high blood pressure. Kim HK, et al. J Renin Angiotensin Aldosterone Syst. 2014 Jul 16

Urinary angiotensinogen is a marker for tubular injuries in patients with type 2 diabetes

PURPOSE: Urinary angiotensinogen has been reported as a marker for the activation of intrarenal renin-angiotensin system (RAS) in various kidney diseases. To investigate the importance of urinary angiotensinogen in diabetic nephropathy, we compared the urinary levels of angiotensinogen, albumin, and α1-microglobulin.
MATERIALS AND METHODS:Japanese patients with type 2 diabetes at various stages of nephropathy (n=85) were enrolled, and we measured albumin/creatinine ratio (ACR) and urinary excretion of angiotensinogen and α1-microglobulin. We also compared the clinical data of the patients treated with or without angiotensin II receptor blockers or angiotensin-converting enzyme inhibitors (RAS inhibitors [+], n=51; RAS inhibitors [-], n=34).
RESULTS:Urinary angiotensinogen levels positively correlated with ACR (r=0.367, P=3.84×10(-4)) and urinary α1-microglobulin (r=0.734, P=1.32 × 10(-15)), while they negatively correlated with estimated glomerular filtration ratio (eGFR) (r=-0.350, P=1.02 × 10(-3)) and high-density lipoprotein cholesterol (r=-0.216, P=0.049). Multiple regression analysis was carried out to predict urinary angiotensinogen levels by employing eGFR, ACR, and urinary α1-microglobulin as independent variables; only urinary α1-microglobulin entered the regression equation at a significant level. Although ACR was higher in the RAS inhibitors (+) group, urinary α1-microglobulin and angiotensinogen did not show significant increase in the RAS inhibitors (+) group.
CONCLUSION:Urinary angiotensinogen is well correlated with urinary α1-microglobulin and reflected the tubular injuries which may be associated with the intrarenal RAS activation in patients with type 2 diabetes.
Terami T et al. Int J Nephrol Renovasc Dis. 2013 Oct 22;6:233-40. 

Podocyte injury enhances filtration of liver-derived angiotensinogen and renal angiotensin II generation

Intrarenal angiotensin II is increased in kidney diseases independently of plasma angiotensin II and is thought to promote progressive deterioration of renal architecture. Here we investigated the mechanism of enhanced renal angiotensin II generation in kidney glomerular diseases. For this, kidney- or liver-specific angiotensinogen gene (Agt) knockout was superimposed on the mouse model of inducible podocyte injury (NEP25). Seven days after induction of podocyte injury, renal angiotensin II was increased ninefold in NEP25 mice with intact Agt, accompanied by increases in urinary albumin and angiotensinogen excretion, renal angiotensinogen protein, and its mRNA. Kidney Agt knockout attenuated renal Agt mRNA but not renal angiotensin II, renal, or urinary angiotensinogen protein. In contrast, liver Agt knockout markedly reduced renal angiotensin II to 18.7% of that of control NEP25 mice, renal and urinary angiotensinogen protein, but not renal Agt mRNA. Renal angiotensin II had no relationship with renal Agt mRNA, or with renal renin mRNA, which was elevated in liver Agt knockouts. Kidney and liver dual Agt knockout mice showed phenotypes comparable to those of liver Agt knockout mice. Thus, increased renal angiotensin II generation upon severe podocyte injury is attributed to increased filtered angiotensinogen of liver origin resulting from loss of macromolecular barrier function of the glomerular capillary wall that occurs upon severe podocyte injury.
Matsusaka T et al. Kidney Int. 2013 Nov 27


human angiotensinogen elisa kit from aviscera bioscience Human Angiotensinogen ELISA
Code No.:SK00522-01
Size: 96 T
Price: $420.00 USD
Standard range:234-15000 pg/ml
Sample type: serum, plasma, urine
Dilution Factors:Optimal dilutions should be determined by each laboratory for each application
Specificity: human AGT
Inter-CV: 8-12%
Protocol: PDF
Notice: Research use only



Code No.
Price ($)
Human Angiotensinogen ELISA
96 T
Human Angiotensinogen (HEK293)
5 ug