| Brain-Derived Neurotrophic Factor (BDNF) (Human, Mouse, Rat) ELISA, Recombinant & Antibodies | |
| Pro Brain-Derived Neurotrophic Factor (Pro BDNF) (Human, Mouse, Rat) ELISA, Recombinant & Antibodies | |
| Order BDNF or Pro BDNF ELISA Please Checkout here | |
Crossreactivity of human Pro BDNF (Glycosylated) from human cells was less 1% with Active BDNF ELISA Kit SK00752-01 |
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| Human mature BDNF ELISA Kit SK00572-01 had been used by Dr. Hashimoto on following publications. | |
Serum Levels of Mature Brain-Derived Neurotrophic Factor (BDNF) and Its Precursor proBDNF in Healthy Subjects |
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| Yoshida T., Ishikawa M, Iyo M and Hashimoto K. The Open Clinical Chemistry Joural, 2012, 5, 7-12 | |
| Decreased serum levels of mature brain-derived neurotrophic factor (BDNF), but not its precursor proBDNF, in patients with major depressive disorder | |
| BACKGROUND:Meta-analyses have identified serum levels of brain-derived neurotrophic factor (BDNF) as a potential biomarker for major depressive disorder (MDD). However, at the time, commercially available human ELISA kits are unable to distinguish between proBDNF (precursor of BDNF) and mature BDNF because of limited BDNF antibody specificity. In this study, we examined whether serum levels of proBDNF, mature BDNF, and matrix metalloproteinase-9 (MMP-9), which converts proBDNF to mature BDNF, are altered in patients with MDD.
METHODOLOGY/PRINCIPAL FINDINGS: Sixty-nine patients with MDD and 78 age- and gender-matched healthy subjects were enrolled. Patients were evaluated using 17 items on the Structured Interview Guide for the Hamilton Depression Rating Scale. Cognitive impairment was evaluated using the CogState battery. Serum levels of proBDNF, mature BDNF, and MMP-9 were measured using ELISA kits. Serum levels of mature BDNF in patients with MDD were significantly lower than those of normal controls. In contrast, there was no difference in the serum levels of proBDNF and MMP-9 between patients and normal controls. While neither proBDNF nor mature BDNF serum levels was associated with clinical variables, there were significant correlations between MMP-9 serum levels and the severity of depression, quality of life scores, and social function scores in patients. CONCLUSIONS/SIGNIFICANCE:These findings suggest that mature BDNF may serve as a biomarker for MDD, and that MMP-9 may play a role in the pathophysiology of MDD. Further studies using larger sample sizes will be needed to investigate these results. |
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| Yoshida T et al. PLoS One. 2012;7(8):e42676. Epub 2012 Aug 3. | |
BDNF (Human, Mouse, Rat) ELISA Kit SK00752-01 had been used by Dr. A. Polacchini. The data indicated aviscera's BDNF ELISA Kit SK00752-01 measure the mature form of BDNF. It does not crossreact with human pro BDNF derived from E. Coli on western blot analysis. The recombinant human Pro BDNF derived from HEK293 cells (glycosylated) was less 1% crossreactive with BDNF elisa kit SK00752-01. |
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SK00752-06 was replaced by Pro BDNF (Human) ELISA Kit SK00752-09
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Pro
BDNF (Human) ELISA Kit Code No.: SK00752-09 Size: 96 T Standard range:0.39-25 ng/ml Sensitivity: 0.1 ng/ml Specificity: Pro BDNF (Human) sample require: 100 ul per well Intra-CV: 4-6% Inter-CV: 8-10% Data Sheet: PDF Human Pro BDNF (19-247) derived from E. Coli may not be detected by this elisa kit. |
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Pro
BDNF (Human) ELISA Kit Code No.: SK00752-09 Size: 96 T Standard range:0.39-25 ng/ml Sensitivity: 0.1 ng/ml Specificity: Pro BDNF (Human) sample require: 100 ul per well Intra-CV: 4-6% Inter-CV: 8-10% Data Sheet: PDF Human Pro BDNF (19-247) derived from E. Coli may not be detected by this elisa kit. |
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BDNF (Human, Mouse, Rat) ELISA Kit Code No.: SK00752-01 Size: 96 T Standard range:23-1500 pg/ml Sensitivity: 5-8 pg/ml Specificifty: Mature human BDNF at 100%. Pro BDNF (Human) derived from human cells indicated less 1% crossreactivity. Sample Type: serum Dilution Factor: 20-40 fold dilution for serum samples. Sample require: 6-12 uL of serum Intra-CV: 4-6% Inter-CV: 8-12% Protocol: PDF BDNF (E. Coli derived) may not be detected by this elisa kit |
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BDNF (Human, Mouse, Rat) ELISA Kit Code No.: SK00752-01 Size: 96 T Standard range:23-1500 pg/ml Sensitivity: 5-8 pg/ml Specificifty: Mature form of BDNF at 100%. Pro BDNF (Human) derived from HEK293 cells indicated less 1% crossreactivity. Sample Type: serum Dilution Factor: 20-40 fold dilution for serum samples. Sample require:6-12 uL serum Intra-CV: 4-6% Inter-CV: 8-12% Protocol: PDF Human Pro BDNF (19-247) derived from E. Coli may not be detected by this elisa kit. But the Pro BDNF (Human) derived from HEK293 cells can be detected by this elisa kit. |
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BDNF Ultrasensitive (Human, Mouse, Rat) ELISA Kit Code No.: SK00752-02 Size: 96 T Standard range:11-750 pg/ml Sensitivity: 1-3 pg/ml sample require: 6-12 ul of serum Intra-CV: 4-6% Inter-CV: 8-10% Protocol: PDF |
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Anti Human Pro BDNF Monoclonal Antibody Code No.: A00752-22-100 Size: 100 ug Price: $350.00 USD Host: Mouse Antigen: human Pro BDNF Rec. Specificity: human Pro BDNF (19-247) and Pro BDNF (19-128) only. Ab Type: Monoclonal Clone No.:2B9 Purification: Protein G Applications: E, WB Working Dilution: IHC 1: 500 (2 ug/ml) Data Sheet: PDF |
| Order BDNF or Pro BDNF ELISA Please Checkout here | |
| Name | Code No. |
Size |
| Active BDNF (Human, Mouse, Rat) ELISA Kit | SK00752-01 | 96 T |
| BDNF (Human, Mouse, Rat) Ultrasensitve ELISA Kit | SK00752-02 | 96 T |
| Pro-BDNF (Human) ELISA Kit | SK00752-09 | 96 T |
| Pro-BDNF (Human) ELISA Kit | SK00752-06 | 96 T |
| Goat Anti Pro-BDNF (Human) IgG | A00752-07-100 |
100 ug |
| Goat Anti Pro-BDNF (Human) IgG, Biotinylated | A00752-07-50B |
50 ug |
| Anti Pro BDNF (Human) Monoclonal Antibody | 100 ug |
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| Anti Pro BDNF (Human) Monoclonal Antibody Biotinylated | 50 ug |
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| Pro BDNF (Human) (19-128) Recombinant | 00752-23-10 |
10 ug |
| Pro BDNF (Human) (19-128) Recombinant | 00752-23-50 |
50 ug |
| Pro BDNF (Human, Mouse, Rat) Recombinant (Human cells derived) | 00752-17-05 |
5 ug |
| BDNF (Human, Mouse, Rat) Recombinant (CHO derived) | 00752-21-10 |
10 ug |
| BDNF (Human, Mouse, Rat) Recombinant (CHO derived) | 5 ug |
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| Pro BDNF (19-247) (Human), Rec. | 00752-33-50 |
50 ug |