CXCL1/Growth-regulated alpha protein (GRO-ALPHA) |
CXCL1/GRO-alpha is a newly identified exercise induced myokine |
Exercise-induced liver chemokine CXCL-1 expression is linked to muscle-derived interleukin-6 expression
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The chemokine CXC ligand-1 (CXCL-1) is a small cytokine that elicits effects by signalling through the chemokine receptor CXCR2. CXCL-1 has neutrophil chemoattractant activity, is involved in the processes of angiogenesis, inflammation and wound healing, and may possess neuroprotective effects. The aim of this study was to unravel the mechanisms whereby CXCL-1 is regulated by exercise inmice. After a single bout of exercise, CXCL-1 protein increased in serum(2.4-fold), and CXCL-1 mRNA in muscle (6.5-fold) and liver (41-fold). These increases in CXCL-1 were preceded by increases in serum interleukin-6 (IL-6) and muscle IL-6 mRNA. In contrast, exercise-induced regulation of liver CXCL-1 mRNA expression was completely blunted in IL-6 knockout mice. Based on these findings, we examined the possible existence of a muscle-to-liver axis by overexpressing IL-6 in muscles. This resulted in increases in serum CXCL-1 (5-fold) and liver CXCL-1 mRNA expression (24-fold) compared with control. Because IL-6 expression and release are known to be augmented during exercise in glycogen-depleted animals, CXCL-1 and IL-6 expression were examined after exercise in overnight-fasted mice.We found that fasting significantly augmented serum CXCL-1, and CXCL-1 expression in liver and muscle. Taken together, these data indicate that liver is the main source of serum CXCL-1 during exercise in mice, and that the CXCL-1 expression in the liver is regulated by muscle-derived IL-6. |
Pedersen L et al. J Physiol. 2011 Mar 15;589(Pt 6):1409-20. Epub 2011 Jan 4. |
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Code No.: SK00427-01
Size: 96 T
Price: $360.00 USD
Standard Range:15.6-1000 pg/ml
Sensitivity:10 pg/ml
Sample Type: serum, EDTA plasma
Sample requres: 220 ul, 100 ul per well
IntraCV:6-8%
InterCV: 10-12%
Protocol: PDF |
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Code No.: 00427-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID:P09341
Gene ID: 2919
MW:10 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 90%
Data Sheet: PDF |
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Code No.: A00427-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Antigen: human GRO-alpha.
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: E, IHC, WB
Working Dilution: 2-4 ug/ml)
Data Sheet: PDF |
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Code No.: A00427-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Antigen: human GRO-alpha.
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: E, IHC, WB
Working Dilution: 2-4 ug/ml)
Data Sheet: PDF |