| Endothelial Lipase (EL)/LIPG ELISA, Antibody, Protein | |
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| Endothelial lipase is a major determinant of HDL level | |
| A new member of the gene family, initially termed (gene nomenclature, LIPG; protein, EL), is expressed in a variety of different tissues, suggesting a general role in lipid metabolism. To assess the hypothesis that EL plays a physiological role in lipoprotein metabolism in vivo, we have used gene targeting of the native murine locus and transgenic introduction of the human LIPG locus in mice to modulate the level of EL expression. Evaluation of these alleles in a C57Bl/6 background revealed an inverse relationship between HDL cholesterol level and EL expression. Fasting plasma HDL cholesterol was increased by 57% in LIPG(-/-) mice and 25% in LIPG(+/-) mice and was decreased by 19% in LIPG transgenic mice as compared with syngeneic controls. Detailed analysis of lipoprotein particle composition indicated that this increase was due primarily to an increased number of HDL particles. Phospholipase assays indicated that EL is a primary contributor to phospholipase activity in mouse. These data indicate that expression levels of this novel have a significant effect on lipoprotein metabolism. | |
| Ishida T et al. J Clin Invest.2003 Feb;111(3):347-55. | |
| Expression of Endothelial Correlates with the Size of Neointima in a Murine Model of Vascular Remodeling | |
| im:Endothelial lipase (EL) regulates plasma high-density lipoprotein-cholesterol (HDL-C) levels by promoting HDL catabolism. However, it remains unknown whether the inhibition of EL has beneficial effects on the genesis of vascular diseases. Here, we investigated the role of EL on vascular remodeling in mice.Methods: Vascular remodeling was developed by ligation of the left common carotid artery and neointimal lesions were histologically compared between EL-knockout (ELKO), EL-transgenic (ELTg), and wild-type (WT) mice. HDL was isolated from these mice, and effects of the HDL on cell growth and Erk activation were evaluated in vitro using cultured vascular smooth muscle cells.Results: Plasma HDL-C levels were 62% higher in ELKO and 13% lower in ELTg than in WT mice, after the carotid ligation. The size of neointimal lesion was significantly larger in ELTg and smaller in ELKO than in WT mice. Vascular expression of adhesion molecules was lower in ELKO and higher in ELTg compared with WT mice. Moreover, oxidative stress was attenuated in ELKO mice. HDL isolated from ELKO, ELTg, and WT mice inhibited expression of intercellular adhesion molecule-1, angiotensin II-induced activation of Erk, and growth of cultured vascular smooth muscle cells, whereas EL expression itself did not affect cell migration or growth.Conclusion: EL expression modulates vascular remodeling as well as plasma HDL-C levels. EL inactivation may increase HDL particles that can inhibit smooth muscle cell growth and migration. | |
| Sun L, et al. J Atheroscler Thromb.2012 Sep 10. [Epub ahead of print] | |
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Human Endothelial Lipase (EL) ELISA Kit Code No.: SK00276-08 Size: 96 T Price: $460.00 USD Standard range:15.6-1000 ng/ml Sensitivity: 800 pg/ml Sample Type: serum, plasma, cell cultures Sample require: 200 ul for serum or plasma Intra-CV: 4-8% Inter-CV: 8-10% Data Sheet: PDF |
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Mouse/Rat Endothelial Lipase (EL) ELISA Kit Code No.: SK00276-02 Size: 96 T Price: $460.00 USD Standard range:3.2-2000 ng/ml Sensitivity: 2.5 ng/ml Sample Type: serum, plasma, cell cultures Sample require: 50 ul for serum or plasma Intra-CV: 4-8% Inter-CV: 8-10% Data Sheet: PDF |
| Human Endothelial Lipase Recombinant Code No.: 00276-01-100 Size: 100 ug Price: $360.00 USD Protein ID:Q9Y5X9 Gene ID: 9388 MW: 55.3 KD Tag: His Tag on N-Terminus Expressed: E. Coli Purity: 95% Data Sheet: PDF |
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Human Endothelial Lipase Recombinant Code No.: 00276-02-10 Size: 10 ug Price: $350.00 USD Protein ID:Q9Y5X9 Gene ID:9388 MW:72 KD Tag: His Tag on N-Terminus Expressed: E. Coli Purity: 95% Data Sheet: PDF |
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Anti Human
EL IgG Code No.: A00276-01-100 Size: 100 ug Price: $220.00 USD Host: Rabbit Antigen: human EL Rec. Ab Type: Polyclonal IgG Purification: Protein A Applications: E, IHC Working Dilution: 1-2 ug/ml) Data Sheet: PDF |
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Anti Human
EL IgG Code No.: A00276-01-100 Size: 100 ug Price: $220.00 USD Host: Rabbit Antigen: human EL Rec. Ab Type: Polyclonal IgG Purification: Protein A Applications: E, IHC Working Dilution: 1-2 ug/ml) Data Sheet: PDF |
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Anti Human
EL IgG Code No.: A00276-01-100 Size: 100 ug Price: $220.00 USD Host: Rabbit Antigen: human EL Rec. Ab Type: Polyclonal IgG Purification: Protein A Applications: E, IHC Working Dilution: 1-2 ug/ml) Data Sheet: PDF |
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Anti Human
EL IgG Code No.: A00276-01-100 Size: 100 ug Price: $220.00 USD Host: Rabbit Antigen: human EL Rec. Ab Type: Polyclonal IgG Purification: Protein A Applications: E, IHC Working Dilution: 1-2 ug/ml) Data Sheet: PDF |
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| Name | Code No. |
Size |
Price ($) |
| Endothelial Lipase (Human) ELISA Kit | SK00276-08 | 96 T | 460.00 |
| Endothelial Lipase (Human) ELISA Kit | SK00276-01 | 96 T | 420.00 |
| Endothelial Lipase (Rat/Mouse) ELISA Kit | SK00276-02 | 96 T | 420.00 |
| Endothelial Lipase (Human) ELISA Kit | SK00276-06 | 96 T | 460.00 |
| Endothelial Lipase (Human) , Rec. | 100 ug |
360.00 |
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| Endothelial Lipase (Human) , Rec. | 100 ug |
360.00 |
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| Anti EL (Human) , rabbit IgG , biotinylated | A00276-01-50B |
50 ug |
360.00 |
| AntiEndothelial Lipase (Human) , rabbit IgG | 100 ug |
220.00 |