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Ephrin Type-A Receptor 1 (EphA1) ELISA Kit
A Biomarker for Non-Small Cell Lung Carcinoma

Alternative name:

  • EphA1
  • Ephrin Type-A Receptor 1
  • Oncogene EPH


Eph receptor tyrosine kinases and their ephrin ligands have been implicated in neuronal development and neovascularization. Overexpression ofephrin-A1 has been implicated in tumor progression and poor prognosis. However, the mechanisms are not clear. Here, we report a role of the Eph/ephrin system in a cell adhesion mechanism. Clustered erythropoietin-producing hepatocellular receptor A1 (EphA1)/ephrin-A1 complexes on the plasma membrane did not undergo endocytosis, and the cell remained adherent to one another. The cell-cell contacts were maintained in an Eph tyrosine kinase activity-independent manner even in the absence of E-cadherin. EphA1 and ephrin-A1 co-localized in pulmonary endothelial cells, and regulated vascular permeability and metastasis in the lungs. We identified ADAM12 (A disintegrin and metalloproteinase 12) as an EphA1-binding partner by yeast two-hybrid screening and found that ADAM12 enhanced ephrin-A1 cleavage in response to transforming growth factor-β1 in primary tumors. Released soluble ephrin-A1 in the serum deteriorated the EphA1/ephrin-A1-mediated cell adhesion in the lungs in an endocrine manner, causing lung hyperpermeability that facilitated tumor cell entry into the lungs. Depletion of soluble ephrin-A1 by its neutralizing antibody significantly inhibited lung metastasis.
Ieguchi K1 , et al. Oncogene. 2014 Apr 24;33(17):2179-90. doi: 10.1038/onc.2013.180. Epub 2013 May 20.

Ephrin (Eph) receptor A1, A4, A5 and A7 expression in human non-small cell lung carcinoma: associations with clinicopathological parameters, tumor proliferative capacity and patients' survival

BACKGROUND:Ephrin (Eph) receptors are frequently overexpressed in a wide variety of human malignant tumors, being associated with tumor growth, invasion, metastasis and angiogenesis. The present study aimed to evaluate the clinical significance of EphA1, A4, A5 and A7 protein expression in non-small cell lung carcinoma (NSCLC).
METHODS: EphA1, A4, A5 and A7 protein expression was assessed immunohistochemically in tissue microarrays of 88 surgically resected NSCLC and was analyzed in relation with clinicopathological characteristics and patients' survival.
RESULTS: Elevated EphA4 expression was significantly associated with low histopathological stage and presence of inflammation (p=0.047 and p=0.026, respectively). Elevated EphA7 expression was significantly associated with older patients' age, presence of fibrosis and smaller tumor size (p=0.036, p=0.029 and p=0.018, respectively). EphA1, A5 and A7 expression were positively associated with tumor proliferative capacity (p=0.047, p=0.002 and p=0.046, respectively). Elevated EphA4, A5 and A7 expression were identified as predictors of favourable patients' survival at both univariate (Log-rank test, 0=0.019, p=0.006 and p=0.012, respectively) and multivariate levels (Cox-regression analysis, p=0.029, p=0.068 and p=0.044, respectively).
CONCLUSIONS:The present study supported evidence that Ephs may be involved in lung cancer progression, reinforcing their utility as clinical biomarkers for patients' management and prognosis, as also as potential targets for future therapeutic interventions.
Giaginis C., et al. BMC Clin Pathol. 2014 Feb 4;14(1):8. doi: 10.1186/1472-6890-14-8.
human ephA1 elisa kit from aviscera bioscience enables to measure human samples
Human SolubleEphrin Type-A Receptor 1 (EphA1)ELISA Kit
Code No.: SK00646-01
Size: 96T
Price: $369.00 USD
Standard Range: 39- 2500 pg/mL
Sensitivity:12 pg/mL
Sample Type: serum, EDTA Plasma
Sample volume: 100 uL
Dilution factor: Optimal dilutions should be determined by each laboratory for each application
IntraCV: 4-6%
InterCV: 8-12%
Protocol: PDF


Code No.
Price ($)
Soluble Ephrin Type-A Receptor 1 (EphA1) (Human) ELISA Kit SK00646-01 96 T 369.00
control for soluble EphA1 ELISA Kit
Standard for Soluble EphA1 ELISA Kit
Detection Antibody for Soluble EphA1 ELISA Kit
96 T
Capture Plate for Soluble EphA1 ELISA Kit
96 T