FDC-SP ELISA |Recombinant

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Follicular Dendritic Cell Secreted Protein (FDC-SP) Recombinant, Antibody & ELISA Kit
A small secreted protein of expressed in human tonsillar germinal centers (GC) has immunomodulatory activities on B cells.
Follicular dendritic cell-secreted protein may enhance osteoclastogenesis in periodontal disease. Liu J, et al. Connect Tissue Res. 2016 Feb;57(1):38-43
Decreased expression of follicular dendritic cell-secreted protein correlates with increased immunoglobulin A production in the tonsils of individuals with immunoglobulin A nephropathy. Meng HX, et al. Transl Res. 2015 Sep;166(3):281-91
Transfection with follicular dendritic cell secreted protein to affect phenotype expression of human periodontal ligament cells.
Follicular dendritic cell secreted protein (FDC-SP), has been found to inhibit osteogenic differentiation of human periodontal ligament cells (hPDLCs) in recent studies. Based on these findings, we further investigate its effect on phenotype expression of hPDLCs in the present study, aiming to contribute to a better understanding of the biological functions governing FDC-SP-induced hPDLC differentiation. hPDLCs were firstly identified with immunocytochemical staining, followed by transfection with FDC-SP lentiviral vector. Western blot analysis was used to confirm the expression of FDC-SP. Then the influence of FDC-SP transfection on hPDLC proliferation, osteogenic and fibrogenic phenotype expression was evaluated at the mRNA and protein level. Procollagen type I c-peptide production was measured and alizarin red staining was then conducted to demonstrate effect of FDC-SP on functional differentiation. We found that hPDLCs could be successfully transfected with FDC-SP. Cell proliferation and cell cycle tests indicated that transfection with FDC-SP did not affect hPDLC proliferation. Moreover, according to real-time PCR and Western blot results, expression levels of type 1 collagen alpha 1, type 1 collagen alpha 2 and type 3 collagen were upregulated while that of osteocalcin, osteopontin, and bone sialoprotein were downregulated in FDC-SP transfected cells. In addition, hPDLCs overexpressing FDC-SP exhibited higher PIP production than the controls. Our findings demonstrate that transfection with FDC-SP has negligible adverse effect on proliferation of hPDLCs and imply the biological function of FDC-SP as a fibroblastic phenotype stabilizer by inhibiting hPDLCs differentiation into mineralized tissue-forming cells, thus regulating regeneration in periodontal tissue engineering
Xiang L, et al. J Cell Biochem. 2014 May;115(5):940-8
Effect of FDC-SP on the phenotype expression of cultured periodontal ligament cells
INTRODUCTION: Recently, a novel protein, follicular dendritic cell secreted protein (FDC-SP), has been identified in human periodontal ligament (PDL) tissue and a biomolecular study suggested that the expression of FDC-SP might be associated with the expression of the PDL phenotype. The purpose of this study was to test the effect of FDC-SP on the proliferation and phenotype of PDL cells. MATERIAL AND METHODS: Periodontal ligament cells obtained following the 3(rd) passage were exposed to various concentrations of FDC-SP. The cell proliferation was monitored by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide(MTT) assay. Then, as a measure of osteogenic activity, the alkaline phosphatase (ALP) activity was recorded after 4, 7, and 14 days using p-nitrophenylphosphate as a substrate. Finally, total RNA was extracted and RT-PCR was performed for gene analysis. RESULTS: The results indicated that PDL cells exposed to 50 ng/ml FDC-SP could proliferate more rapidly. RT-PCR results showed that the mRNA expression of epidermal growth factor receptor (EGFR) was obviously upregulated and the mRNA expression of osteocalcin (OCN) and bone sialoprotein (BSP) were downregulated in PDL cells exposed to FDC-SP. Moreover, two groups of PDL cells exposed to FDC-SP showed a significant decrease of ALP activity during all the culture days. CONCLUSIONS: In sum, the findings observed in this study suggest that FDC-SP in PDL cells could positively affect the proliferation and act as a fibroblastic phenotype stabilizer by inhibiting their differentiation into mineralized tissue-forming cells.
Wei N, et al. Arch Med Sci. 2011 Apr;7(2):235-41. doi: 10.5114/aoms.2011.22073. Epub 2011 May 17.

	FDC SP (Human)ELISA Kit Code No.: SK00319-01 Size: 480 T Price: $2200.00 USD Standard Range: 50-800 ng/ml Sensitivity: 10 ng/ml Sample Type: recombinant protein derived from E. Coli Sample require: 100ul per well Data Sheet: PDF
	Anti FDC SP (Human)IgG Code No.: A00319-01-100 Size: 100 ug Price: $460.00 USD Host: Rabbit Antigen:FDC SP (H) Rec. Ab Type: Polyclonal IgG Purification: Protein A Applications: indirectly E, IHC, Working Dilution: IHC 1: 500 (2 ug/ml) Data Sheet: PDF
	Human FDC SP Recombinant Code No.: 00319-01-100 Size: 100 ug Price: $460.00 USD Protein ID:Q8NFU4 Gene ID:260436 MW:7.5 KD Tag: His Tag on N-Terminus Expressed: E. Coli Purity: 95% Data Sheet: PDF

Name	Code No.	Size	Price ($)
FDC-SP (Human) ELISA Kit	SK00319-01	480 T	2220.00
FDC SP (Human) Rec.	00319-01-100	100 ug	460.00
FDC SP (Human) Rec.	00319-01-1000	1000 ug	4200.00
FDC SP (Human) Rec.. Biotinylated	00319-01-50B	50 ug	560.00
Anti Human FDC SP Rabbit IgG	A00319-01-100	100 ug	460.00
Anti Human FDC SP Rabbit IgG Biotinylated	A00319-01-50B	50 ug	660.00