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FGF5 Protein

Role of Fibroblast Growth Factor-5 on the Proliferation of Human Tonsil-derived Mesenchymal Stem Cells

Human mesenchymal stem cells (MSCs) are a promising tool for therapeutic applications in cell-based therapy and regenerative medicine, and MSCs from the human palatine tonsils have recently been used as a new tissue source. However, the understanding of the proliferation and differentiation capacity of T-MSCs is limited. In this study, we compared the proliferative potential of T-MSCs with BM-MSCs and adipose tissue-derived MSCs (A-MSCs). Additionally, we investigated the underlying mechanism of T-MSC function. We showed that T-MSCs proliferated faster than A-MSCs and BM-MSCs in MTT assays, cell count assays, and cell cycle distribution analyses. DNA microarray and real-time PCR analyses revealed that the expression of fibroblast growth factor-5 (FGF5) was significantly elevated in T-MSCs compared with those in A-MSCs and BM-MSCs. Cell growth curves showed a difference in cell growth between untreated cells and siFGF5-treated T-MSCs. The administration of recombinant human FGF5 (rhFGF5) to the cells transfected with siFGF5 led to a significant increase in the proliferation rates. The administration of rhFGF5 to T-MSCs led to an increase in the levels of phosphorylated ERK1/2. However, treatment with siFGF5 resulted in an overall decrease in the level of phosphorylated ERK1/2. The osteogenic differentiation of T-MSCs was reduced following siFGF5 transfection, and it recovered to near-normal levels when rhFGF5 was added. These findings indicate that T-MSCs show significantly higher proliferative potential compared with BM-MSCs and A-MSCs. FGF5 facilitates cell proliferation through ERK1/2 activation, and it influences the osteogenic differentiation of T-MSCs.
Park GC, et al Stem Cells Dev. 2016 May 25. [Epub ahead of print]

human FGF5 recombinant

Human FGF5 Recombinant
Code No.: 00351-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID: P12034
Gene ID: 2250
MW: 28 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 90%
Data Sheet: PDF

Anti Human FGF5 IgG
Code No.: A00351-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Antigen: human FGF1 Rec.
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: E, IHC, WB
Working Dilution: IHC 1: 200 (5 ug/ml)
Data Sheet: PDF



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