| FGF5 Protein | |
Role of Fibroblast Growth Factor-5 on the Proliferation of Human Tonsil-derived Mesenchymal Stem Cells |
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| Human mesenchymal stem cells (MSCs) are a promising tool for therapeutic applications in cell-based therapy and regenerative medicine, and MSCs from the human palatine tonsils have recently been used as a new tissue source. However, the understanding of the proliferation and differentiation capacity of T-MSCs is limited. In this study, we compared the proliferative potential of T-MSCs with BM-MSCs and adipose tissue-derived MSCs (A-MSCs). Additionally, we investigated the underlying mechanism of T-MSC function. We showed that T-MSCs proliferated faster than A-MSCs and BM-MSCs in MTT assays, cell count assays, and cell cycle distribution analyses. DNA microarray and real-time PCR analyses revealed that the expression of fibroblast growth factor-5 (FGF5) was significantly elevated in T-MSCs compared with those in A-MSCs and BM-MSCs. Cell growth curves showed a difference in cell growth between untreated cells and siFGF5-treated T-MSCs. The administration of recombinant human FGF5 (rhFGF5) to the cells transfected with siFGF5 led to a significant increase in the proliferation rates. The administration of rhFGF5 to T-MSCs led to an increase in the levels of phosphorylated ERK1/2. However, treatment with siFGF5 resulted in an overall decrease in the level of phosphorylated ERK1/2. The osteogenic differentiation of T-MSCs was reduced following siFGF5 transfection, and it recovered to near-normal levels when rhFGF5 was added. These findings indicate that T-MSCs show significantly higher proliferative potential compared with BM-MSCs and A-MSCs. FGF5 facilitates cell proliferation through ERK1/2 activation, and it influences the osteogenic differentiation of T-MSCs. | |
| Park GC, et al Stem Cells Dev. 2016 May 25. [Epub ahead of print] | |
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Human FGF5 Recombinant Code No.: 00351-01-100 Size: 100 ug Price: $360.00 USD Protein ID: P12034 Gene ID: 2250 MW: 28 KD Tag: His Tag on N-Terminus Expressed: E. Coli Purity: 90% Data Sheet: PDF |
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Anti Human FGF5 IgG Code No.: A00351-01-100 Size: 100 ug Price: $220.00 USD Host: Rabbit Antigen: human FGF1 Rec. Ab Type: Polyclonal IgG Purification: Protein A Applications: E, IHC, WB Working Dilution: IHC 1: 200 (5 ug/ml) Data Sheet: PDF |
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| More FGF family Proteins |
| Item | Code |
Size |
Price (USD) |
| Human FGF-5 Rec | 00351-01-10 |
10 ug |
120.00 |
| Human FGF-5 Rec | 100 ug |
360.00 |
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| Human FGF-5 Rec | 10 x 100 ug |
inquire |
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| Anti Human FGF-5 IgG | 100 ug |
260.00 |
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