| Glia Maturation Factor Gamma (GMF-G) | |
| Biomarker for Tumor | |
Alternative name:
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| Glia maturation factor-gamma is preferentially expressed in microvascular endothelial and inflammatory cells and modulates actin cytoskeleton reorganization | |
| Actin cytoskeleton reorganization is a fundamental process for actin-based cellular functions such as cytokinesis, phagocytosis, and chemotaxis. Regulating actin cytoskeleton reorganization is therefore an attractive approach to control endothelial and inflammatory cells function and to treat cardiovascular diseases. Here, we identified glia maturation factor-gamma (GMFG) as a novel factor in actin cytoskeleton reorganization and is expressed preferentially in microvascular endothelial and inflammatory cells. During mouse embryogenesis, GMFG was expressed predominantly in blood islands of the yolk sac, where endothelial and hematopoietic cells develop simultaneously. In endothelial cells, GMFG was colocalized with F-actin in membrane ruffles and was associated with F-actin assessed by actin co-sedimentation assay. Interestingly, GMFG was phosphorylated at N-terminal serine, and its phosphorylation was enhanced by coexpression of dominant active Rac1 and Cdc42. Furthermore, a pseudophosphorylated form of GMFG (GMFG-S2E) demonstrated higher association with F-actin. Stable expression of GMFG-S2E remarkably enhanced stimulus-responsive lamellipodia and subsequent membrane ruffle formation in HeLa cells presumably through its interaction with Arp2/3 complex. Expression of GMFG enhanced actin-based cellular functions such as migration and tube-formation in endothelial cells. Moreover, we found that GMFG expression was significantly increased in a cardiac ischemia/reperfusion model where inflammation and angiogenesis take place actively. Taken together, our findings define a novel pathway in the regulation of actin-based cellular functions. Regulating GMFG function may provide a novel approach to modulate the pathophysiology of cardiovascular diseases. | |
| Ikeda K, et al. Circ Res. 2006 Aug 18;99(4):424-33. Epub 2006 Jul 27. | |
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Anti Human
GMF-G Monoclonal Antibody Code No.: A00676-02-100 Size: 100 ug Price: $260.00 USD Host: Mouse Antigen: human GMF-G Rec. Ab Type: Monoclonal Clone No.: 3H8H11C2 Purification: Protein A Applications: E, WB Working Dilution: 2 ug/ml) Data Sheet: PDF |
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Anti Human
GMF-G Monoclonal Antibody Code No.: A00676-03-100 Size: 100 ug Price: $260.00 USD Host: Mouse Antigen: human GMF-G Rec. Ab Type: Monoclonal Clone: 1B6C6D2F3 Purification: Protein A Applications: E, WB Working Dilution: 2 ug/ml) Data Sheet: PDF |
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Anti Human
GMF-G IgG Code No.: A00676-01-100 Size: 100 ug Price: $220.00 USD Host: Rabbit Antigen: human GMF-G Rec. Ab Type: Polyclonal IgG Purification: Protein A Applications: E, IHC Working Dilution: 2 ug/ml) Data Sheet: PDF |
| Human
GMF-G Recombinant Code No.: 00676-01-100 Size: 100 ug Price: $360.00 USD Protein ID:O60234 Gene ID: 9535 MW:15.5 KD Tag: His Tag on N-Terminus Expressed: E. Coli Purity: 80% Data Sheet: PDF |
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| Name | Code No. |
Size |
Price ($) |
| GMF-G (Human) recombinant | 00676-01-10 |
10 ug |
80.00 |
| GMF-G (Human) recombinant | 00676-01-50 |
50 ug |
250.00 |
| GMF-G (Human) recombinant | 100 ug |
360.00 |
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| GMF-G (Human) recombinant | 00676-01-1000 |
1000 ug |
3200.00 |
| Rabbit Anti GMF-G (Human) IgG | 100 ug |
220.00 |
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| Rabbit Anti GMF-G (Human) IgG | 5 mL |
200.00 |
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| Anti MGF-G (Human) Monoclonal Antibody | A00676-02-100 | 100 ug | 260.00 |
| Anti MGF-G (Human) Monoclonal Antibody | A00676-03-100 | 100 ug | 260.00 |
| GMF-G Western Blot Kit | WK00676-02 | 5-runs | 560.00 |
| GMF-G Western Blot Kit | WK00676-03 | 5 runs | 560.00 |
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