| Interleukin 8 (IL-8) ELISA | |
| A Ligand for CXCR1 and CXCR2 | |
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| IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. | |
Elevated IL-8 and Th1 and Th17 cytokine levels prior to antiretroviral therapy in participants who developed IRIS during ACTG A5164 |
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| Background. Immune reconstitution inflammatory syndrome (IRIS) reflects an aberrant immune response that can develop in HIV-infected patients initiating antiretroviral therapy (ART). Its pathogenesis remains unclear.Methods. We performed a nested case-control study using specimens from ACTG A5164. We compared plasma biomarkers and T-cell subsets in 19 IRIS and 39 control participants at study entry, ART initiation, and IRIS and used conditional logistic regression to develop IRIS predictive models. We evaluated corticosteroids' effects on biomarker levels.Results. Eleven and eight participants developed paradoxical and unmasking IRIS, respectively, none while still receiving corticosteroids. Compared to controls, cases displayed elevations at study entry in IL-8, Th1 (IL-2, IFN-γ, TNF) and Th17 (IL-17) cytokine levels that persisted through ART initiation and IRIS. In logistic regression, baseline higher IFN-γ and TNF were strong predictors of IRIS. Participants who received corticosteroids and later developed IRIS had marked increases in IL-6, IL-8, and IFN-γ at the time of IRIS. T-cell activation markers did not differ in cases and controls prior to ART but were increased in cases at the time of IRIS.Conclusions. Increased IL-8, Th1 and Th17 cytokine levels in IRIS patients precede ART initiation and could help identify patient populations at higher risk for IRIS. | |
| Grant PM ET AL. J Infect Dis. 2012 Sep 21. [Epub ahead of print] | |
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Human IL-8 ELISA Kit Code No.: SK00289-02 Size: 96 T Price: $360.00 USD Standard range:7.8-250 pg/ml Sensitivity: 2-3 pg/ml Sample Type: serum, plasma, cell cultures Sample require:100 ul per well Intra-CV: 4-8% Inter-CV: 8-10% Data Sheet: PDF |
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| Name | Code No. |
Size |
Price ($) |
| Interleukin 8 (IL-8) (Human) ELISA Kit | SK00289-02 | 96 T | 360.00 |
| IL-8 (Human) Rec | 00289-01-100 | 100 ug | 360.00 |
| Anti IL-8 (Human) ELISA kit | A00289-01-100 | 100 ug | 220.00 |