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Human Soluble RANK ELISA Kit SK00657-01 was used by Dr. Kuroki H on following paper.

Identification of marker proteins by orthodontic treatment: relationship of RANKL in the gingival crevicular fluidand of amylase in whole saliva with orthodontic treatment

Orthodontic medical treatment is performed to move a tooth to the optimal position to obtain optimal occlusion. Orthodontic treatment is accompanied by mechanical stress due to orthodontic force and by psychological stress that is experienced as pain or displeasure. The purpose of this study was to identify stress marker proteins during orthodontic treatment. Levels of receptor activator of NFκB (RANKL) and heat shock protein 70 (HSP70) in the gingival crevicular fluid (GCF) were analyzed as markers of mechanical stress, and levels of chromogranin A (CgA) and amylase in whole salivawere analyzed as markers of psychological stress. GCF was collected from control and experimental teeth at initiation of treatment and 24 h aftertreatment. Whole saliva was collected before treatment, at initiation of treatment and 24 h after treatment. RANKL was expressed at 24 h aftertreatment in the experimental GCF, but not in the control GCF. HSP70 appeared to be constitutively expressed in GCF, and its levels showed no major change between the control and experimental groups from initiation of treatment to 24 h after treatment. Amylase activity in whole saliva was enhanced at 24 h after treatment compared to control, but CgA levels showed little change between the groups. These results indicated that RANKLand amylase may be the candidate markers for mechanical and psychological stress, respectively, during orthodontic treatment, even though the total protein concentration and amylase activity displayed a large standard deviation among subjects. Further studies are therefore required to establish these markers for clinical use.
Kuroki H, et al. Odontology. 2013 Jul 18. [Epub ahead of print]
human rank elisa kit from aviscera bioscience Human Soluble RANK ELISA
Code No.: SK00657-01
Size: 96 T
Price: $360.00 USD
Standard Range:62-4000 pg/ml
Sensitivity:30 pg/ml
Sample Type: serum, plasma
Dilution factor: N/A
Sample requres: 100uL per well ,
InterCV: 10-12%
Protocol: PDF


Price ($)
Human Soluble RANK ELISA Kit
96 T