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Wnt Inhibitory Factor 1 LISA

Alternative name:

  • WIF-1
Wnt inhibitory factor 1 is a lipid-binding protein and secreted extracellular signaling molecules to involve in the control of embryonic development. WIF-1 binds Wnt proteins and inhibits their activities.

Prognostic value of Wnt inhibitory factor-1 expression in hepatocellular carcinoma that is independent of gene methylation

Recently, Wnt inhibitory factor-1 (WIF-1) was found to be epigenetically inactivated in several solid tumors, but the biological and clinical relevance ofWIF-1 methylation and expression status in hepatocellular carcinoma (HCC) are still unclear. In the present study, reverse transcription polymerase chain reaction (PCR) and methylation-specific PCR were used to examine the WIF-1 expression and methylation in HCC cell lines. In addition, methylation and expression status of WIF-1 in 105 HCC cases were correlated with clinicopathological parameters and prognosis after tumor resection. WIF-1 was expressed in one HCC cell line and L02, both of which were not methylated in promoter region. DNA hypermethylation of WIF-1promoter was identified in the other four HCC cell lines without WIF-1 expression. In neoplastic and non-neoplastic tissue samples, the rates of WIF-1methylation were 61.9% and 37.1% (P = 0.001), respectively. WIF-1 was significantly downregulated in neoplastic tissues at messenger ribonucleic acid (mRNA) level, as compared to adjacent non-neoplastic tissues (P = 0.006). A significant inverse association was observed between WIF-1methylation of and WIF-1 expression (P  0.017, R = -0.232). Methylation of WIF-1 was not associated with patient survival. In contrast, patients whose tumors exhibited negative WIF-1 mRNA expression had lower rates of overall survival. These findings suggested that aberrant methylation of WIF-1 is a common event in hepatocarcinogenesis. In addition, expression, but not methylation, of WIF-1 is a predictor of good outcome in patients undergoing resection of HCC.
Huang L, et al.Tumour Biol. 2011 Feb;32(1):233-40. Epub 2010 Oct 30.
  Human WIF-1 ELISA
Code No.: SK00508-01
Size: 96 T
Price: $360.00 USD
Standard Range:62.5-2000 pg/ml
Dynamic Range: 62.5-2000 pg/ml
Sensitivity: 10-31 g/ml
Sample Type: serum, EDTA plasma
Dilution factor:
IntraCV: 4-6%
InterCV: 8-10%
Protocol: PDF


Code No.
Price ($)
WIF-1 (Human) ELISA Kit
96 T