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Ciliary Neurotrophic Factor (CNTF) ELISA Kit, Protein, Antibody |
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. |
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
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Inactivation of cardiotrophin-like cytokine, a second ligand for ciliary neurotrophic factor receptor, leads to cold-induced sweating syndrome in a patient |
Ciliary neurotrophic factor (CNTF) receptor controls a pathway supporting the differentiation and survival of a wide range of neural cell types during development and in adulthood. Cardiotrophin-like cytokine (CLC)-cytokine-like factor 1 (CLF) composite cytokine is a second ligand for the CNTF alpha-component receptor (CNTFRalpha). This composite cytokine is built on the structural model of IL-12, with a complex formed by a four-helix bundle type I cytokine, CLC (also referred to as CLCF1), bound to a soluble receptor subunit, CLF (also known as CRLF1). We have reported mutations in the chaperone soluble receptor CLF, causing cold-induced sweating syndrome (CISS). In this study, we studied the CLC-mutated alleles in a patient suffering from a similar disease. This patient was compound heterozygous for two different CLC mutations. The first allele was inactivated by a stop codon at position 107 (Y107X). In the second allele, a R197L mutation in the CLC-predicted binding site to the CNTFRalpha was detected. Functional analysis of the mutated protein revealed an incapacity for R197L CLC to bind to CNTFRalpha and activate the subsequent signaling events. Structural and docking interaction studies showed that the R197L substitution destabilized the contact site between CLC and CNTFRalpha. |
Rousseau F, , et al.Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):10068-73. Epub 2006 Jun 16. |
The novel mechanism of recombinant human ciliary neurotrophic factor on the anti-diabetes activity |
In a previous study, the ciliary neurotrophic factor (CNTF) were demonstrated to lead to weight-loss partly by up-regulating the energy metabolism and the expression of uncoupling protein-1, mitochondrial transcription factor A and nuclear respiratory factor-1 in adipose tissues or muscle. To investigate the up-stream regulators of the expression, recombinant human CNTF (rhCNTF) (0.1, 0.3 and 0.9 mg/kg/day subcutaneously) were administered to KK-Ay mice for 30 days, resulting in reduction of perirenal fat mass, serum free fatty acids and islet triacylglycerol; furthermore, the values of oral glucose tolerance test were found improved. In brown adipose tissues, the gene expressions of peroxisome proliferator-activated receptor alpha (PPARalpha) and peroxisome proliferator-activated receptor coactivator-1 alpha (PGC-1alpha) were found to be up-regulated by rhCNTF. To the best of our knowledge, the changes of gene expression of PPARalpha and PGC-1alpha represent new insights into the mechanisms of anti-diabetes by rhCNTF. In addition, the activity of mitochondrial complexII was found to be increased by rhCNTF. Stimulation of PPARalpha, PGC-1alpha, uncoupling protein-1 and enhanced activity of mitochondrial complex II may be associated with the effects of anti-diabetes. The present study indicates new mechanisms of the activity and mechanisms on anti-diabetes of rhCNTF, which may be a novel anti-diabetes reagent partly acting by enhancing energy metabolism. |
Liu QS, et al. Basic Clin Pharmacol Toxicol. 2007 Aug;101(2):78-84. |
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Code No.: SK00751-01
Size: 96 T
Price: $360.00 USD
Standard Range:46-3000 pg/ml
Sensitivity:20 pg/ml
Sample Type: cell cultures
Sample requres: 100 ul per well
IntraCV: 6-8%
InterCV: 10-12%
Protocol: PDF |
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Code No.: A00751-01-100
Size: 100 ug
Price: $220.00 USD
Host: Rabbit
Antigen: human CNTF Rec.
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: E, IHC, WB
Working Dilution: IHC 5 ug/ml)
Data Sheet: PDF |
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Code No.: 00751-01-100
Size: 100 ug
Price: $360.00 USD
Protein ID:P26441
Gene ID: 1270
MW:25 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 90%
Data Sheet: PDF |
More IL-6 superfamily and Adiponectin related Proteins |
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Name |
Code No. |
Size |
Price ($) |
Human CNTF ELISA Kit |
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96 T |
360.00 |
Ciliary Neurotrophic Factor (CNTF) (Human) , Rec. |
00751-01-10 |
10 ug |
130.00 |
Ciliary Neurotrophic Factor (CNTF) (Human) , Rec. |
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50 ug |
220.00 |
Ciliary Neurotrophic Factor (CNTF) (Human) , Rec. |
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100 ug |
360.00 |
Ciliary Neurotrophic Factor (CNTF) (Human) , Rec. |
00751-01-1000 |
1000 ug |
inquire |
Ciliary Neurotrophic Factor (CNTF) (Human) , Rec. Biotinylated |
00751-01-50B |
50 ug |
460.00 |
Anti Ciliary Neurotrophic Factor (CNTF) (Human) , IgG |
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100 ug |
220.00 |
Ciliary Neurotrophic Factor (CNTF) (Human) , Rec. (Human cells derived) |
00751-02-10 |
10 ug |
190.00 |
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