| Elastin (Soluble) ELISA Kit | |
| Soluble Elastin is a Biomarker for Stabilization of Atherosclerotic Plaque of Atherosclerosis | |
Soluble elastin decreases in the progress of atheroma formation in human aorta. Akima T et al. Circ J. 2009 Nov;73(11):2154-62 |
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The anti-inflammatory effects of exercise training promote atherosclerotic plaque stabilization in apolipoprotein E knockout mice with diabetic atherosclerosis |
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| Physical exercise is the cornerstone of cardiovascular disease treatment. The present study investigated whether exercise training affectsatherosclerotic plaque composition through the modification of inflammatory-related pathways in apolipoprotein E knockout (apoE(-/-)) mice with diabetic atherosclerosis. Forty-five male apoE(-/-) mice were randomized into three equivalent (n=15) groups: control (CO), sedentary (SED), and exercise (EX). Diabetes was induced by streptozotocin administration. High-fat diet was administered to all groups for 12 weeks. Afterwards, CO mice were euthanatized, while the sedentary and exercise groups continued high-fat diet for 6 additional weeks. Exercising mice followed an exercise program on motorized-treadmill (5 times/week, 60 min/session). Then, blood samples and atherosclerotic plaques in the aortic root were examined. A considerable (P<0.001) regression of the atherosclerotic lesions was observed in the exercise group (180.339 ± 75.613 x10(3)μm(2)) compared to the control (325.485 ± 72.302 x10(3)μm(2)) and sedentary (340.188 ± 159.108 x 10(3)μm(2)) groups. We found decreased macrophages, matrix metalloproteinase-2 (MMP-2), MMP-3, MMP-8 and interleukin-6 (IL-6) concentrations (P<0.05) in the atherosclerotic plaques of the exercise group. Compared to both control and sedentary groups, exercise training significantly increased collagen (P<0.05), elastin (P<0.001), and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) (P<0.001) content in the atherosclerotic plaques. Those effects paralleled with increased fibrous cap thickness and less internal elastic lamina ruptures after exercise training (P<0.05), while body-weight and lipid parameters did not significantly change. Plasma MMP-2 and MMP-3 concentrations in atherosclerotic tissues followed a similar trend. From our study we can conclude that exercise training reduces and stabilizes atherosclerotic lesions in apoE-/- mice with diabetic atherosclerosis. A favorable modification of the inflammatory regulators seems to explain those beneficial effects. | |
| Kadoglou NP et al. Eur J Histochem. 2013 Mar 25;57(1) | |
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Human Elastin (Soluble)
ELISA Kit Code No.: SK00806-01 Size: 96 T Price: $460.00 USD Standard Range:0.64-2000 ng/ml Sample Type: serum Sample require: 50 uL per well Intra-CV: 4-6% Inter-CV: 8-10% Protocol: PDF |
| Name | Code No. |
Size |
Price ($) |
| Elastin (Soluble) (Human) ELISA Kit | 96 T |
460.00 |
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