CTRP5 (Human) ELISA Kit, Antibody, Proteins |
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Regulation and function of C1Q/TNF-related protein-5 (CTRP-5) in the context of adipocyte biology
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BACKGROUND AND AIM:
The C1q/TNF-related protein (CTRP) family represents a growing family of adiponectin paralogous proteins. CTRP-5 was detected in adipose tissue of mice and plays a role in the context of the metabolic syndrome. It was the aim to investigate the detailed expression profile of CTRP-5 in a variety of adipocytic cells and to determine whether CTRP-5 circulates in human serum. Moreover, regulation and function of CTRP-5 was studied in the context of adipocyte biology.
MATERIAL AND METHODS:
CTRP-5 serum levels were measured in 50 healthy subjects by ELISA. Genotype analysis was performed by direct sequencing in 200 probands. CTRP-5 mRNA and protein expression was analyzed by RT-PCR, real-time RT-PCR and Western blot. Recombinant CTRP-5 and fatty acids were used for stimulation experiments in 3T3-L1 adipocytes. siRNA-mediated knockdown of CTRP-3 was performed during adipocyte differentiation.
RESULTS:
CTRP-5 mRNA and protein was strongly expressed in a wide variety of human and murine adipocytic cells and was induced during adipocyte differentiation. Saturated fatty acids increased CTRP-5 expression in adipocytes. siRNA-mediated cellular knockdown of CTRP-3 in adipocytes resulted in an upregulation of CTRP-5 expression. CTRP-5 inhibited the release of resistin and adiponectin dose-dependently. CTRP-5 circulates abundantly in human sera with a broad interindividual variation. The SNP 1014 T/A was not associated with type 2 diabetes mellitus in 200 Caucasian probands.
CONCLUSIONS:CTRP-5 might be a novel adipokine that circulates abundantly in human sera. CTRP-5 is functionally involved in adipocyte biology and there might exist a counter-regulatory connection with its family member, CTRP-3
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Schmid A et al. Exp Clin Endocrinol Diabetes. 2013 May;121(5):310-7 |
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Code No.: SK00594-06
Size: 96 T
Price: $420.00 USD
Standard range: 1.56-200 ng/ml
Sensitivity:500 pg/ml
Sample Type: serum, EDTA plasma
Sample require: 100 uL per well
Intra-CV:6-8%
Inter-CV: 8-12%
Protocol: PDF |
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Code No.: SK00594-08
Size: 96 T
Price: $420.00 USD
Standard range: 1.56-200 ng/ml
Sensitivity:200 pg/ml
Sample Type: serum, plasma
Sample require: 100 uL per well
Intra-CV:6-8%
Inter-CV: 8-12%
Protocol: PDF |
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Code No.: A00594-02-100
Size: 100 ug
Price: $280.00 USD
Host: Rabbit
Antigen: human CTRP5 Rec.
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: E, IHC, WB
Working Dilution: 2-4 ug/ml)
Data Sheet: PDF |
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Code No.: 00594-01-100
Size: 100 ug
Price: $390.00 USD
Protein ID:NP_056460
Gene ID:NM_015645
MW:27 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF |
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Code No.: 00594-02-100
Size: 100 ug
Price: $360.00 USD
Protein ID:Q9BXJ2
Gene ID:114905
MW:16 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF |
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Name |
Code No. |
Size |
Price ($) |
CTRP5 (Human) ELISA Kit |
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96 T |
420.00 |
CTRP5 (Human) ELISA Kit |
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96 T |
420.00 |
Anti CTRP5 (Human) Antibody |
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100 ug |
280.00 |
Anti CTRP5 (Human) Antibody Biotinylated |
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50 ug |
330.00 |
Anti CTRP5 (Human) Antibody |
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100 ug |
280.00 |
CTRP5 Full Length (Human) rec |
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100 ug |
390.00 |
CTRP5 Full Length (Human) rec |
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50 ug |
280.00 |
CTRP5 Globular Form (Human) rec |
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100 ug |
360.00 |
CTRP5 Globular Form (Human) rec |
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100 ug |
260.00 |
Anti CTRP5 (Human) Mab |
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100 ug |
280.00 |
Anti CTRP5 (Human) Mab Biotinylated |
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50 ug |
350.00 |
Anti CTRP5 (Human) Mab |
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100 ug |
280.00 |
Anti CTRP5 (Human) Mab Biotinylated |
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50 ug |
350.00 |
Anti CTRP5 (Human) Mab |
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100 ug |
280.00 |
Anti CTRP5 (Human) Mab Biotinylated |
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50 ug |
350.00 |
Anti CTRP5 (Human) Mab |
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100 ug |
280.00 |
Anti CTRP5 (Human) Mab Biotinylated |
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50 ug |
350.00 |
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